LZTR1 (NM_006767) Human Tagged ORF Clone

CAT#: RC204432

LZTR1 (Myc-DDK-tagged)-Human leucine-zipper-like transcription regulator 1 (LZTR1)

Product Images

Western blot validation of overexpression lysate (Cat# LY416434) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204432 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LZTR1
• Synonyms: BTBD29; LZTR-1; NS2; NS10; SWNTS2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204432 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGGACCGGGCAGCACGGGGGGGCAGATCGGGGCTGCGGCCCTGGCAGGCGGCGCGCGGTCCAAGG
  TAGCCCCGAGCGTGGACTTCGACCATAGCTGCTCGGACAGTGTCGAGTACCTGACGCTCAACTTCGGGCC
  CTTCGAAACAGTGCATCGCTGGCGGCGCCTCCCGCCCTGCGACGAGTTCGTGGGTGCCCGGCGCAGCAAA
  CACACAGTGGTGGCCTATAAAGATGCCATTTATGTATTTGGTGGAGACAATGGGAAGACCATGCTCAATG
  ACCTCCTGCGGTTCGATGTGAAAGACTGCTCCTGGTGCAGGGCCTTTACCACTGGGACCCCACCGGCCCC
  CCGTTACCACCACTCGGCCGTCGTCTATGGGAGCAGCATGTTTGTCTTTGGGGGTTACACTGGGGACATT
  TATTCCAATTCTAACTTGAAGAATAAAAACGACCTCTTTGAATACAAGTTTGCAACTGGCCAGTGGACGG
  AGTGGAAAATTGAAGGACGGTTGCCAGTCGCTAGGTCAGCCCATGGGGCCACGGTGTACAGTGACAAGCT
  GTGGATCTTTGCTGGCTATGACGGCAACGCCAGGTTGAATGACATGTGGACAATTGGCCTCCAGGACCGA
  GAGCTCACCTGCTGGGAGGAGGTGGCCCAGAGTGGCGAGATCCCCCCATCTTGCTGCAACTTCCCCGTGG
  CTGTGTGCCGGGACAAGATGTTTGTATTCTCTGGGCAAAGCGGAGCCAAAATAACCAACAACCTCTTCCA
  GTTTGAATTCAAGGACAAGACGTGGACACGCATCCCAACTGAACACCTGCTCCGGGGCTCCCCACCACCC
  CCGCAGCGGCGCTACGGGCATACCATGGTGGCCTTTGACCGCCACCTCTATGTGTTTGGGGGTGCGGCCG
  ACAACACGCTGCCCAACGAGCTGCACTGCTATGACGTGGACTTCCAGACCTGGGAGGTCGTCCAGCCCAG
  CTCCGACAGCGAGGTTGGTGGGGCTGAAGTGCCCGAGCGAGCCTGTGCTTCCGAGGAGGTGCCCACCCTG
  ACCTATGAGGAGCGGGTTGGCTTCAAGAAGTCCCGAGATGTGTTTGGCCTGGACTTTGGCACCACCTCAG
  CCAAGCAGCCCACCCAGCCTGCCTCGGAGCTGCCCAGTGGGAGGCTCTTCCACGCGGCTGCTGTCATCTC
  GGACGCCATGTACATCTTCGGGGGCACGGTGGACAACAACATCCGCAGCGGGGAGATGTACAGGTTCCAG
  TTCTCCTGTTACCCTAAATGCACGCTGCACGAGGACTACGGGCGGCTGTGGGAGAGCCGCCAGTTCTGCG
  ACGTGGAGTTCGTGCTGGGTGAGAAGGAGGAGTGCGTGCAGGGCCACGTAGCCATTGTCACAGCGCGGAG
  CCGCTGGCTTCGCAGGAAGATCACGCAGGCGCGGGAGAGGCTGGCCCAGAAGCTGGAGCAGGAGGCCGCC
  CCAGTTCCCAGGGAGGCCCCCGGCGTGGCTGCTGGTGGGGCCCGGCCGCCCCTGCTGCACGTGGCCATCC
  GGGAGGCCGAGGCCCGGCCCTTCGAGGTGCTCATGCAGTTCCTCTACACCGACAAGATCAAATACCCACG
  GAAAGGCCATGTGGAGGATGTGCTGCTCATCATGGATGTGTACAAACTGGCACTGAGCTTCCAGTTGTGC
  CGTCTGGAGCAGCTGTGCCGCCAGTACATCGAGGCCTCCGTGGACCTGCAGAACGTGCTGGTTGTGTGCG
  AGAGTGCCGCCCGGCTGCAGCTGAGCCAACTCAAGGAGCACTGCCTGAACTTCGTGGTAAAGGAGTCCCA
