PELO (NM_015946) Human Tagged ORF Clone
CAT#: RC203592
PELO (Myc-DDK-tagged)-Human pelota homolog (Drosophila) (PELO)
"NM_015946" in other vectors (5)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | PELO |
Synonyms | CGI-17; PRO1770 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC203592 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCTCGTGAGGAAGAACATCGAGAAGGACAATGCGGGCCAGGTGACCCTGGTCCCCGAGGAGCCTG AGGACATGTGGCACACTTACAACCTCGTGCAGGTGGGCGACAGCCTGCGCGCCTCCACCATCCGCAAGGT ACAGACAGAGTCCTCCACGGGCAGCGTGGGCAGCAACCGGGTCCGCACTACCCTCACTCTCTGCGTGGAG GCCATCGACTTCGACTCTCAAGCCTGCCAGCTGCGGGTTAAGGGGACCAACATCCAAGAGAATGAGTATG TCAAGATGGGGGCTTACCACACCATCGAGCTGGAGCCCAACCGCCAGTTCACCCTGGCCAAGAAGCAGTG GGATAGTGTGGTACTGGAGCGCATCGAGCAGGCCTGTGACCCAGCCTGGAGCGCTGATGTGGCGGCTGTG GTCATGCAGGAAGGCCTCGCCCATATCTGCTTAGTCACTCCCAGCATGACCCTCACTCGGGCCAAGGTGG AGGTGAACATCCCTAGGAAAAGGAAAGGCAATTGCTCTCAGCATGACCGGGCCTTGGAGCGGTTCTATGA ACAGGTGGTCCAGGCTATCCAGCGCCACATACACTTTGATGTTGTAAAGTGCATCCTGGTGGCCAGCCCA GGATTTGTGAGGGAGCAGTTCTGCGACTACATGTTTCAACAAGCAGTGAAGACCGACAACAAACTGCTCC TGGAAAACCGGTCCAAATTTCTTCAGGTACATGCCTCCTCCGGACACAAGTACTCCCTGAAAGAGGCCCT TTGTGACCCTACTGTGGCTAGCCGCCTTTCAGACACTAAAGCTGCTGGGGAAGTCAAAGCCTTGGATGAC TTCTATAAAATGTTACAGCATGAACCGGATCGAGCTTTCTATGGACTCAAGCAGGTGGAGAAGGCCAATG AAGCCATGGCAATTGACACATTGCTCATCAGCGATGAGCTCTTCAGGCATCAGGATGTAGCCACACGGAG CCGGTATGTGAGGCTGGTGGACAGTGTGAAAGAGAATGCAGGCACCGTTAGGATATTCTCTAGTCTTCAC GTTTCTGGGGAACAGCTCAGCCAGTTGACTGGGGTAGCTGCCATTCTCCGCTTCCCTGTTCCCGAACTTT CTGACCAAGAGGGTGATTCCAGTTCTGAAGAGGAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203592 protein sequence
Red=Cloning site Green=Tags(s) MKLVRKNIEKDNAGQVTLVPEEPEDMWHTYNLVQVGDSLRASTIRKVQTESSTGSVGSNRVRTTLTLCVE AIDFDSQACQLRVKGTNIQENEYVKMGAYHTIELEPNRQFTLAKKQWDSVVLERIEQACDPAWSADVAAV VMQEGLAHICLVTPSMTLTRAKVEVNIPRKRKGNCSQHDRALERFYEQVVQAIQRHIHFDVVKCILVASP GFVREQFCDYMFQQAVKTDNKLLLENRSKFLQVHASSGHKYSLKEALCDPTVASRLSDTKAAGEVKALDD FYKMLQHEPDRAFYGLKQVEKANEAMAIDTLLISDELFRHQDVATRSRYVRLVDSVKENAGTVRIFSSLH VSGEQLSQLTGVAAILRFPVPELSDQEGDSSSEED TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_015946 |
ORF Size | 1155 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_015946.1, NM_015946.2, NM_015946.3, NM_015946.4, NP_057030.3 |
RefSeq Size | 2941 bp |
RefSeq ORF | 1158 bp |
Locus ID | 53918 |
Domains | eRF1_1, eRF1_2, eRF1_3 |
Protein Families | Druggable Genome |
MW | 43.4 kDa |
Gene Summary | This gene encodes a protein which contains a conserved nuclear localization signal. The encoded protein may have a role in spermatogenesis, cell cycle control, and in meiotic cell division. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC114538 | PELO (untagged)-Human pelota homolog (Drosophila) (PELO) |
USD 310.00 |
|
SC320778 | PELO (untagged)-Human pelota homolog (Drosophila) (PELO) |
USD 310.00 |
|
RG203592 | PELO (GFP-tagged) - Human pelota homolog (Drosophila) (PELO) |
USD 460.00 |
|
RC203592L3 | Lenti ORF clone of Human pelota homolog (Drosophila) (PELO), Myc-DDK-tagged |
USD 620.00 |
|
RC203592L4 | Lenti ORF clone of Human pelota homolog (Drosophila) (PELO), mGFP tagged |
USD 620.00 |
{0} Product Review(s)
Be the first one to submit a review