BIGM103 (SLC39A8) (NM_022154) Human Tagged ORF Clone

CAT#: RC204200

SLC39A8 (Myc-DDK-tagged)-Human solute carrier family 39 (zinc transporter), member 8 (SLC39A8), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY427559) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC225773 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SLC39A8
• Synonyms: BIGM103; CDG2N; LZT-Hs6; PP3105; ZIP8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204200 representing NM_022154
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCCGGGTCGCGCGGTGGCCGGGCTCCTGTTGCTGGCGGCCGCCGGCCTCGGAGGAGTGGCGGAGG
  GGCCAGGGCTAGCCTTCAGCGAGGATGTGCTGAGCGTGTTCGGCGCGAATCTGAGCCTGTCGGCGGCGCA
  GCTCCAGCACTTGCTGGAGCAGATGGGAGCCGCCTCCCGCGTGGGCGTCCCGGAGCCTGGCCAGCTGCAC
  TTCAACCAGTGTTTAACTGCTGAAGAGATCTTTTCCCTTCATGGCTTTTCAAATGCTACCCAAATAACCA
  GCTCCAAATTCTCTGTCATCTGTCCAGCAGTCTTACAGCAATTGAACTTTCACCCATGTGAGGATCGGCC
  CAAGCACAAAACAAGACCAAGTCATTCAGAAGTTTGGGGATATGGATTCCTGTCAGTGACGATTATTAAT
  CTGGCATCTCTCCTCGGATTGATTTTGACTCCACTGATAAAGAAATCTTATTTCCCAAAGATTTTGACCT
  TTTTTGTGGGGCTGGCTATTGGGACTCTTTTTTCAAATGCAATTTTCCAACTTATTCCAGAGGCATTTGG
  ATTTGATCCCAAAGTCGACAGTTATGTTGAGAAGGCAGTTGCTGTGTTTGGTGGATTTTACCTACTTTTC
  TTTTTTGAAAGAATGCTAAAGATGTTATTAAAGACATATGGTCAGAATGGTCATACCCACTTTGGAAATG
  ATAACTTTGGTCCTCAAGAAAAAACTCATCAACCTAAAGCATTACCTGCCATCAATGGTGTGACATGCTA
  TGCAAATCCTGCTGTCACAGAAGCTAATGGACATATCCATTTTGATAATGTCAGTGTGGTATCTCTACAG
  GATGGAAAAAAAGAGCCAAGTTCATGTACCTGTTTGAAGGGGCCCAAACTGTCAGAAATAGGGACGATTG
  CCTGGATGATAACGCTCTGCGATGCCCTCCACAATTTCATCGATGGCCTGGCGATTGGGGCTTCCTGCAC
  CTTGTCTCTCCTTCAGGGACTCAGTACTTCCATAGCAATCCTATGTGAGGAGTTTCCCCACGAGTTAGGA
  GACTTTGTGATCCTACTCAATGCAGGGATGAGCACTCGACAAGCCTTGCTATTCAACTTCCTTTCTGCAT
  GTTCCTGCTATGTTGGGCTAGCTTTTGGCATTTTGGTGGGCAACAATTTCGCTCCAAATATTATATTTGC
  ACTTGCTGGAGGCATGTTCCTCTATATTTCTCTGGCAGATATGTTTCCAGAGATGAATGATATGCTGAGA
  GAAAAGGTAACTGGAAGAAAAACCGATTTCACCTTCTTCATGATTCAGAATGCTGGAATGTTAACTGGAT
  TCACAGCCATTCTACTCATTACCTTGTATGCAGGAGAAATCGAATTGGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204200 representing NM_022154
  Red=Cloning site Green=Tags(s)
  
  MAPGRAVAGLLLLAAAGLGGVAEGPGLAFSEDVLSVFGANLSLSAAQLQHLLEQMGAASRVGVPEPGQLH
  FNQCLTAEEIFSLHGFSNATQITSSKFSVICPAVLQQLNFHPCEDRPKHKTRPSHSEVWGYGFLSVTIIN
  LASLLGLILTPLIKKSYFPKILTFFVGLAIGTLFSNAIFQLIPEAFGFDPKVDSYVEKAVAVFGGFYLLF
  FFERMLKMLLKTYGQNGHTHFGNDNFGPQEKTHQPKALPAINGVTCYANPAVTEANGHIHFDNVSVVSLQ
  DGKKEPSSCTCLKGPKLSEIGTIAWMITLCDALHNFIDGLAIGASCTLSLLQGLSTSIAILCEEFPHELG
  DFVILLNAGMSTRQALLFNFLSACSCYVGLAFGILVGNNFAPNIIFALAGGMFLYISLADMFPEMNDMLR
  EKVTGRKTDFTFFMIQNAGMLTGFTAILLITLYAGEIELE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022154
• ORF Size: 1380 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022154.1, NM_022154.2, NM_022154.4, NM_022154.5, NP_071437.3
• RefSeq Size: 3309 bp
• RefSeq ORF: 1383 bp
• Locus ID: 64116
• Domains: Zip
• Protein Families: Transmembrane
• MW: 49.5 kDa
• Gene Summary: This gene encodes a member of the SLC39 family of solute-carrier genes, which show structural characteristics of zinc transporters. The encoded protein is glycosylated and found in the plasma membrane and mitochondria, and functions in the cellular import of zinc at the onset of inflammation. It is also thought to be the primary transporter of the toxic cation cadmium, which is found in cigarette smoke. Multiple transcript variants encoding different isoforms have been found for this gene. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Oct 2008]