DMAP1 (NM_001034023) Human Tagged ORF Clone

CAT#: RC203614

DMAP1 (Myc-DDK-tagged)-Human DNA methyltransferase 1 associated protein 1 (DMAP1), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY421951) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203614 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DMAP1
• Synonyms: DNMAP1; DNMTAP1; EAF2; MEAF2; SWC4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203614 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTACGGGCGCGGATGTACGGGACATTCTAGAACTCGGGGGTCCAGAAGGGGATGCAGCCTCTGGGA
  CCATCAGCAAGAAGGACATTATCAACCCGGACAAGAAAAAATCCAAGAAGTCCTCTGAGACACTGACTTT
  CAAGAGGCCCGAGGGCATGCACCGGGAAGTCTATGCCTTGCTCTACTCTGACAAGAAGGATGCACCCCCA
  CTGCTACCCAGTGACACTGGCCAGGGATACCGTACAGTGAAGGCCAAGTTGGGCTCCAAGAAGGTGCGGC
  CTTGGAAGTGGATGCCATTCACCAACCCGGCCCGCAAGGACGGAGCAATGTTCTTCCACTGGCGACGTGC
  AGCGGAGGAGGGCAAGGACTACCCCTTTGCCAGGTTCAATAAGACTGTGCAGGTGCCTGTGTACTCGGAG
  CAGGAGTACCAGCTTTATCTCCACGATGATGCTTGGACTAAGGCAGAAACTGACCACCTCTTTGACCTCA
  GCCGCCGCTTTGACCTGCGTTTTGTTGTTATCCATGACCGGTATGACCACCAGCAGTTCAAGAAGCGTTC
  TGTGGAAGACCTGAAGGAGCGGTACTACCACATCTGTGCTAAGCTTGCCAACGTGCGGGCTGTGCCAGGC
  ACAGACCTTAAGATACCAGTATTTGATGCTGGGCACGAACGACGGCGGAAGGAACAGCTTGAGCGTCTCT
  ACAACCGGACCCCAGAGCAGGTGGCAGAGGAGGAGTACCTGCTACAGGAGCTGCGCAAGATTGAGGCCCG
  GAAGAAGGAGCGGGAGAAACGCAGCCAGGACCTGCAGAAGCTGATCACAGCGGCAGACACCACTGCAGAG
  CAGCGGCGCACGGAACGCAAGGCCCCCAAAAAGAAGCTACCCCAGAAAAAGGAGGCTGAGAAGCCGGCTG
  TTCCTGAGACTGCAGGCATCAAGTTTCCAGACTTCAAGTCTGCAGGTGTCACGCTGCGGAGCCAACGGAT
  GAAGCTGCCAAGCTCTGTGGGACAGAAGAAGATCAAGGCCCTGGAACAGATGCTGCTGGAGCTTGGTGTG
  GAGCTGAGCCCGACACCTACGGAGGAGCTGGTGCACATGTTCAATGAGCTGCGAAGCGACCTGGTGCTGC
  TCTACGAGCTCAAGCAGGCCTGTGCCAACTGCGAGTATGAGCTGCAGATGCTGCGGCACCGTCATGAGGC
  ACTGGCCCGGGCTGGTGTGCTAGGGGGCCCTGCCACACCAGCATCAGGCCCAGGCCCGGCCTCTGCTGAG
  CCGGCAGTGACTGAACCCGGACTTGGTCCTGACCCCAAGGACACCATCATTGATGTGGTGGGCGCACCCC
  TCACGCCCAATTCGAGAAAGCGACGGGAGTCGGCCTCCAGCTCATCTTCCGTGAAGAAAGCCAAGAAGCC
  G
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203614 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MATGADVRDILELGGPEGDAASGTISKKDIINPDKKKSKKSSETLTFKRPEGMHREVYALLYSDKKDAPP
  LLPSDTGQGYRTVKAKLGSKKVRPWKWMPFTNPARKDGAMFFHWRRAAEEGKDYPFARFNKTVQVPVYSE
  QEYQLYLHDDAWTKAETDHLFDLSRRFDLRFVVIHDRYDHQQFKKRSVEDLKERYYHICAKLANVRAVPG
  TDLKIPVFDAGHERRRKEQLERLYNRTPEQVAEEEYLLQELRKIEARKKEREKRSQDLQKLITAADTTAE
  QRRTERKAPKKKLPQKKEAEKPAVPETAGIKFPDFKSAGVTLRSQRMKLPSSVGQKKIKALEQMLLELGV
  ELSPTPTEELVHMFNELRSDLVLLYELKQACANCEYELQMLRHRHEALARAGVLGGPATPASGPGPASAE
  PAVTEPGLGPDPKDTIIDVVGAPLTPNSRKRRESASSSSSVKKAKKP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001034023
• ORF Size: 1401 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001034023.1, NP_001029195.1
• RefSeq Size: 1603 bp
• RefSeq ORF: 1404 bp
• Locus ID: 55929
• Protein Families: Transcription Factors
• MW: 53 kDa
• Gene Summary: This gene encodes a subunit of several, distinct complexes involved in the repression or activation of transcription. The encoded protein can independently repress transcription and is targeted to replication foci throughout S phase by interacting directly with the N-terminus of DNA methyltransferase 1. During late S phase, histone deacetylase 2 is added to this complex, providing a means to deacetylate histones in transcriptionally inactive heterochromatin following replication. The encoded protein is also a component of the nucleosome acetyltransferase of H4 complex and interacts with the transcriptional corepressor tumor susceptibility gene 101 and the pro-apoptotic death-associated protein 6, among others. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008]