UBE2Q2 (NM_173469) Human Tagged ORF Clone

CAT#: RC204433

UBE2Q2 (Myc-DDK-tagged)-Human ubiquitin-conjugating enzyme E2Q family member 2 (UBE2Q2), transcript variant 1


  "NM_173469" in other vectors (4)

Reconstitution Protocol

USD 420.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol UBE2Q2
Synonyms DKFZp762C143
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC204433 representing NM_173469
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCGTGTCAGGGCTCAAGGCCGAGCTGAAGTTCCTGGCGTCCATCTTCGACAAGAACCACGAGCGAT
TCCGCATCGTCAGTTGGAAGCTGGACGAGCTGCACTGCCAGTTCCTGGTGCCGCAGCAGGGCAGCCCGCA
CTCGCTGCCGCCGCCACTCACGCTCCACTGCAACATCACGGAATCCTATCCATCTTCTTCACCGATATGG
TTTGTGGATTCTGAAGACCCAAATCTGACATCAGTTCTGGAACGTCTAGAAGATACTAAGAACAACAATT
TGCTTCGTCAGCAATTGAAGTGGTTGATATGTGAACTCTGCAGTTTATATAACCTTCCTAAGCACCTGGA
TGTTGAGATGCTAGATCAACCACTACCCACGGGTCAGAATGGGACAACAGAAGAAGTGACTTCAGAAGAA
GAGGAAGAAGAAGAAGAGATGGCTGAAGATATAGAAGACTTAGATCACTATGAGATGAAGGAAGAAGAGC
CTATTAGTGGGAAAAAGTCAGAGGATGAAGGAATTGAAAAAGAAAATTTGGCAATATTAGAGAAAATTAG
GAAGACTCAAAGGCAAGACCATTTAAATGGTGCAGTGTCTGGGTCAGTGCAAGCTTCAGATAGACTTATG
AAAGAGCTCAGGGACATATACAGATCACAGAGTTATAAAACAGGGATTTATTCAGTGGAACTCATAAATG
ACAGTTTATATGACTGGCATGTTAAACTGCAGAAGGTTGACCCTGATAGTCCTTTGCACAGTGATCTTCA
GATCTTAAAAGAAAAAGAAGGCATAGAATATATTTTGCTTAACTTCTCTTTTAAGGATAACTTTCCATTT
GATCCTCCATTTGTTCGAGTGGTGTTACCTGTTCTCTCAGGAGGGTATGTATTGGGTGGAGGAGCATTAT
GTATGGAACTTCTCACAAAACAGGGCTGGAGCAGTGCCTACTCAATAGAATCGGTCATCATGCAAATAAA
TGCCACCTTAGTCAAAGGCAAAGCCAGAGTGCAGTTTGGAGCAAATAAGAATCAATATAATCTAGCAAGA
GCCCAACAATCCTATAATTCCATTGTACAGATACATGAGAAAAATGGCTGGTACACCCCTCCAAAGGAAG
ATGGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC204433 representing NM_173469
Red=Cloning site Green=Tags(s)

MSVSGLKAELKFLASIFDKNHERFRIVSWKLDELHCQFLVPQQGSPHSLPPPLTLHCNITESYPSSSPIW
FVDSEDPNLTSVLERLEDTKNNNLLRQQLKWLICELCSLYNLPKHLDVEMLDQPLPTGQNGTTEEVTSEE
EEEEEEMAEDIEDLDHYEMKEEEPISGKKSEDEGIEKENLAILEKIRKTQRQDHLNGAVSGSVQASDRLM
KELRDIYRSQSYKTGIYSVELINDSLYDWHVKLQKVDPDSPLHSDLQILKEKEGIEYILLNFSFKDNFPF
DPPFVRVVLPVLSGGYVLGGGALCMELLTKQGWSSAYSIESVIMQINATLVKGKARVQFGANKNQYNLAR
AQQSYNSIVQIHEKNGWYTPPKEDG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_173469
ORF Size 1125 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_173469.1, NM_173469.2, NM_173469.3, NP_775740.1
RefSeq Size 2939 bp
RefSeq ORF 1128 bp
Locus ID 92912
Protein Pathways Ubiquitin mediated proteolysis
MW 42.6 kDa

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