ADPGK (NM_031284) Human Tagged ORF Clone

CAT#: RC202965

ADPGK (Myc-DDK-tagged)-Human ADP-dependent glucokinase (ADPGK), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY410576) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202965 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ADPGK protein (Cat# TP302965). The protein was produced from HEK293T cells transfected with ADPGK cDNA clone (Cat# RC202965) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ADPGK
• Synonyms: 2610017G09Rik; ADP-GK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202965 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCTGTGGCGCGGCTCCGCGTACGCGGGCTTCCTGGCGCTGGCCGTGGGCTGCGTCTTCCTGCTGG
  AGCCAGAGCTGCCAGGCTCGGCGCTGCGCTCTCTCTGGAGCTCGCTGTGTCTGGGGCCCGCGCCTGCGCC
  CCCGGGACCCGTCTCCCCCGAGGGCCGGTTGGCGGCAGCCTGGGACGCGCTTATCGTGCGGCCAGTCCGG
  CGCTGGCGCCGCGTGGCAGTGGGAGTCAATGCATGTGTTGATGTGGTGCTCTCAGGGGTGAAGCTCTTGC
  AGGCACTTGGCCTTAGTCCTGGGAATGGGAAAGATCACAGCATTCTGCATTCAAGGAATGATCTGGAAGA
  AGCCTTCATTCACTTCATGGGGAAGGGAGCAGCTGCTGAGCGCTTCTTCAGTGATAAGGAAACTTTTCAC
  GACATTGCCCAGGTTGCGTCAGAGTTCCCAGGAGCCCAGCACTATGTAGGAGGAAATGCAGCTTTAATTG
  GACAGAAATTTGCAGCCAACTCAGATTTAAAGGTTCTTCTTTGCGGTCCAGTTGGTCCAAAGCTACATGA
  GCTTCTTGATGACAATGTCTTTGTTCCACCAGAGTCATTGCAGGAAGTGGATGAGTTCCACCTCATTTTA
  GAGTATCAAGCAGGGGAGGAGTGGGGCCAGTTAAAAGCTCCCCATGCCAACCGATTCATCTTCTCTCACG
  ACCTCTCCAACGGGGCCATGAATATGCTGGAGGTGTTTGTGTCTAGCCTGGAGGAGTTTCAGCCAGACCT
  GGTGGTCCTCTCTGGATTGCACATGATGGAGGGACAAAGCAAGGAGCTCCAGAGGAAGAGACTCTTGGAG
  GTTGTAACCTCCATTTCTGACATCCCCACTGGTATTCCAGTTCACCTAGAGCTGGCCAGTATGACTAACA
  GGGAGCTCATGAGCAGCATTGTCCATCAGCAGGTCTTTCCCGCGGTGACTTCCCTTGGGCTGAATGAACA
  GGAGCTGTTATTTCTCACCCAGTCAGCCTCTGGACCTCACTCTTCTCTCTCTTCCTGGAACGGTGTTCCT
  GATGTGGGCATGGTCAGTGACATCCTCTTCTGGATCTTGAAAGAACATGGGAGGAGTAAAAGCAGAGCCT
  CGGATCTCACCAGGATCCATTTCCACACGCTGGTCTACCACATCCTGGCAACTGTGGATGGACACTGGGC
  CAACCAGCTGGCAGCCGTGGCTGCAGGAGCTCGTGTGGCTGGGACACAGGCCTGCGCCACAGAAACCATA
  GACACCAGCCGAGTGTCTCTGAGGGCACCCCAAGAGTTCATGACTTCCCATTCGGAGGCAGGCTCCAGGA
  TTGTATTAAACCCAAACAAGCCAGTAGTAGAATGGCACAGAGAGGGAATATCCTTCCACTTCACACCAGT
  ATTGGTGTGTAAAGACCCCATTCGAACTGTAGGCCTTGGAGATGCCATTTCAGCCGAAGGACTCTTCTAT
  TCGGAAGTACACCCTCACTAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202965 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MALWRGSAYAGFLALAVGCVFLLEPELPGSALRSLWSSLCLGPAPAPPGPVSPEGRLAAAWDALIVRPVR
  RWRRVAVGVNACVDVVLSGVKLLQALGLSPGNGKDHSILHSRNDLEEAFIHFMGKGAAAERFFSDKETFH
  DIAQVASEFPGAQHYVGGNAALIGQKFAANSDLKVLLCGPVGPKLHELLDDNVFVPPESLQEVDEFHLIL
  EYQAGEEWGQLKAPHANRFIFSHDLSNGAMNMLEVFVSSLEEFQPDLVVLSGLHMMEGQSKELQRKRLLE
  VVTSISDIPTGIPVHLELASMTNRELMSSIVHQQVFPAVTSLGLNEQELLFLTQSASGPHSSLSSWNGVP
  DVGMVSDILFWILKEHGRSKSRASDLTRIHFHTLVYHILATVDGHWANQLAAVAAGARVAGTQACATETI
  DTSRVSLRAPQEFMTSHSEAGSRIVLNPNKPVVEWHREGISFHFTPVLVCKDPIRTVGLGDAISAEGLFY
  SEVHPHY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031284
• ORF Size: 1491 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031284.3, NM_031284.4, NP_112574.3
• RefSeq Size: 2565 bp
• RefSeq ORF: 1491 bp
• Locus ID: 83440
• Domains: ADP_PFK_GK
• MW: 54.1 kDa
• Gene Summary: ADPGK (EC 2.7.1.147) catalyzes the ADP-dependent phosphorylation of glucose to glucose-6-phosphate and may play a role in glycolysis, possibly during ischemic conditions (Ronimus and Morgan, 2004 [PubMed 14975750]). [supplied by OMIM, Mar 2008]