CD84 (NM_003874) Human Tagged ORF Clone

CAT#: RC204477

CD84 (Myc-DDK-tagged)-Human CD84 molecule (CD84), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY401277) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204477 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CD84 protein (Cat# TP304477). The protein was produced from HEK293T cells transfected with CD84 cDNA clone (Cat# RC204477) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CD84
• Synonyms: hCD84; LY9B; mCD84; SLAMF5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204477 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCAGCACCACCTATGGATCTTGCTCCTTTGCCTGCAAACCTGGCCGGAAGCAGCTGGAAAAGACT
  CAGAAATCTTCACAGTGAATGGGATTCTGGGAGAGTCAGTCACTTTCCCTGTAAATATCCAAGAACCACG
  GCAAGTTAAAATCATTGCTTGGACTTCTAAAACATCTGTTGCTTATGTAACACCAGGAGACTCAGAAACA
  GCACCCGTAGTTACTGTGACCCACAGAAATTATTATGAACGGATACATGCCTTAGGTCCGAACTACAATC
  TGGTCATTAGCGATCTGAGGATGGAAGACGCAGGAGACTACAAAGCAGACATAAATACACAGGCTGATCC
  CTACACCACCACCAAGCGCTACAACCTGCAAATCTATCGTCGGCTTGGGAAACCAAAAATTACACAGAGT
  TTAATGGCATCTGTGAACAGCACCTGTAATGTCACACTGACATGCTCTGTAGAGAAAGAAGAAAAGAATG
  TGACATACAATTGGAGTCCCCTGGGAGAAGAGGGTAATGTCCTTCAAATCTTCCAGACTCCTGAGGACCA
  AGAGCTGACTTACACGTGTACAGCCCAGAACCCTGTCAGCAACAATTCTGACTCCATCTCTGCCCGGCAG
  CTCTGTGCAGACATCGCAATGGGCTTCCGTACTCACCACACCGGGTTGCTGAGCGTGCTGGCTATGTTCT
  TTCTGCTTGTTCTCATTCTGTCTTCAGTGTTTTTGTTCCGTTTGTTCAAGAGAAGACAAGATGCTGCCTC
  AAAGAAAACCATATACACATATATCATGGCTTCAAGGAACACCCAGCCAGCAGAGTCCAGAATCTATGAT
  GAAATCCTGCAGTCCAAGGTGCTTCCCTCCAAGGAAGAGCCAGTGAACACAGTTTATTCCGAAGTGCAGT
  TTGCTGATAAGATGGGGAAAGCCAGCACACAGGACAGTAAACCTCCTGGGACTTCAAGCTATGAAATTGT
  GATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204477 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAQHHLWILLLCLQTWPEAAGKDSEIFTVNGILGESVTFPVNIQEPRQVKIIAWTSKTSVAYVTPGDSET
  APVVTVTHRNYYERIHALGPNYNLVISDLRMEDAGDYKADINTQADPYTTTKRYNLQIYRRLGKPKITQS
  LMASVNSTCNVTLTCSVEKEEKNVTYNWSPLGEEGNVLQIFQTPEDQELTYTCTAQNPVSNNSDSISARQ
  LCADIAMGFRTHHTGLLSVLAMFFLLVLILSSVFLFRLFKRRQDAASKKTIYTYIMASRNTQPAESRIYD
  EILQSKVLPSKEEPVNTVYSEVQFADKMGKASTQDSKPPGTSSYEIVI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003874
• ORF Size: 984 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003874.1, NM_003874.2, NM_003874.3, NP_003865.1
• RefSeq Size: 8245 bp
• RefSeq ORF: 987 bp
• Locus ID: 8832
• Domains: IG
• Protein Families: Druggable Genome, Transmembrane
• MW: 36.9 kDa
• Gene Summary: This gene encodes a membrane glycoprotein that is a member of the signaling lymphocyte activation molecule (SLAM) family. This family forms a subset of the larger CD2 cell-surface receptor Ig superfamily. The encoded protein is a homophilic adhesion molecule that is expressed in numerous immune cells types and is involved in regulating receptor-mediated signaling in those cells. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Oct 2011]