CHIC2 (NM_012110) Human Tagged ORF Clone

CAT#: RC204420

CHIC2 (Myc-DDK-tagged)-Human cysteine-rich hydrophobic domain 2 (CHIC2)

Product Images

Western blot validation of overexpression lysate (Cat# LY415979) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204420 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CHIC2 protein (Cat# TP304420). The protein was produced from HEK293T cells transfected with CHIC2 cDNA clone (Cat# RC204420) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CHIC2
• Synonyms: BTL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204420 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGATTTCGACGAAATCTATGAGGAAGAGGAGGACGAGGAGCGGGCCCTGGAGGAGCAGCTGCTCA
  AGTACTCGCCGGACCCGGTGGTCGTCCGCGGCTCCGGTCACGTCACCGTATTTGGACTGAGCAACAAATT
  TGAATCTGAATTCCCTTCTTCATTAACTGGAAAAGTAGCTCCTGAAGAATTTAAAGCCAGCATCAACAGA
  GTTAACAGTTGTCTTAAGAAGAACCTTCCTGTTAATGTACGTTGGCTACTTTGTGGCTGCCTTTGTTGCT
  GCTGCACATTAGGTTGCAGTATGTGGCCAGTTATTTGCCTCAGTAAAAGAACACGAAGATCGATTGAGAA
  GTTATTAGAATGGGAAAACAATAGGTTATACCACAAGCTGTGCTTGCATTGGAGACTGAGCAAAAGGAAA
  TGTGAAACGAATAACATGATGGAATATGTCATCCTCATAGAATTTTTACCAAAGACACCGATTTTTCGAC
  CAGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204420 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MADFDEIYEEEEDEERALEEQLLKYSPDPVVVRGSGHVTVFGLSNKFESEFPSSLTGKVAPEEFKASINR
  VNSCLKKNLPVNVRWLLCGCLCCCCTLGCSMWPVICLSKRTRRSIEKLLEWENNRLYHKLCLHWRLSKRK
  CETNNMMEYVILIEFLPKTPIFRPD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012110
• ORF Size: 495 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012110.1, NM_012110.2, NM_012110.3, NP_036242.1
• RefSeq Size: 1178 bp
• RefSeq ORF: 498 bp
• Locus ID: 26511
• Protein Families: Druggable Genome, Transmembrane
• MW: 19.3 kDa
• Gene Summary: This gene encodes a member of the CHIC family of proteins. The encoded protein contains a cysteine-rich hydrophobic (CHIC) motif, and is localized to vesicular structures and the plasma membrane. This gene is associated with some cases of acute myeloid leukemia. [provided by RefSeq, Jul 2008]