PELO (NM_015946) Human Tagged ORF Clone

CAT#: RG203592

PELO (GFP-tagged) - Human pelota homolog (Drosophila) (PELO)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PELO
• Synonyms: CGI-17; PRO1770
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG203592 representing NM_015946
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCTCGTGAGGAAGAACATCGAGAAGGACAATGCGGGCCAGGTGACCCTGGTCCCCGAGGAGCCTG
  AGGACATGTGGCACACTTACAACCTCGTGCAGGTGGGCGACAGCCTGCGCGCCTCCACCATCCGCAAGGT
  ACAGACAGAGTCCTCCACGGGCAGCGTGGGCAGCAACCGGGTCCGCACTACCCTCACTCTCTGCGTGGAG
  GCCATCGACTTCGACTCTCAAGCCTGCCAGCTGCGGGTTAAGGGGACCAACATCCAAGAGAATGAGTATG
  TCAAGATGGGGGCTTACCACACCATCGAGCTGGAGCCCAACCGCCAGTTCACCCTGGCCAAGAAGCAGTG
  GGATAGTGTGGTACTGGAGCGCATCGAGCAGGCCTGTGACCCAGCCTGGAGCGCTGATGTGGCGGCTGTG
  GTCATGCAGGAAGGCCTCGCCCATATCTGCTTAGTCACTCCCAGCATGACCCTCACTCGGGCCAAGGTGG
  AGGTGAACATCCCTAGGAAAAGGAAAGGCAATTGCTCTCAGCATGACCGGGCCTTGGAGCGGTTCTATGA
  ACAGGTGGTCCAGGCTATCCAGCGCCACATACACTTTGATGTTGTAAAGTGCATCCTGGTGGCCAGCCCA
  GGATTTGTGAGGGAGCAGTTCTGCGACTACATGTTTCAACAAGCAGTGAAGACCGACAACAAACTGCTCC
  TGGAAAACCGGTCCAAATTTCTTCAGGTACATGCCTCCTCCGGACACAAGTACTCCCTGAAAGAGGCCCT
  TTGTGACCCTACTGTGGCTAGCCGCCTTTCAGACACTAAAGCTGCTGGGGAAGTCAAAGCCTTGGATGAC
  TTCTATAAAATGTTACAGCATGAACCGGATCGAGCTTTCTATGGACTCAAGCAGGTGGAGAAGGCCAATG
  AAGCCATGGCAATTGACACATTGCTCATCAGCGATGAGCTCTTCAGGCATCAGGATGTAGCCACACGGAG
  CCGGTATGTGAGGCTGGTGGACAGTGTGAAAGAGAATGCAGGCACCGTTAGGATATTCTCTAGTCTTCAC
  GTTTCTGGGGAACAGCTCAGCCAGTTGACTGGGGTAGCTGCCATTCTCCGCTTCCCTGTTCCCGAACTTT
  CTGACCAAGAGGGTGATTCCAGTTCTGAAGAGGAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG203592 representing NM_015946
  Red=Cloning site Green=Tags(s)
  
  MKLVRKNIEKDNAGQVTLVPEEPEDMWHTYNLVQVGDSLRASTIRKVQTESSTGSVGSNRVRTTLTLCVE
  AIDFDSQACQLRVKGTNIQENEYVKMGAYHTIELEPNRQFTLAKKQWDSVVLERIEQACDPAWSADVAAV
  VMQEGLAHICLVTPSMTLTRAKVEVNIPRKRKGNCSQHDRALERFYEQVVQAIQRHIHFDVVKCILVASP
  GFVREQFCDYMFQQAVKTDNKLLLENRSKFLQVHASSGHKYSLKEALCDPTVASRLSDTKAAGEVKALDD
  FYKMLQHEPDRAFYGLKQVEKANEAMAIDTLLISDELFRHQDVATRSRYVRLVDSVKENAGTVRIFSSLH
  VSGEQLSQLTGVAAILRFPVPELSDQEGDSSSEED
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015946
• ORF Size: 1155 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_015946.4, NP_057030.3
• RefSeq Size: 2941
• RefSeq ORF: 1158
• Locus ID: 53918
• Domains: eRF1_1, eRF1_2, eRF1_3
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a protein which contains a conserved nuclear localization signal. The encoded protein may have a role in spermatogenesis, cell cycle control, and in meiotic cell division. [provided by RefSeq, Jul 2008]