ENTPD6 (NM_001247) Human Tagged ORF Clone

CAT#: RC203487

ENTPD6 (Myc-DDK-tagged)-Human ectonucleoside triphosphate diphosphohydrolase 6 (putative) (ENTPD6), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY400498) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203487 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified ENTPD6 protein (Cat# TP303487). The protein was produced from HEK293T cells transfected with ENTPD6 cDNA clone (Cat# RC203487) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ENTPD6
• Synonyms: CD39L2; dJ738P15.3; IL-6SAG; IL6ST2; NTPDase-6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203487 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAAAAGGTATCCGTTATGAAACTTCCAGAAAAACGAGCTACATTTTTCAGCCGCAGCACGGTCCTT
  GGCAAACAAGGATGAGAAAAATATCCAACCACGGGAGCCTGCGGGTGGCGAAGGTGGCATACCCCCTGGG
  GCTGTGTGTGGGCGTGTTCATCTATGTTGCCTACATCAAGTGGCACCGGGCCACCGCCACCCAGGCCTTC
  TTCAGCATCACCAGGGCAGCCCCGGGGGCCCGGTGGGGTCAGCAGGCCCACAGCCCCCTGGGGACAGCTG
  CAGACGGGCACGAGGTCTTCTACGGGATCATGTTTGATGCAGGAAGCACTGGCACCCGAGTACACGTCTT
  CCAGTTCACCCGGCCCCCCAGAGAAACTCCCACGTTAACCCACGAAACCTTCAAAGCACTGAAGCCAGGT
  CTTTCTGCCTATGCTGATGATGTTGAAAAGAGCGCTCAGGGAATCCGGGAACTACTGGATGTTGCTAAAC
  AGGACATTCCGTTCGACTTCTGGAAGGCCACCCCTCTGGTCCTCAAGGCCACAGCTGGCTTACGCCTGTT
  ACCTGGAGAAAAGGCCCAGAAGTTACTGCAGAAGGTGAAAGAAGTATTTAAAGCATCGCCTTTCCTTGTA
  GGGGATGACTGTGTTTCCATCATGAACGGAACAGATGAAGGCGTTTCGGCGTGGATCACCATCAACTTCC
  TGACAGGCAGCTTGAAAACTCCAGGAGGGAGCAGCGTGGGCATGCTGGACTTGGGCGGAGGATCCACTCA
  GATCGCCTTCCTGCCACGCGTGGAGGGCACCCTGCAGGCCTCCCCACCCGGCTACCTGACGGCACTGCGG
  ATGTTTAACAGGACCTACAAGCTCTATTCTTACAGCTACCTCGGGCTCGGGCTGATGTCGGCACGCCTGG
  CGATCCTGGGCGGCGTGGAGGGGCAGCCTGCTAAGGATGGAAAGGAGTTGGTCAGCCCTTGCTTGTCTCC
  CAGTTTCAAAGGAGAGTGGGAACACGCAGAAGTCACGTACAGGGTTTCAGGGCAGAAAGCAGCGGCAAGC
  CTGCACGAGCTGTGTGCTGCCAGAGTGTCAGAGGTCCTTCAAAACAGAGTGCACAGGACGGAGGAAGTGA
  AGCATGTGGACTTCTATGCTTTCTCCTACTATTACGACCTTGCAGCTGGTGTGGGCCTCATAGATGCGGA
  GAAGGGAGGCAGCCTGGTGGTGGGGGACTTCGAGATCGCAGCCAAGTACGTGTGTCGGACCCTGGAGACA
  CAGCCGCAGAGCAGCCCCTTCTCATGCATGGACCTCACCTACGTCAGCCTGCTACTCCAGGAGTTCGGCT
  TTCCCAGGAGCAAAGTGCTGAAGCTCACTCGGAAAATTGACAATGTTGAGACCAGCTGGGCTCTGGGGGC
  CATTTTTCATTACATCGACTCCCTGAACAGACAGAAGAGTCCAGCCTCA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203487 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKKGIRYETSRKTSYIFQPQHGPWQTRMRKISNHGSLRVAKVAYPLGLCVGVFIYVAYIKWHRATATQAF
  FSITRAAPGARWGQQAHSPLGTAADGHEVFYGIMFDAGSTGTRVHVFQFTRPPRETPTLTHETFKALKPG
  LSAYADDVEKSAQGIRELLDVAKQDIPFDFWKATPLVLKATAGLRLLPGEKAQKLLQKVKEVFKASPFLV
  GDDCVSIMNGTDEGVSAWITINFLTGSLKTPGGSSVGMLDLGGGSTQIAFLPRVEGTLQASPPGYLTALR
  MFNRTYKLYSYSYLGLGLMSARLAILGGVEGQPAKDGKELVSPCLSPSFKGEWEHAEVTYRVSGQKAAAS
  LHELCAARVSEVLQNRVHRTEEVKHVDFYAFSYYYDLAAGVGLIDAEKGGSLVVGDFEIAAKYVCRTLET
  QPQSSPFSCMDLTYVSLLLQEFGFPRSKVLKLTRKIDNVETSWALGAIFHYIDSLNRQKSPAS
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001247
• ORF Size: 2118 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001247.1, NM_001247.2, NM_001247.3, NM_001247.4, NP_001238.1
• RefSeq Size: 2787 bp
• RefSeq ORF: 1455 bp
• Locus ID: 955
• Cytogenetics: 20p11.21
• Domains: GDA1_CD39
• Protein Families: Secreted Protein, Transmembrane
• Protein Pathways: Purine metabolism, Pyrimidine metabolism
• MW: 53.1 kDa
• Gene Summary: 'ENTPD6 is similar to E-type nucleotidases (NTPases). NTPases, such as CD39, mediate catabolism of extracellular nucleotides. ENTPD6 contains 4 apyrase-conserved regions which are characteristic of NTPases. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]'