CRLF3 (NM_015986) Human Tagged ORF Clone

CAT#: RG204326

CRLF3 (GFP-tagged) - Human cytokine receptor-like factor 3 (CRLF3)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CRLF3
• Synonyms: CREME-9; CREME9; CRLM9; CYTOR4; FRWS; p48.2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG204326 representing NM_015986
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGGGGGCGATGGAGCTGGAGCCTGAGCTGCTGTTGCAGGAGGCCCGCGAGAACGTGGAGGCAGCGC
  AGAGCTACCGGCGGGAGCTGGGTCACCGGCTTGAGGGGCTGCGTGAGGCGCGGAGGCAGATCAAAGAAAG
  TGCATCACAGACAAGGGATGTTCTCAAACAGCATTTTAATGATTTAAAGGGAACCCTTGGAAAGCTCCTG
  GATGAGCGATTGGTGACCCTTTTGCAAGAGGTGGACACCATTGAACAGGAGACCATTAAACCACTAGATG
  ACTGCCAGAAGCTCATAGAACACGGAGTCAACACTGCAGAGGACTTAGTCCGAGAAGGTGAAATCGCCAT
  GCTTGGTGGTGTGGGAGAAGAGAATGAGAAACTGTGGAGCTTTACCAAAAAGGCCTCGCACATTCAGTTG
  GACAGCTTACCAGAAGTACCTTTACTGGTTGATGTGCCTTGTTTATCTGCTCAGTTGGATGACTCAATTC
  TTAACATAGTGAAAGACCACATTTTTAAGCATGGAACAGTAGCATCTCGCCCACCAGTACAGATAGAAGA
  ACTAATAGAGAAACCTGGAGGCATCATTGTACGATGGTGTAAGGTGGATGATGACTTTACAGCCCAAGAT
  TACAGGCTCCAGTTTCGTAAATGTACTTCAAATCATTTTGAGGATGTATATGTAGGTTCTGAAACTGAAT
  TCATAGTATTGCACATAGACCCCAACGTTGATTACCAGTTCAGAGTCTGCGCCCGAGGAGATGGCCGACA
  GGAGTGGAGTCCTTGGAGTGTCCCCCAGATAGGTCATTCCACATTGGTGCCTCATGAGTGGACAGCTGGT
  TTTGAGGGGTACAGTCTGAGCAGTCGAAGAAATATAGCACTTCGGAACGATTCTGAATCATCGGGTGTTC
  TCTACTCCAGAGCTCCGACTTATTTCTGTGGGCAGACATTAACATTCAGAGTTGAAACTGTGGGACAGCC
  AGACAGAAGAGATAGCATAGGAGTGTGTGCAGAAAAACAGGATGGATATGACTCTCTGCAGCGGGATCAA
  GCTGTGTGCATTAGTACAAATGGTGCAGTTTTTGTCAATGGAAAAGAAATGACAAATCAGTTACCCGCAG
  TTACTTCTGGGTCCACTGTCACGTTTGACATTGAAGCCGTGACTCTAGGAACCACCAGTAATAATGAAGG
  TGGACACTTCAAGCTTCGAGTAACTATAAGTTCAAATAATAGAGAAGTGGTTTTTGACTGGTTACTTGAT
  CAGTCTTGTGGTTCTCTTTACTTTGGATGCTCATTTTTCTATCCTGGATGGAAAGTGTTAGTGTTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG204326 representing NM_015986
  Red=Cloning site Green=Tags(s)
  
  MRGAMELEPELLLQEARENVEAAQSYRRELGHRLEGLREARRQIKESASQTRDVLKQHFNDLKGTLGKLL
  DERLVTLLQEVDTIEQETIKPLDDCQKLIEHGVNTAEDLVREGEIAMLGGVGEENEKLWSFTKKASHIQL
  DSLPEVPLLVDVPCLSAQLDDSILNIVKDHIFKHGTVASRPPVQIEELIEKPGGIIVRWCKVDDDFTAQD
  YRLQFRKCTSNHFEDVYVGSETEFIVLHIDPNVDYQFRVCARGDGRQEWSPWSVPQIGHSTLVPHEWTAG
  FEGYSLSSRRNIALRNDSESSGVLYSRAPTYFCGQTLTFRVETVGQPDRRDSIGVCAEKQDGYDSLQRDQ
  AVCISTNGAVFVNGKEMTNQLPAVTSGSTVTFDIEAVTLGTTSNNEGGHFKLRVTISSNNREVVFDWLLD
  QSCGSLYFGCSFFYPGWKVLVF
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015986
• ORF Size: 1326 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_015986.2, NP_057070.2
• RefSeq Size: 2848
• RefSeq ORF: 1329
• Locus ID: 51379
• Domains: FN3
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a cytokine receptor-like factor that may negatively regulate cell cycle progression at the G0/G1 phase. Studies of the related rat protein suggest that it may regulate neuronal morphology and synaptic vesicle biogenesis. This gene is one of several genes located in the neurofibromatosis type I tumor suppressor region on the q arm of chromosome 17, a region that is subject to microdeletions, duplications, chromosomal breaks and rearrangements. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 2 and 5. [provided by RefSeq, Aug 2012]