Sts1 (UBASH3B) (NM_032873) Human Tagged ORF Clone
CAT#: RC203523
UBASH3B (Myc-DDK-tagged)-Human ubiquitin associated and SH3 domain containing B (UBASH3B)
"NM_032873" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | UBASH3B |
Synonyms | p70; STS-1; STS1; TULA-2; TULA2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC203523 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCAGTACGGCCACCCCAGTCCGCTCGGCATGGCTGCGAGAGAGGAGCTGTACAGCAAAGTCACCC CCCGGAGGAACCGCCAACAGCGCCCCGGCACCATCAAGCATGGATCGGCGCTGGACGTGCTCCTCTCCAT GGGGTTCCCCAGAGCCCGCGCACAAAAAGCCTTGGCATCCACGGGAGGAAGAAGTGTTCAGGCAGCATGT GACTGGTTATTCTCCCATGTCGGTGACCCCTTCCTGGATGACCCCCTGCCCCGGGAGTACGTCCTCTACC TCCGTCCCACCGGCCCCTTAGCACAGAAGCTTTCCGACTTTTGGCAGCAGTCGAAGCAGATCTGCGGGAA GAACAAGGCACACAACATCTTCCCCCACATCACACTCTGCCAGTTCTTTATGTGCGAGGACAGCAAGGTG GATGCCCTGGGGGAAGCCCTGCAGACCACGGTCAGTCGCTGGAAATGTAAGTTCTCGGCCCCGCTGCCCC TGGAGCTCTATACGTCGTCCAACTTCATCGGCCTCTTTGTAAAGGAAGACAGTGCGGAGGTCCTCAAGAA GTTTGCTGCTGACTTTGCTGCAGAGGCTGCATCCAAAACCGAAGTGCATGTGGAACCTCATAAGAAGCAG CTACATGTGACCCTGGCTTACCACTTCCAAGCCAGCCACCTACCCACCCTAGAGAAACTGGCCCAGAACA TTGACGTCAAGCTAGGGTGTGACTGGGTGGCTACCATATTTTCTCGGGATATCCGATTTGCTAACCATGA GACATTACAGGTCATCTACCCCTATACCCCACAAAATGACGATGAGCTGGAGCTGGTCCCCGGGGACTTC ATCTTCATGTCTCCAATGGAGCAGACCAGCACCAGCGAGGGTTGGATCTATGGCACGTCCTTAACCACCG GCTGCTCTGGACTCCTGCCTGAGAATTACATTACCAAGGCTGATGAATGCAGCACCTGGATATTTCATGG TTCTTATTCAATCTTAAATACATCGTCATCCAACTCTCTCACGTTTGGGGATGGAGTATTGGAGAGGCGG CCTTATGAGGACCAGGGGCTCGGGGAGACGACTCCTCTTACTATCATCTGCCAGCCCATGCAGCCGCTGA GGGTCAACAGCCAGCCCGGCCCCCAGAAGCGATGCCTTTTTGTGTGTCGGCATGGTGAGAGGATGGATGT TGTGTTTGGGAAGTACTGGCTGTCCCAGTGCTTCGATGCCAAAGGCCGCTACATACGCACCAACCTGAAC ATGCCTCATAGTTTACCTCAGCGGAGTGGTGGTTTCCGAGATTACGAGAAAGATGCTCCCATCACTGTGT TTGGATGCATGCAAGCAAGACTAGTGGGTGAAGCCTTATTAGAGAGCAATACCATTATCGATCATGTCTA TTGCTCCCCGTCCCTTCGCTGCGTTCAGACTGCACACAATATCTTGAAAGGTTTACAACAAGAAAATCAC TTGAAGATCCGTGTAGAGCCCGGCTTATTTGAGTGGACAAAATGGGTTGCTGGGAGCACATTACCTGCAT GGATACCTCCATCAGAGTTAGCTGCAGCCAACCTGAGTGTTGATACAACCTACAGACCTCACATTCCAAT CAGCAAATTAGTTGTTTCAGAATCCTATGATACTTATATCAGTAGAAGTTTCCAAGTAACAAAAGAAATA ATAAGTGAATGTAAAAGTAAAGGAAATAACATCCTGATTGTGGCCCACGCATCTTCCCTTGAAGCGTGTA CCTGCCAACTTCAGGGCCTGTCACCTCAGAACTCCAAGGACTTCGTACAAATGGTCCGAAAGATCCCATA TCTGGGATTTTGTTCCTGTGAAGAATTAGGAGAAACTGGAATATGGCAGCTGACAGATCCACCAATCCTT CCTCTTACCCATGGACCAACTGGGGGCTTCAACTGGAGAGAGACCTTGCTTCAAGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203523 protein sequence
Red=Cloning site Green=Tags(s) MAQYGHPSPLGMAAREELYSKVTPRRNRQQRPGTIKHGSALDVLLSMGFPRARAQKALASTGGRSVQAAC DWLFSHVGDPFLDDPLPREYVLYLRPTGPLAQKLSDFWQQSKQICGKNKAHNIFPHITLCQFFMCEDSKV DALGEALQTTVSRWKCKFSAPLPLELYTSSNFIGLFVKEDSAEVLKKFAADFAAEAASKTEVHVEPHKKQ LHVTLAYHFQASHLPTLEKLAQNIDVKLGCDWVATIFSRDIRFANHETLQVIYPYTPQNDDELELVPGDF IFMSPMEQTSTSEGWIYGTSLTTGCSGLLPENYITKADECSTWIFHGSYSILNTSSSNSLTFGDGVLERR PYEDQGLGETTPLTIICQPMQPLRVNSQPGPQKRCLFVCRHGERMDVVFGKYWLSQCFDAKGRYIRTNLN MPHSLPQRSGGFRDYEKDAPITVFGCMQARLVGEALLESNTIIDHVYCSPSLRCVQTAHNILKGLQQENH LKIRVEPGLFEWTKWVAGSTLPAWIPPSELAAANLSVDTTYRPHIPISKLVVSESYDTYISRSFQVTKEI ISECKSKGNNILIVAHASSLEACTCQLQGLSPQNSKDFVQMVRKIPYLGFCSCEELGETGIWQLTDPPIL PLTHGPTGGFNWRETLLQE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_032873 |
ORF Size | 1947 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_032873.2, NM_032873.3, NM_032873.4, NP_116262.2 |
RefSeq Size | 6917 bp |
RefSeq ORF | 1950 bp |
Locus ID | 84959 |
Domains | UBA, PGAM, SH3 |
Protein Families | Druggable Genome, ES Cell Differentiation/IPS |
MW | 72.7 kDa |
Gene Summary | This gene encodes a protein that contains a ubiquitin associated domain at the N-terminus, an SH3 domain, and a C-terminal domain with similarities to the catalytic motif of phosphoglycerate mutase. The encoded protein was found to inhibit endocytosis of epidermal growth factor receptor (EGFR) and platelet-derived growth factor receptor. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC319198 | UBASH3B (untagged)-Human ubiquitin associated and SH3 domain containing B (UBASH3B) |
USD 310.00 |
|
RG203523 | UBASH3B (GFP-tagged) - Human ubiquitin associated and SH3 domain containing B (UBASH3B) |
USD 460.00 |
|
RC203523L3 | Lenti ORF clone of Human ubiquitin associated and SH3 domain containing B (UBASH3B), Myc-DDK-tagged |
USD 620.00 |
|
RC203523L4 | Lenti ORF clone of Human ubiquitin associated and SH3 domain containing B (UBASH3B), mGFP tagged |
USD 620.00 |
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