Tbp7 (PSMC4) (NM_153001) Human Tagged ORF Clone

CAT#: RC203161

PSMC4 (Myc-DDK-tagged)-Human proteasome (prosome, macropain) 26S subunit, ATPase, 4 (PSMC4), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY407188) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203161 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PSMC4
• Synonyms: MIP224; RPT3; S6; TBP-7; TBP7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203161 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAGATAGGCATCTTGGTGGAGAAGGCTCAGGATGAGATCCCAGCACTGTCCGTGTCCCGGCCCC
  AGACCGGCCTGTCCTTCCTGGGCCCTGAGCCTGAGGACCTGGAGGACCTGTACAGCCGCTACAAGGAGGA
  GGTGAAGCGAATCCAAAGCATCCCGCTGGTCATCGGACAATTTCTGGAGGCTGTGGATCAGAATACAGCC
  ATCGTGGGCTCTACCACAGGCTCCAACTATTATGTGCGCATCCTGAGCACCATCGATCGGGAGCTGCTCA
  AGCCCAACGCCTCAGTGGCCCTCCACAAGCACAGCAATGCACTGGTGGACGTGCTGCCCCCCGAAGCCGA
  CAGCAGCATCATGATGCTCACCTCAGACCAGAAGCCAGATGTGATGTACGCGGACATCGGAGGCATGGAC
  ATCCAGAAGCAGGAGGTGCGGGAGGCCGTGGAGCTCCCGCTCACGCATTTCGAGCTCTACAAGCAGATCG
  GCATCGATCCCCCCCGAGGCGTCCTCATGTATGGCCCACCTGGCTGTGGGAAGACCATGTTGGCAAAGGC
  GGTGGCACATCACACAACAGCTGCATTCATCCGGGTCGTGGGCTCGGAGTTTGTACAGAAGTATCTGGGT
  GAGGGCCCCCGCATGGTCCGGGATGTGTTCCGCCTGGCCAAGGAGAATGCACCTGCCATCATCTTCATAG
  ACGAGATTGATGCCATCGCCACCAAGAGATTCGATGCTCAGACAGGGGCCGACAGGGAGGTTCAGAGGAT
  CCTGCTGGAGCTGCTGAATCAGATGGATGGATTTGATCAGAATGTCAATGTCAAGGTAATCATGGCCACA
  AACAGAGCAGACACCCTGGATCCGGCCCTGCTACGGCCAGGACGGCTGGACCGTAAAATTGAATTTCCAC
  TTCCTGACCGCCGCCAGAAGAGATTGATTTTCTCCACTATCACTAGCAAGATGAACCTCTCTGAGGAGGT
  TGACTTGGAAGACTATGTGGCCCGGCCAGATAAGATTTCAGGAGCTGATATTAACTCCATCTGTCAGGAG
  AGTGGAATGTTGGCTGTCCGTGAAAACCGCTACATTGTCCTGGCCAAGGACTTCGAGAAAGCATACAAGA
  CTGTCATCAAGAAGGACGAGCAGGAGCATGAGTTTTACAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203161 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEEIGILVEKAQDEIPALSVSRPQTGLSFLGPEPEDLEDLYSRYKEEVKRIQSIPLVIGQFLEAVDQNTA
  IVGSTTGSNYYVRILSTIDRELLKPNASVALHKHSNALVDVLPPEADSSIMMLTSDQKPDVMYADIGGMD
  IQKQEVREAVELPLTHFELYKQIGIDPPRGVLMYGPPGCGKTMLAKAVAHHTTAAFIRVVGSEFVQKYLG
  EGPRMVRDVFRLAKENAPAIIFIDEIDAIATKRFDAQTGADREVQRILLELLNQMDGFDQNVNVKVIMAT
  NRADTLDPALLRPGRLDRKIEFPLPDRRQKRLIFSTITSKMNLSEEVDLEDYVARPDKISGADINSICQE
  SGMLAVRENRYIVLAKDFEKAYKTVIKKDEQEHEFYK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153001
• ORF Size: 1161 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153001.1, NM_153001.2, NP_694546.1
• RefSeq Size: 1821 bp
• RefSeq ORF: 1164 bp
• Locus ID: 5704
• Cytogenetics: 19q13.2
• Domains: AAA, AAA
• Protein Families: Druggable Genome
• Protein Pathways: Proteasome
• MW: 43.5 kDa
• Gene Summary: 'The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a member of the triple-A family of ATPases that is a component of the 19S regulatory subunit and plays a role in 26S proteasome assembly. The encoded protein interacts with gankyrin, a liver oncoprotein, and may also play a role in Parkinson's disease through interactions with synphilin-1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]'