DNAJC7 (NM_003315) Human Tagged ORF Clone

CAT#: RC204154

DNAJC7 (Myc-DDK-tagged)-Human DnaJ (Hsp40) homolog, subfamily C, member 7 (DNAJC7), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY418777) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204154 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified DNAJC7 protein (Cat# TP304154). The protein was produced from HEK293T cells transfected with DNAJC7 cDNA clone (Cat# RC204154) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DNAJC7
• Synonyms: DJ11; DJC7; TPR2; TTC2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204154 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCTGCCGCGGAGTGCGATGTGGTAATGGCGGCGACCGAGCCGGAGCTGCTCGACGACCAAGAGG
  CGAAGAGGGAAGCAGAGACTTTCAAGGAACAAGGAAATGCATACTATGCCAAGAAAGATTACAATGAAGC
  TTATAATTATTATACAAAAGCCATAGATATGTGTCCTAAAAATGCTAGCTATTATGGTAATCGAGCAGCC
  ACCTTGATGATGCTTGGAAGGTTCCGGGAAGCTCTTGGAGATGCACAACAGTCAGTGAGGTTGGATGACA
  GTTTTGTCCGGGGACATCTACGAGAGGGCAAGTGCCACCTCTCTCTGGGGAATGCCATGGCAGCATGTCG
  CAGCTTCCAGAGAGCCCTAGAACTGGATCATAAAAATGCTCAGGCACAACAAGAGTTCAAGAATGCTAAT
  GCAGTCATGGAATATGAGAAAATAGCAGAAACAGATTTTGAGAAGCGAGATTTTCGGAAGGTTGTTTTCT
  GCATGGACCGTGCCCTAGAATTTGCCCCTGCCTGCCATCGCTTCAAAATCCTCAAGGCAGAATGTTTAGC
  AATGCTGGGTCGTTATCCAGAAGCACAGTCTGTGGCTAGTGACATTCTACGAATGGATTCCACCAATGCA
  GATGCTCTGTATGTACGAGGTCTTTGCCTTTATTACGAAGATTGTATTGAGAAGGCAGTTCAGTTTTTCG
  TACAGGCTCTCAGGATGGCTCCTGACCACGAGAAGGCCTGCATTGCCTGCAGAAATGCCAAAGCACTCAA
  AGCAAAGAAAGAAGATGGGAATAAAGCATTTAAGGAAGGAAATTACAAACTAGCATATGAACTGTACACA
  GAAGCCCTGGGGATAGACCCCAACAATATAAAAACAAATGCTAAACTCTACTGTAATCGGGGTACGGTTA
  ATTCCAAGCTTAGGAAACTAGATGATGCAATAGAAGACTGCACAAATGCAGTGAAGCTTGATGACACTTA
  CATAAAAGCCTACTTGAGAAGAGCTCAGTGTTACATGGACACAGAACAGTATGAAGAAGCAGTACGAGAC
  TATGAAAAAGTATACCAGACAGAGAAAACAAAAGAACACAAACAGCTCCTAAAAAATGCGCAGCTGGAAC
  TGAAGAAGAGTAAGAGGAAAGATTACTACAAGATTCTAGGAGTGGACAAGAATGCCTCTGAGGACGAGAT
  CAAGAAAGCTTATCGGAAACGGGCCTTGATGCACCATCCAGATCGGCATAGTGGAGCCAGTGCTGAGGTT
  CAGAAGGAGGAGGAGAAGAAGTTCAAGGAAGTTGGAGAGGCCTTTACTATCCTCTCTGATCCCAAGAAAA
  AGACTCGCTATGACAGTGGACAGGACCTAGATGAGGAGGGCATGAATATGGGTGATTTTGATCCAAACAA
  TATCTTCAAGGCATTCTTTGGCGGTCCTGGCGGCTTCAGCTTTGAAGCATCTGGTCCAGGGAATTTCTTT
  TTTCAATTTGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204154 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAAAECDVVMAATEPELLDDQEAKREAETFKEQGNAYYAKKDYNEAYNYYTKAIDMCPKNASYYGNRAA
  TLMMLGRFREALGDAQQSVRLDDSFVRGHLREGKCHLSLGNAMAACRSFQRALELDHKNAQAQQEFKNAN
  AVMEYEKIAETDFEKRDFRKVVFCMDRALEFAPACHRFKILKAECLAMLGRYPEAQSVASDILRMDSTNA
  DALYVRGLCLYYEDCIEKAVQFFVQALRMAPDHEKACIACRNAKALKAKKEDGNKAFKEGNYKLAYELYT
  EALGIDPNNIKTNAKLYCNRGTVNSKLRKLDDAIEDCTNAVKLDDTYIKAYLRRAQCYMDTEQYEEAVRD
  YEKVYQTEKTKEHKQLLKNAQLELKKSKRKDYYKILGVDKNASEDEIKKAYRKRALMHHPDRHSGASAEV
  QKEEEKKFKEVGEAFTILSDPKKKTRYDSGQDLDEEGMNMGDFDPNNIFKAFFGGPGGFSFEASGPGNFF
  FQFG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003315
• ORF Size: 1485 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003315.2, NM_003315.3, NP_003306.3
• RefSeq Size: 2096 bp
• RefSeq ORF: 1485 bp
• Locus ID: 7266
• Cytogenetics: 17q21.2
• Domains: TPR, DnaJ
• MW: 56.4 kDa
• Gene Summary: 'This gene encodes a member of the DNAJ heat shock protein 40 family of proteins that is characterized by two N-terminal tetratricopeptide repeat domains and a C-terminal DNAJ domain. This protein binds the chaperone proteins heat shock proteins 70 and 90 in an ATP-dependent manner and may function as a co-chaperone. Pseudogenes of this gene are found on chromosomes 1 and 6. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2009]'