VASP (NM_003370) Human Tagged ORF Clone
CAT#: RC203544L4
- LentiORF®
Lenti-ORF clone of VASP (mGFP-tagged)-Human vasodilator-stimulated phosphoprotein (VASP)
"NM_003370" in other vectors (6)
Product Images
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | mGFP |
Symbol | VASP |
Synonyms | vasodilator-stimulated phosphoprotein |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC203544).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_003370 |
ORF Size | 1140 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_003370.3, NP_003361.1 |
RefSeq Size | 2298 bp |
RefSeq ORF | 1143 bp |
Locus ID | 7408 |
Cytogenetics | 19q13.32 |
Protein Families | Druggable Genome, Stem cell - Pluripotency |
Protein Pathways | Fc gamma R-mediated phagocytosis, Focal adhesion, Leukocyte transendothelial migration |
MW | 40.3 kDa |
Gene Summary | 'Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. [provided by RefSeq, Jul 2008]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC122656 | VASP (untagged)-Human vasodilator-stimulated phosphoprotein (VASP) |
USD 310.00 |
|
RC203544 | VASP (Myc-DDK-tagged)-Human vasodilator-stimulated phosphoprotein (VASP) |
USD 420.00 |
|
RG203544 | VASP (GFP-tagged) - Human vasodilator-stimulated phosphoprotein (VASP) |
USD 460.00 |
|
RC203544L1 | Lenti-ORF clone of VASP (Myc-DDK-tagged)-Human vasodilator-stimulated phosphoprotein (VASP) |
USD 768.00 |
|
RC203544L2 | Lenti-ORF clone of VASP (mGFP-tagged)-Human vasodilator-stimulated phosphoprotein (VASP) |
USD 620.00 |
|
RC203544L3 | Lenti-ORF clone of VASP (Myc-DDK-tagged)-Human vasodilator-stimulated phosphoprotein (VASP) |
USD 620.00 |
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