Tbp7 (PSMC4) (NM_153001) Human Untagged Clone

CAT#: SC109643

PSMC4 (untagged)-Human proteasome (prosome, macropain) 26S subunit, ATPase, 4 (PSMC4), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PSMC4
• Synonyms: MIP224; RPT3; S6; TBP-7; TBP7
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_153001, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGGAGATAGGCATCTTGGTGGAGAAGGCTCAGGATGAGATCCCAGCACTGTCCGTGTCCCGGCCCC
  AGACCGGCCTGTCCTTCCTGGGCCCTGAGCCTGAGGACCTGGAGGACCTGTACAGCCGCTACAAGGAGGA
  GGTGAAGCGAATCCAAAGCATCCCGCTGGTCATCGGACAATTTCTGGAGGCTGTGGATCAGAATACAGCC
  ATCGTGGGCTCTACCACAGGCTCCAACTATTATGTGCGCATCCTGAGCACCATCGATCGGGAGCTGCTCA
  AGCCCAACGCCTCAGTGGCCCTCCACAAGCACAGCAATGCACTGGTGGACGTGCTGCCCCCCGAAGCCGA
  CAGCAGCATCATGATGCTCACCTCAGACCAGAAGCCAGATGTGATGTACGCGGACATCGGAGGCATGGAC
  ATCCAGAAGCAGGAGGTGCGGGAGGCCGTGGAGCTCCCGCTCACGCATTTCGAGCTCTACAAGCAGATCG
  GCATCGATCCCCCCCGAGGCGTCCTCATGTATGGCCCACCTGGCTGTGGGAAGACCATGTTGGCAAAGGC
  GGTGGCACATCACACAACAGCTGCATTCATCCGGGTCGTGGGCTCGGAGTTTGTACAGAAGTATCTGGGT
  GAGGGCCCCCGCATGGTCCGGGATGTGTTCCGCCTGGCCAAGGAGAATGCACCTGCCATCATCTTCATAG
  ACGAGATTGATGCCATCGCCACCAAGAGATTCGATGCTCAGACAGGGGCCGACAGGGAGGTTCAGAGGAT
  CCTGCTGGAGCTGCTGAATCAGATGGATGGATTTGATCAGAATGTCAATGTCAAGGTAATCATGGCCACA
  AACAGAGCAGACACCCTGGATCCGGCCCTGCTACGGCCAGGACGGCTGGACCGTAAAATTGAATTTCCAC
  TTCCTGACCGCCGCCAGAAGAGATTGATTTTCTCCACTATCACTAGCAAGATGAACCTCTCTGAGGAGGT
  TGACTTGGAAGACTATGTGGCCCGGCCAGATAAGATTTCAGGAGCTGATATTAACTCCATCTGTCAGGAG
  AGTGGAATGTTGGCTGTCCGTGAAAACCGCTACATTGTCCTGGCCAAGGACTTCGAGAAAGCATACAAGA
  CTGTCATCAAGAAGGACGAGCAGGAGCATGAGTTTTACAAGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_153001 unedited
  ACACGACTACTATAGGGCGGCCGCCAATTCGGCACGAGGCAGAGGCCGTCTTGGTCACTA
  TGGAGGAGATAGGCATCTTGGTGGAGAAGGCTCAGGATGAGATCCCAGCACTGTCCGTGT
  CCCGGCCCCAGACCGGCCTGTCCTTCCTGGGCCCTGAGCCTGAGGACCTGGAGGACCTGT
  ACAGCCGCTACAAGAAGCTGCAGCAAGAGCTGGAGTTCCTGGAGGTGCAGGAGGAATACA
  TCAAAGATGAGCAAAAGAACCTGAAAAAGGAATTTCTCCATGCCTAGGAGGAGGTGAAGC
  GAATCCAAAGCATCCCGCTGGTCATCGGACAATTTCTGGAGGCTGTGGATCAGAATACAG
  CCATCGTGGGCTCTACCACAGGCTCCAACTATTATGTGCGCATCCTGAGCACCATCGATC
  GGGAGCTGCTCAAGCCCAACGCCTCAGTGGCCCTCCACAAGCACAGCAATGCACTGGTGG
  ACGTGCTGCCCCCCGAAGCCGACAGCAGCATCATGATGCTCACCTCAGACCAGAAGCCAG
  ATGTGATGTACGCGGACATCGGAGGCATGGACATCCATAAGCAGGAGGTGCGGGAGGCCG
  TGGAGCTCCCGCTCACGCATTTCGAGCTCTACAAGCAGATCGGCATCGATCCCCCCCGAG
  GCGTCCTCATGTATGGCCCACCTGGCTGTGGGATGACCATGTGGCAAGGCGGTGCACATC
  ACCAACAGCTGCATCATCCGNGTCGTGGGCTCGGAGTTGTACAGAGTATCTGGGTGAGGG
  CCCCGCATGGTCCGGATGTGTCCGCCTGGCCAGGAGATGCCCTGCCTCATCTNATAGACA
  GATGATGCATCGCACCAGAGATCCAGCTAAACAGGCCGACAGGAGGTCANAGATCTGCCG
  AGCGCTGATANATGATGATTGACAAATGCATGTCAGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_153001
• Insert Size: 1500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153001.1, NP_694546.1
• RefSeq Size: 1363 bp
• RefSeq ORF: 1164 bp
• Locus ID: 5704
• Cytogenetics: 19q13.2
• Domains: AAA, AAA
• Protein Families: Druggable Genome
• Protein Pathways: Proteasome
• Gene Summary: 'The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a member of the triple-A family of ATPases that is a component of the 19S regulatory subunit and plays a role in 26S proteasome assembly. The encoded protein interacts with gankyrin, a liver oncoprotein, and may also play a role in Parkinson's disease through interactions with synphilin-1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]'
  Transcript Variant: This variant (2) uses an alternate splice site in the 5' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.