KMT5C (NM_032701) Human Tagged ORF Clone

CAT#: RC203881

SUV420H2 (Myc-DDK-tagged)-Human suppressor of variegation 4-20 homolog 2 (Drosophila) (SUV420H2)

Product Images

Western blot validation of overexpression lysate (Cat# LY409973) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203881 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KMT5C
• Synonyms: Suv4-20h2; SUV420H2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203881 representing NM_032701
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCCCGACAGAGTGACAGCACGAGAACTGTGCGAGAACGACGACCTGGCCACCAGCCTCGTCCTGG
  ACCCCTACCTCGGTTTCCGCACCCATAAGATGAACGTCAGCCCTGTGCCCCCCCTGCGGCGACAGCAGCA
  CCTGCGCTCAGCGCTGGAAACTTTCCTGAGGCAGCGGGACCTGGAGGCTGCGTACCGGGCCCTGACGCTG
  GGAGGCTGGACGGCCCGCTACTTCCAGAGCCGGGGCCCGCGGCAGGAGGCTGCCCTCAAGACCCACGTCT
  ATCGCTACCTCCGTGCCTTCCTGCCGGAAAGTGGCTTTACCATCCTGCCCTGCACGCGCTACTCCATGGA
  GACCAACGGGGCCAAGATCGTGTCCACTCGTGCTTGGAAAAAGAATGAGAAGCTGGAGCTGCTGGTGGGC
  TGCATTGCAGAGCTGCGGGAGGCAGATGAGGGGCTGCTGAGGGCCGGTGAGAATGACTTCAGCATCATGT
  ACTCAACCCGCAAGCGGAGTGCTCAGCTGTGGCTGGGCCCAGCCGCCTTCATCAACCATGACTGCAAACC
  CAACTGCAAGTTTGTGCCTGCAGATGGGAACGCAGCCTGCGTGAAGGTGCTCCGGGACATTGAGCCTGGG
  GACGAGGTGACATGCTTCTACGGCGAGGGCTTCTTCGGCGAGAAGAATGAGCACTGTGAATGCCACACCT
  GTGAGAGGAAAGGTGAAGGAGCTTTCCGAACCAGGCCTAGGGAGCCCGCGTTGCCACCACGGCCCCTGGA
  CAAGTACCAGCTGCGTGAGACCAAGCGGCGGCTGCAGCAAGGCCTGGACAGTGGCAGCCGACAGGGCCTG
  CTGGGCCCTCGGGCCTGCGTGCACCCATCCCCGCTGCGCCGGGACCCATTCTGCGCCGCCTGCCAGCCCC
  TGCGCCTGCCAGCCTGCAGCGCCCGCCCAGACACCTCACCCCTCTGGCTCCAGTGGCTGCCTCAGCCCCA
  GCCCCGAGTGCGGCCCCGGAAGCGCCGACGCCCCCGGCCCCGGAGGGCCCCAGTGCTCTCCACCCACCAC
  GCTGCCCGCGTCTCCCTGCACCGATGGGGAGGCTGTGGCCCCCACTGCCGCCTGCGAGGAGAGGCCCTGG
  TGGCCCTGGGCCAGCCCCCCCACGCCCGCTGGGCCCCTCAGCAGGACTGGCACTGGGCCCGGCGCTATGG
  GCTGCCTTACGTGGTGCGTGTGGACCTTCGTCGCCTGGCCCCAGCCCCACCAGCTACCCCAGCCCCTGCT
  GGGACCCCAGGCCCCATCCTGATCCCGAAGCAGGCCCTCGCCTTCGCCCCCTTCTCCCCACCCAAGCGCC
  TACGGCTGGTGGTCAGCCACGGCTCCATCGACCTGGATGTCGGCGGTGAAGAGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203881 representing NM_032701
  Red=Cloning site Green=Tags(s)
  
  MGPDRVTARELCENDDLATSLVLDPYLGFRTHKMNVSPVPPLRRQQHLRSALETFLRQRDLEAAYRALTL
  GGWTARYFQSRGPRQEAALKTHVYRYLRAFLPESGFTILPCTRYSMETNGAKIVSTRAWKKNEKLELLVG
  CIAELREADEGLLRAGENDFSIMYSTRKRSAQLWLGPAAFINHDCKPNCKFVPADGNAACVKVLRDIEPG
  DEVTCFYGEGFFGEKNEHCECHTCERKGEGAFRTRPREPALPPRPLDKYQLRETKRRLQQGLDSGSRQGL
  LGPRACVHPSPLRRDPFCAACQPLRLPACSARPDTSPLWLQWLPQPQPRVRPRKRRRPRPRRAPVLSTHH
  AARVSLHRWGGCGPHCRLRGEALVALGQPPHARWAPQQDWHWARRYGLPYVVRVDLRRLAPAPPATPAPA
  GTPGPILIPKQALAFAPFSPPKRLRLVVSHGSIDLDVGGEEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032701
• ORF Size: 1386 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032701.2, NM_032701.3, NP_116090.2
• RefSeq Size: 2297 bp
• RefSeq ORF: 1389 bp
• Locus ID: 84787
• Protein Families: Druggable Genome
• Protein Pathways: Lysine degradation
• MW: 51.9 kDa
• Gene Summary: SUV420H2 and the related enzyme SUV420H1 (MIM 610881) function as histone methyltransferases that specifically trimethylate nucleosomal histone H4 (see MIM 602822) on lysine-20 (K20) (Schotta et al., 2004 [PubMed 15145825]). [supplied by OMIM, Dec 2009]