TFAP4 (NM_003223) Human Tagged ORF Clone

CAT#: RC203773

TFAP4 (Myc-DDK-tagged)-Human transcription factor AP-4 (activating enhancer binding protein 4) (TFAP4)

Product Images

Western blot validation of overexpression lysate (Cat# LY401113) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203773 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified TFAP4 protein (Cat# TP303773). The protein was produced from HEK293T cells transfected with TFAP4 cDNA clone (Cat# RC203773) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TFAP4
• Synonyms: AP-4; bHLHc41
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203773 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTATTTCATGGTGCCCACTCAGAAGGTGCCCTCTTTGCAACATTTCAGGAAAACAGAGAAAGAAG
  TGATAGGAGGGCTCTGTAGCCTTGCCAACATTCCACTAACCCCCGAGACTCAGCGGGACCAGGAGCGGCG
  GATTCGGCGGGAGATCGCCAACAGCAACGAGCGGAGACGCATGCAGAGCATCAACGCGGGATTCCAGTCC
  CTCAAGACCCTCATCCCCCACACAGACGGAGAGAAGCTCAGCAAGGCAGCCATTCTCCAGCAGACAGCCG
  AGTACATCTTCTCCCTGGAGCAGGAGAAGACCAGGCTCTTGCAGCAGAACACACAGCTCAAGCGCTTCAT
  CCAGGAGCTGAGCGGCTCGTCCCCCAAGCGACGGCGGGCAGAGGACAAGGACGAAGGCATAGGCTCCCCG
  GACATCTGGGAGGACGAGAAGGCGGAGGACCTGCGGCGGGAGATGATTGAGCTGCGGCAGCAGCTGGACA
  AGGAGCGCTCGGTGCGCATGATGCTGGAGGAGCAGGTGCGCTCGCTGGAGGCCCACATGTACCCGGAAAA
  GCTCAAGGTGATTGCGCAGCAGGTGCAGCTGCAGCAGCAGCAGGAACAGGTGAGGCTGCTGCACCAGGAG
  AAGCTGGAGCGGGAACAGCAGCAGCTGCGGACCCAGCTTCTGCCCCCTCCGGCCCCCACCCACCACCCCA
  CGGTGATCGTGCCAGCACCGCCTCCTCCTCCCTCCCACCACATCAATGTCGTCACCATGGGCCCCTCCTC
  GGTCATCAACTCTGTTTCCACATCCCGGCAAAATCTGGACACCATCGTGCAGGCAATCCAGCACATCGAG
  GGCACCCAGGAAAAGCAGGAGCTGGAGGAGGAGCAGCGGCGAGCTGTCATCGTGAAGCCTGTCCGCAGCT
  GCCCGGAGGCCCCCACCTCTGACACCGCCTCCGACTCCGAGGCCTCAGACAGTGACGCCATGGACCAGAG
  CCGGGAGGAGCCGTCGGGGGACGGGGAGCTTCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203773 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEYFMVPTQKVPSLQHFRKTEKEVIGGLCSLANIPLTPETQRDQERRIRREIANSNERRRMQSINAGFQS
  LKTLIPHTDGEKLSKAAILQQTAEYIFSLEQEKTRLLQQNTQLKRFIQELSGSSPKRRRAEDKDEGIGSP
  DIWEDEKAEDLRREMIELRQQLDKERSVRMMLEEQVRSLEAHMYPEKLKVIAQQVQLQQQQEQVRLLHQE
  KLEREQQQLRTQLLPPPAPTHHPTVIVPAPPPPPSHHINVVTMGPSSVINSVSTSRQNLDTIVQAIQHIE
  GTQEKQELEEEQRRAVIVKPVRSCPEAPTSDTASDSEASDSDAMDQSREEPSGDGELP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003223
• ORF Size: 1014 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003223.1, NM_003223.2, NP_003214.1
• RefSeq Size: 2147 bp
• RefSeq ORF: 1017 bp
• Locus ID: 7023
• Cytogenetics: 16p13.3
• Protein Families: Druggable Genome, Transcription Factors
• MW: 38.7 kDa
• Gene Summary: 'Transcription factors of the basic helix-loop-helix-zipper (bHLH-ZIP) family contain a basic domain, which is used for DNA binding, and HLH and ZIP domains, which are used for oligomerization. Transcription factor AP4 activates both viral and cellular genes by binding to the symmetrical DNA sequence CAGCTG (Mermod et al., 1988 [PubMed 2833704]; Hu et al., 1990 [PubMed 2123466]).[supplied by OMIM, Jul 2009]'