NDUFA9 (NM_005002) Human Tagged ORF Clone

CAT#: RC203464

NDUFA9 (Myc-DDK-tagged)-Human NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 9, 39kDa (NDUFA9), nuclear gene encoding mitochondrial protein

Product Images

Western blot validation of overexpression lysate (Cat# LY401557) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203464 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified NDUFA9 protein (Cat# TP303464). The protein was produced from HEK293T cells transfected with NDUFA9 cDNA clone (Cat# RC203464) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NDUFA9
• Synonyms: CC6; CI-39k; CI39k; COQ11; MC1DN26; NDUFS2L; SDR22E1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203464 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCTGCCGCACAATCCCGGGTTGTCCGGGTCCTGTCAATGTCACGTTCTGCCATTACTGCAATAG
  CCACATCTGTGTGTCACGGCCCACCCTGTCGCCAGCTTCATCATGCCCTCATGCCTCATGGGAAAGGTGG
  ACGTTCCTCAGTCAGTGGGATTGTGGCCACTGTGTTTGGAGCAACAGGATTCCTGGGGCGATATGTTGTC
  AACCACCTTGGACGCATGGGGTCACAGGTAATCATACCCTATCGGTGTGATAAATATGACATCATGCACC
  TTCGTCCCATGGGTGACCTGGGCCAGCTTCTGTTTCTGGAATGGGACGCGAGAGATAAAGATTCTATCCG
  ACGAGTAGTACAACACAGCAATGTGGTCATCAATCTTATTGGACGAGACTGGGAAACCAAAAACTTTGAT
  TTTGAGGATGTTTTTGTGAAGATTCCCCAAGCAATTGCTCAACTGTCCAAGGAAGCTGGAGTTGAAAAAT
  TCATTCATGTTTCACATCTGAATGCGAATATTAAAAGCTCTTCTAGATATTTGAGAAATAAGGCTGTTGG
  AGAGAAAGTAGTGAGAGATGCATTTCCGGAAGCCATTATCGTAAAGCCGTCGGACATCTTTGGAAGAGAG
  GATAGATTCCTTAATTCTTTTGCAAGTATGCATCGGTTTGGTCCTATACCCCTTGGTTCCTTGGGCTGGA
  AGACAGTTAAACAACCAGTATATGTCGTAGATGTATCCAAAGGAATTGTTAATGCAGTTAAGGATCCTGA
  TGCCAATGGGAAATCCTTTGCTTTCGTTGGTCCCAGTCGGTACCTCCTTTTCCACCTGGTGAAGTACATC
  TTTGCTGTGGCTCACAGATTGTTCCTCCCATTCCCCTTGCCGCTTTTTGCCTATCGATGGGTAGCAAGAG
  TCTTTGAAATAAGCCCATTTGAGCCCTGGATAACAAGGGATAAAGTGGAGCGGATGCACATCACAGACAT
  GAAATTGCCTCACCTGCCTGGCTTAGAAGACCTTGGTATTCAGGCAACACCACTGGAACTCAAGGCCATT
  GAGGTGCTGCGGCGTCATCGCACTTACCGCTGGCTGTCTGCTGAAATTGAGGATGTGAAGCCGGCCAAGA
  CCGTCAACATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203464 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAAAQSRVVRVLSMSRSAITAIATSVCHGPPCRQLHHALMPHGKGGRSSVSGIVATVFGATGFLGRYVV
  NHLGRMGSQVIIPYRCDKYDIMHLRPMGDLGQLLFLEWDARDKDSIRRVVQHSNVVINLIGRDWETKNFD
  FEDVFVKIPQAIAQLSKEAGVEKFIHVSHLNANIKSSSRYLRNKAVGEKVVRDAFPEAIIVKPSDIFGRE
  DRFLNSFASMHRFGPIPLGSLGWKTVKQPVYVVDVSKGIVNAVKDPDANGKSFAFVGPSRYLLFHLVKYI
  FAVAHRLFLPFPLPLFAYRWVARVFEISPFEPWITRDKVERMHITDMKLPHLPGLEDLGIQATPLELKAI
  EVLRRHRTYRWLSAEIEDVKPAKTVNI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005002
• ORF Size: 1131 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005002.1, NM_005002.2, NM_005002.3, NM_005002.4, NP_004993.1
• RefSeq Size: 1621 bp
• RefSeq ORF: 1134 bp
• Locus ID: 4704
• Cytogenetics: 12p13.32
• Protein Pathways: Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
• MW: 42.5 kDa
• Gene Summary: 'The encoded protein is a subunit of the hydrophobic protein fraction of the NADH:ubiquinone oxidoreductase (complex I), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane. A pseudogene has been identified on chromosome 12. [provided by RefSeq, May 2010]'