CRLF3 (NM_015986) Human Tagged ORF Clone

CAT#: RC204326

CRLF3 (Myc-DDK-tagged)-Human cytokine receptor-like factor 3 (CRLF3)

Product Images

Western blot validation of overexpression lysate (Cat# LY414260) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204326 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified CRLF3 protein (Cat# TP304326). The protein was produced from HEK293T cells transfected with CRLF3 cDNA clone (Cat# RC204326) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CRLF3
• Synonyms: CREME-9; CREME9; CRLM9; CYTOR4; FRWS; p48.2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204326 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGGGGGCGATGGAGCTGGAGCCTGAGCTGCTGTTGCAGGAGGCCCGCGAGAACGTGGAGGCAGCGC
  AGAGCTACCGGCGGGAGCTGGGTCACCGGCTTGAGGGGCTGCGTGAGGCGCGGAGGCAGATCAAAGAAAG
  TGCATCACAGACAAGGGATGTTCTCAAACAGCATTTTAATGATTTAAAGGGAACCCTTGGAAAGCTCCTG
  GATGAGCGATTGGTGACCCTTTTGCAAGAGGTGGACACCATTGAACAGGAGACCATTAAACCACTAGATG
  ACTGCCAGAAGCTCATAGAACACGGAGTCAACACTGCAGAGGACTTAGTCCGAGAAGGTGAAATCGCCAT
  GCTTGGTGGTGTGGGAGAAGAGAATGAGAAACTGTGGAGCTTTACCAAAAAGGCCTCGCACATTCAGTTG
  GACAGCTTACCAGAAGTACCTTTACTGGTTGATGTGCCTTGTTTATCTGCTCAGTTGGATGACTCAATTC
  TTAACATAGTGAAAGACCACATTTTTAAGCATGGAACAGTAGCATCTCGCCCACCAGTACAGATAGAAGA
  ACTAATAGAGAAACCTGGAGGCATCATTGTACGATGGTGTAAGGTGGATGATGACTTTACAGCCCAAGAT
  TACAGGCTCCAGTTTCGTAAATGTACTTCAAATCATTTTGAGGATGTATATGTAGGTTCTGAAACTGAAT
  TCATAGTATTGCACATAGACCCCAACGTTGATTACCAGTTCAGAGTCTGCGCCCGAGGAGATGGCCGACA
  GGAGTGGAGTCCTTGGAGTGTCCCCCAGATAGGTCATTCCACATTGGTGCCTCATGAGTGGACAGCTGGT
  TTTGAGGGGTACAGTCTGAGCAGTCGAAGAAATATAGCACTTCGGAACGATTCTGAATCATCGGGTGTTC
  TCTACTCCAGAGCTCCGACTTATTTCTGTGGGCAGACATTAACATTCAGAGTTGAAACTGTGGGACAGCC
  AGACAGAAGAGATAGCATAGGAGTGTGTGCAGAAAAACAGGATGGATATGACTCTCTGCAGCGGGATCAA
  GCTGTGTGCATTAGTACAAATGGTGCAGTTTTTGTCAATGGAAAAGAAATGACAAATCAGTTACCCGCAG
  TTACTTCTGGGTCCACTGTCACGTTTGACATTGAAGCCGTGACTCTAGGAACCACCAGTAATAATGAAGG
  TGGACACTTCAAGCTTCGAGTAACTATAAGTTCAAATAATAGAGAAGTGGTTTTTGACTGGTTACTTGAT
  CAGTCTTGTGGTTCTCTTTACTTTGGATGCTCATTTTTCTATCCTGGATGGAAAGTGTTAGTGTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204326 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRGAMELEPELLLQEARENVEAAQSYRRELGHRLEGLREARRQIKESASQTRDVLKQHFNDLKGTLGKLL
  DERLVTLLQEVDTIEQETIKPLDDCQKLIEHGVNTAEDLVREGEIAMLGGVGEENEKLWSFTKKASHIQL
  DSLPEVPLLVDVPCLSAQLDDSILNIVKDHIFKHGTVASRPPVQIEELIEKPGGIIVRWCKVDDDFTAQD
  YRLQFRKCTSNHFEDVYVGSETEFIVLHIDPNVDYQFRVCARGDGRQEWSPWSVPQIGHSTLVPHEWTAG
  FEGYSLSSRRNIALRNDSESSGVLYSRAPTYFCGQTLTFRVETVGQPDRRDSIGVCAEKQDGYDSLQRDQ
  AVCISTNGAVFVNGKEMTNQLPAVTSGSTVTFDIEAVTLGTTSNNEGGHFKLRVTISSNNREVVFDWLLD
  QSCGSLYFGCSFFYPGWKVLVF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015986
• ORF Size: 1326 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015986.2, NM_015986.3, NP_057070.2, NP_057070.3
• RefSeq Size: 2954 bp
• RefSeq ORF: 1329 bp
• Locus ID: 51379
• Domains: FN3
• Protein Families: Druggable Genome
• MW: 49.8 kDa
• Gene Summary: This gene encodes a cytokine receptor-like factor that may negatively regulate cell cycle progression at the G0/G1 phase. Studies of the related rat protein suggest that it may regulate neuronal morphology and synaptic vesicle biogenesis. This gene is one of several genes located in the neurofibromatosis type I tumor suppressor region on the q arm of chromosome 17, a region that is subject to microdeletions, duplications, chromosomal breaks and rearrangements. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 2 and 5. [provided by RefSeq, Aug 2012]