ALS2CR2 (STRADB) (NM_018571) Human Tagged ORF Clone

CAT#: RC203432

STRADB (Myc-DDK-tagged)-Human STE20-related kinase adaptor beta (STRADB), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY402696) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203432 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: STRADB
• Synonyms: ALS2CR2; CALS-21; ILPIP; ILPIPA; PAPK; PRO1038
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203432 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCTTTTGGATTGCTTCTGCACTTCAAGAACACAAGTTGAATCACTCAGACCTGAAAAACAGTCTG
  AAACCAGTATCCATCAATACTTGGTTGATGAGCCAACCCTTTCCTGGTCACGTCCATCCACTAGAGCCAG
  TGAAGTACTATGTTCCACCAACGTTTCTCACTATGAGCTCCAAGTAGAAATAGGAAGAGGATTTGACAAC
  TTGACTTCTGTCCATCTTGCACGGCATACTCCCACGGGAACACTGGTAACTATAAAAATTACAAATCTGG
  AAAACTGCAATGAAGAACGCCTGAAAGCTTTACAGAAAGCCGTGATTCTATCCCACTTTTTCCGGCATCC
  CAATATTACAACTTATTGGACAGTTTTCACTGTTGGCAGCTGGCTTTGGGTTATTTCTCCATTTATGGCC
  TATGGTTCAGCAAGTCAACTCTTGAGGACCTATTTTCCTGAAGGAATGAGTGAAACTTTAATAAGAAACA
  TTCTCTTTGGAGCCGTGAGAGGGTTGAACTATCTGCACCAAAATGGCTGTATTCACAGGAGTATTAAAGC
  CAGCCATATCCTCATTTCTGGTGATGGCCTAGTGACCCTCTCTGGCCTTTCCCATCTGCATAGTTTGGTT
  AAGCATGGACAGAGGCATAGGGCTGTGTATGATTTCCCACAGTTCAGCACATCAGTGCAGCCGTGGCTGA
  GTCCAGAACTACTGAGACAGGATTTACATGGGTATAATGTGAAGTCAGATATTTACAGTGTTGGGATTAC
  AGCATGTGAATTAGCCAGTGGGCAGGTGCCTTTCCAGGACATGCATAGAACTCAGATGCTGTTACAGAAA
  CTGAAAGGTCCTCCTTATAGCCCATTGGATATCAGTATTTTCCCTCAATCAGAATCCAGAATGAAAAATT
  CCCAGTCAGGTGTAGACTCTGGGATTGGAGAAAGTGTGCTTGTCTCCAGTGGAACTCACACAGTAAATAG
  TGACCGATTACACACACCATCCTCAAAAACTTTCTCTCCTGCCTTCTTTAGCTTGGTACAGCTCTGTTTG
  CAACAAGATCCTGAGAAAAGGCCATCAGCAAGCAGTTTATTGTCCCATGTTTTCTTCAAACAGATGAAAG
  AAGAAAGCCAGGATTCAATACTTTCACTGTTGCCTCCTGCTTATAACAAGCCATCAATATCATTGCCTCC
  AGTGTTACCTTGGACTGAGCCAGAATGTGATTTTCCTGATGAAAAAGACTCATACTGGGAATTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203432 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSLLDCFCTSRTQVESLRPEKQSETSIHQYLVDEPTLSWSRPSTRASEVLCSTNVSHYELQVEIGRGFDN
  LTSVHLARHTPTGTLVTIKITNLENCNEERLKALQKAVILSHFFRHPNITTYWTVFTVGSWLWVISPFMA
  YGSASQLLRTYFPEGMSETLIRNILFGAVRGLNYLHQNGCIHRSIKASHILISGDGLVTLSGLSHLHSLV
  KHGQRHRAVYDFPQFSTSVQPWLSPELLRQDLHGYNVKSDIYSVGITACELASGQVPFQDMHRTQMLLQK
  LKGPPYSPLDISIFPQSESRMKNSQSGVDSGIGESVLVSSGTHTVNSDRLHTPSSKTFSPAFFSLVQLCL
  QQDPEKRPSASSLLSHVFFKQMKEESQDSILSLLPPAYNKPSISLPPVLPWTEPECDFPDEKDSYWEF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018571
• ORF Size: 1254 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018571.2, NM_018571.3, NM_018571.4, NM_018571.5, NP_061041.2
• RefSeq Size: 2300 bp
• RefSeq ORF: 1257 bp
• Locus ID: 55437
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• MW: 47 kDa
• Gene Summary: This gene encodes a protein that belongs to the serine/threonine protein kinase STE20 subfamily. One of the active site residues in the protein kinase domain of this protein is altered, and it is thus a pseudokinase. This protein is a component of a complex involved in the activation of serine/threonine kinase 11, a master kinase that regulates cell polarity and energy-generating metabolism. This complex regulates the relocation of this kinase from the nucleus to the cytoplasm, and it is essential for G1 cell cycle arrest mediated by this kinase. The protein encoded by this gene can also interact with the X chromosome-linked inhibitor of apoptosis protein, and this interaction enhances the anti-apoptotic activity of this protein via the JNK1 signal transduction pathway. Two pseudogenes, located on chromosomes 1 and 7, have been found for this gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2011]