PP2A-beta (PPP2CB) (NM_001009552) Human Tagged ORF Clone
CAT#: RC204491
PPP2CB (Myc-DDK-tagged)-Human protein phosphatase 2, catalytic subunit, beta isozyme (PPP2CB)
"NM_001009552" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | PPP2CB |
Synonyms | PP2Abeta; PP2CB |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC204491 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGACAAGGCGTTCACCAAGGAGCTGGACCAGTGGGTCGAGCAGCTGAACGAGTGTAAGCAGCTGA ACGAGAACCAAGTGCGGACGCTGTGCGAGAAGGCAAAGGAAATTTTAACAAAAGAATCAAATGTGCAAGA GGTTCGTTGCCCTGTTACTGTCTGTGGAGATGTGCATGGTCAATTTCATGATCTTATGGAACTCTTTAGA ATTGGTGGAAAATCACCGGATACAAACTACTTATTCATGGGTGACTATGTAGACAGAGGATATTATTCAG TGGAGACTGTGACTCTTCTTGTAGCATTAAAGGTGCGTTATCCAGAACGCATTACAATATTGAGAGGAAA TCACGAAAGCCGACAAATTACCCAAGTATATGGCTTTTATGATGAATGTCTGCGAAAGTATGGGAATGCC AACGTTTGGAAATATTTTACAGATCTCTTTGATTATCTTCCACTTACAGCTTTAGTAGATGGACAGATAT TCTGCCTCCATGGTGGCCTCTCTCCATCCATAGACACACTGGATCATATAAGAGCCCTGGATCGTTTACA GGAAGTTCCACATGAGGGCCCAATGTGTGATCTGTTATGGTCAGATCCAGATGATCGTGGTGGATGGGGT ATTTCACCACGTGGTGCTGGCTACACATTTGGACAAGACATTTCTGAAACCTTTAACCATGCCAATGGTC TCACACTGGTTTCTCGTGCCCACCAGCTTGTAATGGAGGGATACAATTGGTGTCATGATCGGAATGTGGT TACCATTTTCAGTGCACCCAATTACTGTTATCGTTGTGGGAACCAGGCTGCTATCATGGAATTAGATGAC ACTTTAAAATATTCCTTCCTTCAATTTGACCCAGCGCCTCGTCGTGGTGAGCCTCATGTTACACGGCGCA CCCCAGACTACTTCCTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204491 protein sequence
Red=Cloning site Green=Tags(s) MDDKAFTKELDQWVEQLNECKQLNENQVRTLCEKAKEILTKESNVQEVRCPVTVCGDVHGQFHDLMELFR IGGKSPDTNYLFMGDYVDRGYYSVETVTLLVALKVRYPERITILRGNHESRQITQVYGFYDECLRKYGNA NVWKYFTDLFDYLPLTALVDGQIFCLHGGLSPSIDTLDHIRALDRLQEVPHEGPMCDLLWSDPDDRGGWG ISPRGAGYTFGQDISETFNHANGLTLVSRAHQLVMEGYNWCHDRNVVTIFSAPNYCYRCGNQAAIMELDD TLKYSFLQFDPAPRRGEPHVTRRTPDYFL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001009552 |
ORF Size | 927 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001009552.1, NP_001009552.1 |
RefSeq Size | 1987 bp |
RefSeq ORF | 930 bp |
Locus ID | 5516 |
Cytogenetics | 8p12 |
Protein Families | Druggable Genome, Phosphatase |
Protein Pathways | Long-term depression, Oocyte meiosis, TGF-beta signaling pathway, Tight junction, Wnt signaling pathway |
MW | 35.6 kDa |
Gene Summary | 'This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes a beta isoform of the catalytic subunit. [provided by RefSeq, Mar 2010]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC127985 | PPP2CB (untagged)-Human protein phosphatase 2, catalytic subunit, beta isozyme (PPP2CB) |
USD 660.00 |
|
RG204491 | PPP2CB (GFP-tagged) - Human protein phosphatase 2, catalytic subunit, beta isozyme (PPP2CB) |
USD 460.00 |
|
RC204491L1 | Lenti ORF clone of Human protein phosphatase 2, catalytic subunit, beta isozyme (PPP2CB), Myc-DDK-tagged |
USD 768.00 |
|
RC204491L2 | Lenti ORF clone of Human protein phosphatase 2, catalytic subunit, beta isozyme (PPP2CB), mGFP tagged |
USD 620.00 |
|
RC204491L3 | Lenti ORF clone of Human protein phosphatase 2, catalytic subunit, beta isozyme (PPP2CB), Myc-DDK-tagged |
USD 620.00 |
|
RC204491L4 | Lenti ORF clone of Human protein phosphatase 2, catalytic subunit, beta isozyme (PPP2CB), mGFP tagged |
USD 620.00 |
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