BANP (NM_017869) Human Tagged ORF Clone

CAT#: RC203448

BANP (Myc-DDK-tagged)-Human BTG3 associated nuclear protein (BANP), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY409206) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC212927 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BANP
• Synonyms: BEND1; SMAR1; SMARBP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203448 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGTCGGAACACGACCTGGCCGATGTGGTTCAGATTGCAGTGGAAGACCTGAGCCCTGACCACCCAG
  TTGTTTTGGAGAATCATGTAGTGACAGATGAAGACGAACCTGCTTTGAAACGCCAGCGACTAGAAATCAA
  TTGCCAGGATCCATCTATAAAGTCATTCCTGTATTCCATCAACCAGACAATCTGCTTGCGGTTGGATAGC
  ATTGAAGCCAAATTGCAAGCCCTGGAGGCTACTTGTAAATCCTTAGAAGAAAAGCTGGATCTGGTCACGA
  ACAAGCAGCACAGCCCCATCCAGGTCCCCATGGTGGCCGGCTCCCCTCTCGGGGCAACCCAGACGTGCAA
  CAAAGTGCGATGCGCTGTGCCTGGGCGTCGGCAGAACACCATTGTGGTGAAGGTGCCGGGCCAAGAAGAC
  AGCCACCACGAGGACGGGGAGAGCGGCTCGGAGGCCAGCGACTCTGTGTCCAGCTGTGGGCAGGCGGGCA
  GTCAGAGCATCGGGAGCAACGTCACGCTCATCACCCTGAACTCGGAAGAGGACTACCCCAATGGCACCTG
  GCTGGGCGACGAGAACAACCCCGAGATGCGGGTACGCTGCGCCATCATCCCCTCCGACATGCTGCACATC
  AGCACCAACTGCCGCACGGCTGAGAAGATGGCGCTAACGCTGCTGGACTACCTCTTCCACCGCGAGGTGC
  AGGCTGTGTCCAACCTCTCGGGGCAGGGCAAGCACGGGAAGAAGCAGCTGGACCCGCTCACCATCTACGG
  CATCCGGTGTCACCTTTTCTATAAATTTGGCATCACAGAATCCGACTGGTACCGAATCAAGCAGAGCATC
  GACTCCAAGTGCCGCACGGCGTGGCGGCGCAAGCAGCGGGGCCAGAGCCTGGCGGTCAAGAGCTTCTCGC
  GGAGAACGCCCAACTCGTCCTCCTACTGCCCTTCAGAGCCGATGATGAGCACCCCACCTCCTGCCAGCGA
  GCTCCCGCAGCCACAGCCGCAGCCGCAGGCCCTGCACTACGCGCTGGCCAACGCACAGCAGGTGCAGATC
  CACCAGATCGGAGAAGACGGACAGGTGCAAGTAATCCCACAGGGACACCTCCACATCGCCCAGGTGCCGC
  AGGGGGAGCAAGTCCAGATCACGCAGGACAGCGAGGGCAACCTCCAGATCCATCACGTGGGGCAGGACGG
  TCAGGTGCTGCAGGGTGCACAGCTGATCGCCGTGGCCTCCTCGGACCCCGCGGCGGCGGGCGTGGATGGG
  TCGCCACTCCAGGGCAGCGACATCCAGGTTCAGTACGTGCAGCTGGCGCCAGTGAGTGACCACACGGCCG
  GGGCACAGACGGCCGAAGCCCTGCAGCCCACGCTACAGCCGGAGATGCAGCTCGAGCACGGGGCCATCCA
  GATTCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203448 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MMSEHDLADVVQIAVEDLSPDHPVVLENHVVTDEDEPALKRQRLEINCQDPSIKSFLYSINQTICLRLDS
  IEAKLQALEATCKSLEEKLDLVTNKQHSPIQVPMVAGSPLGATQTCNKVRCAVPGRRQNTIVVKVPGQED
  SHHEDGESGSEASDSVSSCGQAGSQSIGSNVTLITLNSEEDYPNGTWLGDENNPEMRVRCAIIPSDMLHI
  STNCRTAEKMALTLLDYLFHREVQAVSNLSGQGKHGKKQLDPLTIYGIRCHLFYKFGITESDWYRIKQSI
  DSKCRTAWRRKQRGQSLAVKSFSRRTPNSSSYCPSEPMMSTPPPASELPQPQPQPQALHYALANAQQVQI
  HQIGEDGQVQVIPQGHLHIAQVPQGEQVQITQDSEGNLQIHHVGQDGQVLQGAQLIAVASSDPAAAGVDG
  SPLQGSDIQVQYVQLAPVSDHTAGAQTAEALQPTLQPEMQLEHGAIQIQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017869
• ORF Size: 1407 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 2230 bp
• RefSeq ORF: 1410 bp
• Locus ID: 54971
• MW: 51.2 kDa
• Gene Summary: This gene encodes a protein that binds to matrix attachment regions. The protein forms a complex with p53 and negatively regulates p53 transcription, and functions as a tumor suppressor and cell cycle regulator. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]