Treacher Collins syndrome protein (TCOF1) (NM_001008657) Human Tagged ORF Clone
CAT#: RC203644L4
- LentiORF®
Lenti ORF clone of Human Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant 3, mGFP tagged
"NM_001008657" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | mGFP |
Symbol | Treacher Collins syndrome protein |
Synonyms | MFD1; TCS; TCS1; treacle |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC203644).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_001008657 |
ORF Size | 2874 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001008657.1 |
RefSeq Size | 3877 bp |
RefSeq ORF | 2877 bp |
Locus ID | 6949 |
UniProt ID | Q13428 |
Cytogenetics | 5q32-q33.1 |
Protein Families | Druggable Genome, Stem cell - Pluripotency |
MW | 96.8 kDa |
Gene Summary | This gene encodes a nucleolar protein with a LIS1 homology domain. The protein is involved in ribosomal DNA gene transcription through its interaction with upstream binding factor (UBF). Mutations in this gene have been associated with Treacher Collins syndrome, a disorder which includes abnormal craniofacial development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC126432 | TCOF1 (untagged)-Human Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant 3 |
USD 760.00 |
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RC203644 | TCOF1 (Myc-DDK-tagged)-Human Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant 3 |
USD 750.00 |
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RG203644 | TCOF1 (GFP-tagged) - Human Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant 3 |
USD 830.00 |
|
RC203644L1 | Lenti ORF clone of Human Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant 3, Myc-DDK-tagged |
USD 1,176.00 |
|
RC203644L2 | Lenti ORF clone of Human Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant 3, mGFP tagged |
USD 950.00 |
|
RC203644L3 | Lenti ORF clone of Human Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant 3, Myc-DDK-tagged |
USD 950.00 |
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