Treacher Collins syndrome protein (TCOF1) (NM_001008657) Human Untagged Clone

CAT#: SC126432

TCOF1 (untagged)-Human Treacher Collins-Franceschetti syndrome 1 (TCOF1), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TCOF1
• Synonyms: MFD1; TCS; TCS1; treacle
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001008657 edited
  AGGTAGCCGGCCGGCCGGGGGTCGCGGGTATGGCCGAGGCCAGGAAGCGGCGGGAGCTAC
  TTCCCCTGATCTACCACCATCTGCTGCGGGCTGGCTATGTGCGTGCGGCGCGGGAAGTGA
  AGGAGCAGAGCGGCCAGAAGTGTTTCCTGGCTCAGCCCGTAACCCTTCTGGACATCTATA
  CACACTGGCAACAAACCTCAGAGCTTGGTCGGAAGCGGAAGGCAGAGGAAGATGCGGCAC
  TGCAAGCTAAGAAAACCCGTGTGTCAGACCCCATCAGCACCTCGGAGAGCTCGGAAGAGG
  AGGAAGAAGCAGAAGCCGAAACCGCCAAAGCCACCCCAAGACTAGCATCTACCAACTCCT
  CAGTCCTGGGGGCGGACTTGCCATCAAGCATGAAAGAAAAAGCCAAGGCAGAGACAGAGA
  AAGCTGGCAAGACTGGGAATTCCATGCCACACCCTGCCACTGGGAAGACGGTGGCCAACC
  TTCTTTCTGGGAAGTCTCCCAGGAAGTCAGCAGAGCCCTCAGCAAATACTACGTTGGTCT
  CAGAAACTGAGGAGGAGGGCAGCGTCCCGGCCTTTGGAGCTGCTGCCAAGCCTGGGATGG
  TGTCAGCGGGCCAGGCCGACAGCTCCAGCGAGGACACCTCCAGCTCCAGTGATGAGACAG
  ACGTGGAGGGGAAACCCTCAGTAAAACCAGCCCAGGTCAAAGCCTCATCAGTTTCTACTA
  AGGAGTCTCCAGCAAGAAAGGCGGCCCCAGCCCCTGGGAAGGTGGGGGATGTGACACCCC
  AGGTCAAAGGAGGGGCCCTGCCCCCAGCCAAGAGGGCCAAGAAGCCAGAAGAGGAGTCAG
  AGAGTAGTGAGGAGGGATCTGAAAGTGAGGAGGAGGCCCCTGCAGGGACACGAAGCCAGG
  TAAAGGCCTCTGAAAAAATTCTCCAGGTCAGAGCTGCCTCAGCCCCTGCCAAGGGGACCC
  CTGGGAAAGGGGCTACCCCAGCACCCCCTGGGAAGGCAGGGGCTGTAGCCTCCCAGACCA
  AGGCAGGGAAGCCAGAGGAGGACTCAGAGAGCAGCAGCGAGGAGTCATCTGACAGTGAGG
  AGGAGACGCCAGCTGCCAAGGCCCTGCTTCAGGCGAAGGCCTCAGGAAAAACCTCTCAGG
  TCGGAGCTGCCTCAGCCCCTGCCAAGGAGTCCCCCAGGAAAGGAGCTGCCCCAGCGCCCC
  CTGGGAAGACAGGGCCTGCAGTTGCCAAGGCCCAGGCGGGGAAGCGGGAGGAGGACTCGC
  AGAGCAGCAGCGAGGAATCGGACAGTGAGGAGGAGGCGCCTGCTCAGGCGAAGCCTTCAG
  GGAAGGCCCCCCAGGTCAGAGCCGCCTCGGCCCCTGCCAAGGAGTCCCCCAGGAAAGGGG
  CTGCCCCAGCACCTCCTAGGAAAACAGGGCCTGCAGCCGCCCAGGTCCAGGTGGGGAAGC
  AGGAGGAGGACTCAAGAAGCAGCAGCGAGGAGTCAGACAGTGACAGAGAGGCACTGGCAG
  CCATGAATGCAGCTCAGGTGAAGCCCTTGGGGAAAAGCCCCCAGGTGAAACCTGCCTCTA
  CCATGGGCATGGGGCCCTTGGGGAAAGGCGCCGGCCCAGTGCCACCCGGGAAGGTGGGGC
  CTGCAACCCCCTCAGCCCAGGTGGGGAAGTGGGAGGAGGACTCAGAGAGCAGTAGTGAGG
  AGTCATCAGACAGCAGTGATGGAGAGGTGCCCACAGCTGTGGCCCCGGCTCAGGAAAAGT
  CCTTGGGGAACATCCTCCAGGCCAAACCCACCTCCAGTCCTGCCAAGGGGCCCCCTCAGA
  AGGCAGGGCCTGTAGCCGTCCAGGTCAAGGCTGAAAAGCCCATGGACAACTCGGAGAGCA
  GCGAGGAGTCATCGGACAGTGCGGACAGTGAGGAGGCACCAGCAGCCATGACTGCAGCTC
  AGGCAAAACCAGCTCTGAAAATTCCTCAGACCAAGGCCTGCCCAAAGAAAACCAATACCA
  CTGCATCTGCCAAGGTCGCCCCTGTGCGAGTGGGCACCCAAGCCCCCCGGAAAGCAGGAA
  CTGCGACTTCTCCAGCAGGCTCATCCCCAGCTGTGGCTGGGGGCACCCAGAGACCAGCAG
  AGGATTCTTCAAGCAGTGAGGAATCAGATAGTGAGGAAGAGAAGACAGGTCTTGCAGTAA
  CCGTGGGACAGGCAAAGTCTGTGGGGAAAGGCCTCCAGGTGAAAGCAGCCTCAGTGCCTG
  TCAAGGGGTCCTTGGGGCAAGGGACTGCTCCAGTACTCCCTGGGAAGACGGGGCCTACAG
  TCACCCAGGTGAAAGCTGAAAAGCAGGAAGACTCTGAGAGCAGTGAGGAGGAATCAGACA
  GTGAGGAAGCAGCTGCATCTCCAGCACAGGTGAAAACCTCAGTAAAGAAAACCCAGGCCA
  AAGCCAACCCAGCTGCCGCCAGAGCACCTTCAGCAAAAGGGACAATTTCAGCCCCTGGAA
