Sphingomyelin Synthase 1 (SGMS1) (NM_147156) Human Tagged ORF Clone

CAT#: RC203853

SGMS1 (Myc-DDK-tagged)-Human sphingomyelin synthase 1 (SGMS1)

Product Images

Western blot validation of overexpression lysate (Cat# LY407819) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203853 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SGMS1
• Synonyms: hmob33; MOB; MOB1; SMS1; TMEM23
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203853 representing NM_147156
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGAAGTGGTTTATTGGTCACCCAAGAAGGTGGCAGACTGGCTGCTGGAGAATGCTATGCCAGAAT
  ACTGTGAGCCTCTGGAGCATTTCACAGGCCAGGACTTGATCAACCTAACCCAAGAGGATTTCAAAAAACC
  CCCCTTGTGCCGAGTCTCCTCTGACAACGGGCAGCGGCTCCTGGACATGATAGAAACCCTGAAAATGGAG
  CACCATTTGGAAGCACACAAGAACGGCCATGCCAATGGGCACCTCAACATTGGCGTAGACATCCCCACCC
  CCGACGGCAGCTTCAGCATCAAGATTAAACCCAACGGGATGCCAAATGGGTATAGGAAAGAGATGATAAA
  GATCCCCATGCCAGAACTGGAGCGCTCTCAGTACCCCATGGAGTGGGGCAAGACTTTTCTGGCCTTTCTT
  TATGCACTTTCCTGTTTCGTTCTCACCACAGTGATGATCTCGGTCGTCCACGAACGAGTACCTCCTAAGG
  AGGTGCAGCCTCCACTACCGGACACATTTTTTGACCATTTTAACCGGGTGCAGTGGGCCTTTTCTATTTG
  TGAAATTAATGGCATGATCCTTGTAGGACTCTGGTTAATTCAGTGGCTGCTCTTAAAATACAAGTCTATT
  ATTAGCAGAAGATTTTTCTGCATAGTTGGCACGCTGTACCTGTATCGGTGTATTACAATGTATGTAACTA
  CACTCCCAGTACCTGGTATGCATTTCAACTGTTCTCCGAAGCTTTTCGGAGACTGGGAAGCCCAACTGCG
  AAGAATAATGAAGCTCATTGCTGGAGGTGGCTTGTCTATCACTGGCTCTCACAACATGTGTGGGGACTAT
  CTGTACAGCGGCCACACGGTCATGCTAACACTTACCTACTTATTTATCAAAGAGTATTCCCCTCGGCGAC
  TCTGGTGGTATCACTGGATTTGCTGGCTTCTCAGCGTAGTTGGAATCTTCTGTATTCTCTTAGCGCATGA
  CCACTACACTGTGGACGTGGTGGTGGCATATTACATCACCACGAGACTCTTCTGGTGGTATCACACTATG
  GCCAATCAGCAAGTGCTAAAGGAAGCTTCCCAGATGAACCTCCTGGCCAGGGTGTGGTGGTACAGGCCAT
  TTCAGTACTTTGAAAAGAATGTCCAAGGAATTGTACCTCGATCTTACCATTGGCCTTTCCCCTGGCCAGT
  AGTCCACCTCAGTAGGCAAGTTAAATACAGCCGGCTGGTGAATGACACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203853 representing NM_147156
  Red=Cloning site Green=Tags(s)
  
  MKEVVYWSPKKVADWLLENAMPEYCEPLEHFTGQDLINLTQEDFKKPPLCRVSSDNGQRLLDMIETLKME
  HHLEAHKNGHANGHLNIGVDIPTPDGSFSIKIKPNGMPNGYRKEMIKIPMPELERSQYPMEWGKTFLAFL
  YALSCFVLTTVMISVVHERVPPKEVQPPLPDTFFDHFNRVQWAFSICEINGMILVGLWLIQWLLLKYKSI
  ISRRFFCIVGTLYLYRCITMYVTTLPVPGMHFNCSPKLFGDWEAQLRRIMKLIAGGGLSITGSHNMCGDY
  LYSGHTVMLTLTYLFIKEYSPRRLWWYHWICWLLSVVGIFCILLAHDHYTVDVVVAYYITTRLFWWYHTM
  ANQQVLKEASQMNLLARVWWYRPFQYFEKNVQGIVPRSYHWPFPWPVVHLSRQVKYSRLVNDT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_147156
• ORF Size: 1239 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_147156.1, NM_147156.2, NM_147156.3, NP_671512.1
• RefSeq Size: 3767
• RefSeq ORF: 1242
• Locus ID: 259230
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, Sphingolipid metabolism
• MW: 48.4 kDa
• Gene Summary: The protein encoded by this gene is predicted to be a five-pass transmembrane protein. This gene may be predominately expressed in brain. [provided by RefSeq, Jul 2008]