SMNDC1 (NM_005871) Human Tagged ORF Clone

CAT#: RC203791

SMNDC1 (Myc-DDK-tagged)-Human survival motor neuron domain containing 1 (SMNDC1)

Product Images

Western blot validation of overexpression lysate (Cat# LY417013) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203791 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified SMNDC1 protein (Cat# TP303791). The protein was produced from HEK293T cells transfected with SMNDC1 cDNA clone (Cat# RC203791) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SMNDC1
• Synonyms: SMNR; SPF30; TDRD16C
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203791 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGAGGATTTAGCAAAGCAGCTGGCAAGCTACAAAGCTCAGCTCCAGCAAGTTGAAGCTGCATTAT
  CTGGAAATGGAGAAAATGAAGATTTGCTAAAATTGAAGAAAGATTTACAAGAAGTTATAGAACTAACCAA
  AGACCTTCTGTCAACTCAACCTTCTGAGACGCTTGCAAGTTCAGACAGTTTTGCTTCTACTCAACCTACT
  CATTCATGGAAAGTAGGAGACAAGTGTATGGCAGTCTGGAGTGAAGATGGACAGTGTTATGAAGCGGAGA
  TTGAGGAGATAGATGAAGAAAATGGCACCGCTGCAATCACCTTTGCTGGTTATGGCAATGCTGAAGTGAC
  TCCACTGTTGAACCTCAAGCCTGTAGAAGAAGGAAGGAAGGCAAAGGAGGACAGTGGCAACAAACCCATG
  TCAAAAAAAGAAATGATTGCCCAGCAGCGTGAATATAAAAAGAAGAAAGCTTTGAAAAAAGCTCAGAGAA
  TAAAAGAACTTGAGCAGGAAAGAGAGGACCAGAAAGTGAAATGGCAACAATTCAACAACAGAGCCTATTC
  TAAAAACAAAAAAGGCCAGGTAAAGAGGAGTATTTTTGCTTCACCTGAGAGTGTGACTGGTAAAGTTGGA
  GTAGGAACCTGTGGAATTGCTGATAAACCTATGACACAATATCAAGATACCTCTAAATACAATGTCAGGC
  ATTTGATGCCTCAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203791 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSEDLAKQLASYKAQLQQVEAALSGNGENEDLLKLKKDLQEVIELTKDLLSTQPSETLASSDSFASTQPT
  HSWKVGDKCMAVWSEDGQCYEAEIEEIDEENGTAAITFAGYGNAEVTPLLNLKPVEEGRKAKEDSGNKPM
  SKKEMIAQQREYKKKKALKKAQRIKELEQEREDQKVKWQQFNNRAYSKNKKGQVKRSIFASPESVTGKVG
  VGTCGIADKPMTQYQDTSKYNVRHLMPQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005871
• ORF Size: 714 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005871.1, NM_005871.2, NM_005871.3, NP_005862.1
• RefSeq Size: 2043 bp
• RefSeq ORF: 717 bp
• Locus ID: 10285
• Domains: TUDOR
• Protein Families: Stem cell - Pluripotency
• Protein Pathways: Spliceosome
• MW: 26.7 kDa
• Gene Summary: This gene is a paralog of SMN1 gene, which encodes the survival motor neuron protein, mutations in which are cause of autosomal recessive proximal spinal muscular atrophy. The protein encoded by this gene is a nuclear protein that has been identified as a constituent of the spliceosome complex. This gene is differentially expressed, with abundant levels in skeletal muscle, and may share similar cellular function as the SMN1 gene. [provided by RefSeq, Jul 2008]