  AAGTTGTCACTGCAGCTGCTCAAGCCAAGCAGAGGTCTCCATCCAAGGTGAAGCCACCAG
  TGAGAAACCCCCAGAACAGTACCGTCTTGGCGAGGGGCCCAGCATCTGTGCCATCTGTGG
  GGAAGGCCGTGGCTACAGCAGCTCAGGCCCAGACAGGGCCAGAGGAGGACTCAGGGAGCA
  GTGAGGAGGAGTCAGACAGTGAGGAGGAGGCGGAGACGCTGGCTCAGGTGAAGCCTTCAG
  GGAAGACCCACCAGATCAGAGCTGCCTTGGCTCCTGCCAAGGAGTCCCCCAGGAAAGGGG
  CTGCCCCAACACCTCCTGGGAAGACAGGGCCTTCGGCTGCCCAGGCAGGGAAGCAGGATG
  ACTCAGGGAGCAGCAGCGAGGAATCAGACAGTGATGGGGAGGCACCGGCAGCTGTGACCT
  CTGCCCAGGACCAGGAGTCTTCTTGAATCCCATGAGCTCTGCCCAGGACCCTCTCTCAAG
  CACCTGTGGCATACCTTCATGTGCTCCACTGCACCCAGAGCTTAAGCCACAGTCCTGCTC
  AGGCTACACCTCCAAGGGTGGGACCAGGCCCACCTTATCTTCCTGTACTCCAGAGGCCCA
  AGTCAGCCATGCTGGTGTGGTCCAAGCTTCTGTGTGAACCCTGGCCCTTCCATCAGACAG
  CATTACCCTTTGTCCTCCTCTAGCCCCAAGCTCCCAGAAGCTTCTGCCAGGCACCTCAGA
  GCACCAGCTCCCCTGTCCCAGGGCCTAACCACAGATCCTGGAGTTCTGCAACCTCCAGAC
  CAGAGCGGTCTCATGAACCACCTCCCCTTGAATATCACATACCACATTGTTATCAATTCA
  TCAGTAAGGTCAGATGCTGTTTGCACACCTCCAGCAATAGGGTGCTCACTGCCTCTCACT
  TTCCAATGGCACAGATTACAAAAGTAAAGAACAACCACCAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001008657 unedited
  GGGTTAGATTTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGAGGTAGCC
  GGCCGGCCGGGGGTCGCGGGTATGGCCGAGGCCAGGAAGCGGCGGGAGCTACTTCCCCTG
  ATCTACCACCATCTGCTGCGGGCTGGCTATGTGCGTGCGGCGCGGGAAGTGAAGGAGCAG
  AGCGGCCAGAAGTGTTTCCTGGCTCAGCCCGTAACCCTTCTGGACATCTATACACACTGG
  CAACAAACCTCAGAGCTTGGTCGGAAGCGGAAGGCAGAGGAAGATGCGGCACTGCAAGCT
  AAGAAAACCCGTGTGTCAGACCCCATCAGCACCTCGGAGAGCTCGGAAGAGGAGGAAGAA
  GCAGAAGCCGAAACCGCCAAAGCCACCCCAAGACTAGCATCTACCAACTCCTCAGTCCTG
  GGGGCGGACTTGCCATCAAGCATGAAAGAAAAAGCCAAGGCAGAGACAGAGAAAGCTGGC
  AAGACTGGGAATTCCATGCCACACCCTGCCACTGGGAAGACGGTGGCCAACCTTCTTTCT
  GGGAAGTCTCCCAGGAAGTCAGCAGAGCCCTCAGCAAATACTACGTTGGTCTCAGAAACT
  GAGGAGGAGGGCAGCGTCCCGGCCTTTGGAGCTGCTGCCAAGCCTGGGATGGTGTCAGCG
  GGCCAGGCCGACAGCTCCAGCGAGGACACCTCCAGCTCCAGTGATGAGACAGACGTGGAG
  GGGAAACCCTCAGTAAAACCAGCCCCAGTCAAAGCCTCATCAGTTTCTACTAAGGAGTCT
  CCAGCAAGAAAGGCNGCCCCAGCCCCTGGGAGGTGGNGGGATGTGACACCCCCAGTCAAA
  GGAGGGGCCCTGCCCCCAGCCAAGAGGTCCAAGNAGCCNNNAGAGGAGTCAGAGAGTATT
  GAGGAGGGATCTGG
  
• Restriction Sites: Please inquire
• ACCN: NM_001008657
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001008657.1, NP_001008657.1
• RefSeq Size: 3827 bp
• RefSeq ORF: 2877 bp
• Locus ID: 6949
• Cytogenetics: 5q32-q33.1
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Gene Summary: 'This gene encodes a nucleolar protein with a LIS1 homology domain. The protein is involved in ribosomal DNA gene transcription through its interaction with upstream binding factor (UBF). Mutations in this gene have been associated with Treacher Collins syndrome, a disorder which includes abnormal craniofacial development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]'
  Transcript Variant: This variant (3) uses an alternate terminal exon and lacks 10 3' exons, compared to variant 4. The resulting isoform (c) has a significantly shorter and distinct C-terminus, compared to isoform d.