ADPGK (NM_031284) Human Tagged ORF Clone

CAT#: RG202965

ADPGK (GFP-tagged) - Human ADP-dependent glucokinase (ADPGK), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ADPGK
• Synonyms: 2610017G09Rik; ADP-GK
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG202965 representing NM_031284
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCTGTGGCGCGGCTCCGCGTACGCGGGCTTCCTGGCGCTGGCCGTGGGCTGCGTCTTCCTGCTGG
  AGCCAGAGCTGCCAGGCTCGGCGCTGCGCTCTCTCTGGAGCTCGCTGTGTCTGGGGCCCGCGCCTGCGCC
  CCCGGGACCCGTCTCCCCCGAGGGCCGGTTGGCGGCAGCCTGGGACGCGCTTATCGTGCGGCCAGTCCGG
  CGCTGGCGCCGCGTGGCAGTGGGAGTCAATGCATGTGTTGATGTGGTGCTCTCAGGGGTGAAGCTCTTGC
  AGGCACTTGGCCTTAGTCCTGGGAATGGGAAAGATCACAGCATTCTGCATTCAAGGAATGATCTGGAAGA
  AGCCTTCATTCACTTCATGGGGAAGGGAGCAGCTGCTGAGCGCTTCTTCAGTGATAAGGAAACTTTTCAC
  GACATTGCCCAGGTTGCGTCAGAGTTCCCAGGAGCCCAGCACTATGTAGGAGGAAATGCAGCTTTAATTG
  GACAGAAATTTGCAGCCAACTCAGATTTAAAGGTTCTTCTTTGCGGTCCAGTTGGTCCAAAGCTACATGA
  GCTTCTTGATGACAATGTCTTTGTTCCACCAGAGTCATTGCAGGAAGTGGATGAGTTCCACCTCATTTTA
  GAGTATCAAGCAGGGGAGGAGTGGGGCCAGTTAAAAGCTCCCCATGCCAACCGATTCATCTTCTCTCACG
  ACCTCTCCAACGGGGCCATGAATATGCTGGAGGTGTTTGTGTCTAGCCTGGAGGAGTTTCAGCCAGACCT
  GGTGGTCCTCTCTGGATTGCACATGATGGAGGGACAAAGCAAGGAGCTCCAGAGGAAGAGACTCTTGGAG
  GTTGTAACCTCCATTTCTGACATCCCCACTGGTATTCCAGTTCACCTAGAGCTGGCCAGTATGACTAACA
  GGGAGCTCATGAGCAGCATTGTCCATCAGCAGGTCTTTCCCGCGGTGACTTCCCTTGGGCTGAATGAACA
  GGAGCTGTTATTTCTCACCCAGTCAGCCTCTGGACCTCACTCTTCTCTCTCTTCCTGGAACGGTGTTCCT
  GATGTGGGCATGGTCAGTGACATCCTCTTCTGGATCTTGAAAGAACATGGGAGGAGTAAAAGCAGAGCCT
  CGGATCTCACCAGGATCCATTTCCACACGCTGGTCTACCACATCCTGGCAACTGTGGATGGACACTGGGC
  CAACCAGCTGGCAGCCGTGGCTGCAGGAGCTCGTGTGGCTGGGACACAGGCCTGCGCCACAGAAACCATA
  GACACCAGCCGAGTGTCTCTGAGGGCACCCCAAGAGTTCATGACTTCCCATTCGGAGGCAGGCTCCAGGA
  TTGTATTAAACCCAAACAAGCCAGTAGTAGAATGGCACAGAGAGGGAATATCCTTCCACTTCACACCAGT
  ATTGGTGTGTAAAGACCCCATTCGAACTGTAGGCCTTGGAGATGCCATTTCAGCCGAAGGACTCTTCTAT
  TCGGAAGTACACCCTCACTAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG202965 representing NM_031284
  Red=Cloning site Green=Tags(s)
  
  MALWRGSAYAGFLALAVGCVFLLEPELPGSALRSLWSSLCLGPAPAPPGPVSPEGRLAAAWDALIVRPVR
  RWRRVAVGVNACVDVVLSGVKLLQALGLSPGNGKDHSILHSRNDLEEAFIHFMGKGAAAERFFSDKETFH
  DIAQVASEFPGAQHYVGGNAALIGQKFAANSDLKVLLCGPVGPKLHELLDDNVFVPPESLQEVDEFHLIL
  EYQAGEEWGQLKAPHANRFIFSHDLSNGAMNMLEVFVSSLEEFQPDLVVLSGLHMMEGQSKELQRKRLLE
  VVTSISDIPTGIPVHLELASMTNRELMSSIVHQQVFPAVTSLGLNEQELLFLTQSASGPHSSLSSWNGVP
  DVGMVSDILFWILKEHGRSKSRASDLTRIHFHTLVYHILATVDGHWANQLAAVAAGARVAGTQACATETI
  DTSRVSLRAPQEFMTSHSEAGSRIVLNPNKPVVEWHREGISFHFTPVLVCKDPIRTVGLGDAISAEGLFY
  SEVHPHY
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031284
• ORF Size: 1491 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_031284.3, NP_112574.3
• RefSeq Size: 2492
• RefSeq ORF: 1491
• Locus ID: 83440
• Domains: ADP_PFK_GK
• Gene Summary: ADPGK (EC 2.7.1.147) catalyzes the ADP-dependent phosphorylation of glucose to glucose-6-phosphate and may play a role in glycolysis, possibly during ischemic conditions (Ronimus and Morgan, 2004 [PubMed 14975750]). [supplied by OMIM, Mar 2008]