Myosin light chain 3 (MYL3) (NM_000258) Human Tagged ORF Clone
CAT#: RC203122
MYL3 (Myc-DDK-tagged)-Human myosin, light chain 3, alkali, ventricular, skeletal, slow (MYL3)
"NM_000258" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | MYL3 |
Synonyms | CMH8; MLC-lV/sb; MLC1SB; MLC1V; VLC1; VLCl |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC203122 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCCCAAAAAGCCAGAGCCCAAGAAGGATGATGCCAAGGCAGCCCCCAAGGCAGCTCCAGCTCCCG CACCTCCCCCTGAGCCTGAGCGCCCTAAGGAGGTCGAGTTTGATGCTTCCAAGATCAAGATTGAGTTCAC ACCTGAGCAGATTGAAGAGTTCAAGGAAGCCTTCATGCTGTTCGACCGCACACCCAAGTGTGAGATGAAG ATCACCTACGGGCAGTGTGGGGATGTCCTGCGGGCGCTGGGCCAGAACCCCACACAGGCAGAAGTGCTCC GTGTCCTGGGGAAGCCAAGACAGGAAGAGCTCAATACCAAGATGATGGACTTTGAAACTTTCCTGCCTAT GCTCCAGCACATTTCCAAGAACAAGGACACAGGCACCTATGAGGACTTCGTGGAGGGGCTGCGGGTCTTC GACAAGGAGGGCAATGGCACTGTCATGGGTGCTGAGCTTCGCCACGTGCTGGCCACGCTGGGTGAGAGGC TGACAGAAGACGAAGTGGAGAAGTTGATGGCTGGGCAAGAGGACTCCAATGGCTGCATCAACTATGAAGC ATTTGTGAAGCACATCATGTCCAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203122 protein sequence
Red=Cloning site Green=Tags(s) MAPKKPEPKKDDAKAAPKAAPAPAPPPEPERPKEVEFDASKIKIEFTPEQIEEFKEAFMLFDRTPKCEMK ITYGQCGDVLRALGQNPTQAEVLRVLGKPRQEELNTKMMDFETFLPMLQHISKNKDTGTYEDFVEGLRVF DKEGNGTVMGAELRHVLATLGERLTEDEVEKLMAGQEDSNGCINYEAFVKHIMSS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_000258 |
ORF Size | 585 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000258.1, NM_000258.2, NP_000249.1 |
RefSeq Size | 942 bp |
RefSeq ORF | 588 bp |
Locus ID | 4634 |
Cytogenetics | 3p21.31 |
Protein Families | Druggable Genome |
Protein Pathways | Cardiac muscle contraction, Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM) |
MW | 21.9 kDa |
Gene Summary | 'MYL3 encodes myosin light chain 3, an alkali light chain also referred to in the literature as both the ventricular isoform and the slow skeletal muscle isoform. Mutations in MYL3 have been identified as a cause of mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC122554 | MYL3 (untagged)-Human myosin, light chain 3, alkali, ventricular, skeletal, slow (MYL3) |
USD 310.00 |
|
RG203122 | MYL3 (GFP-tagged) - Human myosin, light chain 3, alkali; ventricular, skeletal, slow (MYL3) |
USD 460.00 |
|
RC203122L1 | Lenti ORF clone of Human myosin, light chain 3, alkali; ventricular, skeletal, slow (MYL3), Myc-DDK-tagged |
USD 768.00 |
|
RC203122L2 | Lenti ORF clone of Human myosin, light chain 3, alkali; ventricular, skeletal, slow (MYL3), mGFP tagged |
USD 620.00 |
|
RC203122L3 | Lenti ORF clone of Human myosin, light chain 3, alkali; ventricular, skeletal, slow (MYL3), Myc-DDK-tagged |
USD 620.00 |
|
RC203122L4 | Lenti ORF clone of Human myosin, light chain 3, alkali; ventricular, skeletal, slow (MYL3), mGFP tagged |
USD 620.00 |
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