  CTTCAACCAGGTGATCATGATGAAGGAGTTCGAGCGCCTCTCCTCTCCACTGATAGTGGAGATTGTGCGG
  CGGAAGCAGCAGCCGCCCCCTCGCACTCCCTTGGACCAGCCAGTGGACATTGGCACATCTCTGATCCAGG
  ACATGAAGGCATACCTGGAGGGAGCGGGCGCGGAATTCTGTGACATCACTCTGTTGCTTGACGGGCACCC
  ACGGCCAGCCCACAAGGCTATCCTGGCCGCCCGCTCCAGCTACTTTGAAGCCATGTTCCGGTCCTTCATG
  CCCGAAGATGGGCAGGTGAACATCTCCATCGGGGAGATGGTGCCCAGCAGGCAGGCCTTCGAGTCCATGC
  TGCGCTACATCTACTACGGCGAGGTCAACATGCCGCCCGAGGACTCGCTCTACTTGTTTGCGGCCCCCTA
  CTACTACGGCTTCTACAACAACCGGCTGCAGGCGTACTGCAAGCAGAACCTGGAGATGAACGTGACGGTG
  CAGAACGTGCTGCAGATCCTGGAGGCAGCTGACAAAACGCAGGCACTGGACATGAAGCGGCACTGCCTGC
  ACATCATTGTGCACCAGTTCACCAAGGTCTCCAAGTTGCCCACCCTGCGGTCGCTGAGCCAGCAGCTGCT
  GCTGGACATCATAGACTCCCTGGCCTCCCACATCTCAGACAAGCAGTGCGCAGAGCTGGGCGCCGACATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204432 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAGPGSTGGQIGAAALAGGARSKVAPSVDFDHSCSDSVEYLTLNFGPFETVHRWRRLPPCDEFVGARRSK
  HTVVAYKDAIYVFGGDNGKTMLNDLLRFDVKDCSWCRAFTTGTPPAPRYHHSAVVYGSSMFVFGGYTGDI
  YSNSNLKNKNDLFEYKFATGQWTEWKIEGRLPVARSAHGATVYSDKLWIFAGYDGNARLNDMWTIGLQDR
  ELTCWEEVAQSGEIPPSCCNFPVAVCRDKMFVFSGQSGAKITNNLFQFEFKDKTWTRIPTEHLLRGSPPP
  PQRRYGHTMVAFDRHLYVFGGAADNTLPNELHCYDVDFQTWEVVQPSSDSEVGGAEVPERACASEEVPTL
  TYEERVGFKKSRDVFGLDFGTTSAKQPTQPASELPSGRLFHAAAVISDAMYIFGGTVDNNIRSGEMYRFQ
  FSCYPKCTLHEDYGRLWESRQFCDVEFVLGEKEECVQGHVAIVTARSRWLRRKITQARERLAQKLEQEAA
  PVPREAPGVAAGGARPPLLHVAIREAEARPFEVLMQFLYTDKIKYPRKGHVEDVLLIMDVYKLALSFQLC
  RLEQLCRQYIEASVDLQNVLVVCESAARLQLSQLKEHCLNFVVKESHFNQVIMMKEFERLSSPLIVEIVR
  RKQQPPPRTPLDQPVDIGTSLIQDMKAYLEGAGAEFCDITLLLDGHPRPAHKAILAARSSYFEAMFRSFM
  PEDGQVNISIGEMVPSRQAFESMLRYIYYGEVNMPPEDSLYLFAAPYYYGFYNNRLQAYCKQNLEMNVTV
  QNVLQILEAADKTQALDMKRHCLHIIVHQFTKVSKLPTLRSLSQQLLLDIIDSLASHISDKQCAELGADI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006767
• ORF Size: 2520 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006767.2, NM_006767.3, NP_006758.2
• RefSeq Size: 4332 bp
• RefSeq ORF: 2523 bp
• Locus ID: 8216
• Domains: BTB, Kelch
• Protein Families: Transcription Factors
• MW: 94.7 kDa
• Gene Summary: This gene encodes a member of the BTB-kelch superfamily. Initially described as a putative transcriptional regulator based on weak homology to members of the basic leucine zipper-like family, the encoded protein subsequently has been shown to localize exclusively to the Golgi network where it may help stabilize the Gogli complex. Deletion of this gene may be associated with DiGeorge syndrome. [provided by RefSeq, Jul 2008]