BEN Domain Containing Protein 5 (BEND5) (NM_024603) Human Tagged ORF Clone

CAT#: RC203968

BEND5 (Myc-DDK-tagged)-Human BEN domain containing 5 (BEND5)

Product Images

Coomassie blue staining of purified BEND5 protein (Cat# TP303968). The protein was produced from HEK293T cells transfected with BEND5 cDNA clone (Cat# RC203968) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BEND5
• Synonyms: C1orf165
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203968 representing NM_024603
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACGCCTTTGTGCGGTTCCTGGAGGACAACGTCTGCTACGCGCTGCCCGTGTCGTGCGTGCGCGACT
  TCAGCCCCCGCTCGCGGCTGGATTTTGACAACCAGAAGGTGTACGCCGTGTACCGGGGCCCGGAGGAATT
  GGGCGCCGGGCCCGAGAGCCCCCCGCGCGCCCCCCGCGACTGGGGCGCGCTGTTGCTCCACAAGGCCCAG
  ATCCTGGCGCTGGCAGAAGACAAATCTGACCTTGAAAACAGTGTGATGCAGAAGAAAATAAAAATCCCCA
  AGCTTTCTCTTAATCATGTAGAAGAAGATGGAGAGGTTAAAGATTATGGGGAAGAAGATTTACAGCTTAG
  ACACATCAAGAGACCTGAGGGGCGGAAGCCGAGCGAAGTGGCGCACAAGAGCATCGAGGCAGTGGTGGCT
  CGGCTAGAGAAGCAGAACGGCCTGAGCCTGGGCCATAGCACGTGTCCGGAAGAGGTCTTCGTGGAGGCCT
  CGCCAGGCACAGAGGACATGGACAGTCTAGAAGATGCTGTGGTGCCCCGGGCTCTGTATGAGGAGCTGCT
  GCGCAACTACCAGCAGCAACAGGAAGAGATGCGCCACCTCCAGCAGGAGCTGGAGCGGACTCGGAGGCAG
  CTGGTACAACAGGCCAAGAAGCTCAAGGAGTACGGGGCACTTGTGTCTGAAATGAAGGAGCTCCGTGACC
  TTAACCGGAGGCTCCAGGACGTGCTGCTCCTGCGGCTTGGCAGCGGTCCCGCCATTGATCTGGAAAAAGT
  AAAGTCAGAATGTCTCGAGCCCGAGCCGGAGTTACGGAGCACTTTCAGTGAGGAAGCAAATACGTCGTCC
  TATTACCCCGCTCCTGCGCCTGTCATGGACAAGTATATCCTAGACAATGGCAAGGTCCATCTGGGAAGCG
  GGATTTGGGTTGATGAGGAGAAATGGCACCAGCTACAAGTAACCCAAGGAGATTCCAAGTACACGAAGAA
  CTTGGCAGTTATGATTTGGGGAACAGATGTTCTGAAAAACAGAAGCGTCACAGGCGTCGCCACAAAAAAA
  AAGAAAGATGCAGTCCCTAAACCACCCCTCTCGCCTCACAAACTAAGCATCGTCAGAGAGTGTTTGTATG
  ACAGAATAGCACAAGAAACTGTGGATGAAACTGAAATTGCACAGAGACTCTCCAAAGTCAACAAGTACAT
  CTGTGAAAAAATCATGGATATCAATAAATCCTGTAAAAATGAAGAACGAAGGGAAGCAAAATACAATTTG
  CAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203968 representing NM_024603
  Red=Cloning site Green=Tags(s)
  
  MYAFVRFLEDNVCYALPVSCVRDFSPRSRLDFDNQKVYAVYRGPEELGAGPESPPRAPRDWGALLLHKAQ
  ILALAEDKSDLENSVMQKKIKIPKLSLNHVEEDGEVKDYGEEDLQLRHIKRPEGRKPSEVAHKSIEAVVA
  RLEKQNGLSLGHSTCPEEVFVEASPGTEDMDSLEDAVVPRALYEELLRNYQQQQEEMRHLQQELERTRRQ
  LVQQAKKLKEYGALVSEMKELRDLNRRLQDVLLLRLGSGPAIDLEKVKSECLEPEPELRSTFSEEANTSS
  YYPAPAPVMDKYILDNGKVHLGSGIWVDEEKWHQLQVTQGDSKYTKNLAVMIWGTDVLKNRSVTGVATKK
  KKDAVPKPPLSPHKLSIVRECLYDRIAQETVDETEIAQRLSKVNKYICEKIMDINKSCKNEERREAKYNL
  Q
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024603
• ORF Size: 1263 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024603.2, NM_024603.3, NP_078879.2
• RefSeq Size: 1781
• RefSeq ORF: 1266
• Locus ID: 79656
• MW: 48.2 kDa