PP2A-beta (PPP2CB) (NM_001009552) Human Untagged Clone

CAT#: SC127985

PPP2CB (untagged)-Human protein phosphatase 2, catalytic subunit, beta isozyme (PPP2CB)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PPP2CB
• Synonyms: PP2Abeta; PP2CB
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001009552, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGACGACAAGGCGTTCACCAAGGAGCTGGACCAGTGGGTCGAGCAGCTGAACGAGTGTAAGCAGCTGA
  ACGAGAACCAAGTGCGGACGCTGTGCGAGAAGGCAAAGGAAATTTTAACAAAAGAATCAAATGTGCAAGA
  GGTTCGTTGCCCTGTTACTGTCTGTGGAGATGTGCATGGTCAATTTCATGATCTTATGGAACTCTTTAGA
  ATTGGTGGAAAATCACCGGATACAAACTACTTATTCATGGGTGACTATGTAGACAGAGGATATTATTCAG
  TGGAGACTGTGACTCTTCTTGTAGCATTAAAGGTGCGTTATCCAGAACGCATTACAATATTGAGAGGAAA
  TCACGAAAGCCGACAAATTACCCAAGTATATGGCTTTTATGATGAATGTCTGCGAAAGTATGGGAATGCC
  AACGTTTGGAAATATTTTACAGATCTCTTTGATTATCTTCCACTTACAGCTTTAGTAGATGGACAGATAT
  TCTGCCTCCATGGTGGCCTCTCTCCATCCATAGACACACTGGATCATATAAGAGCCCTGGATCGTTTACA
  GGAAGTTCCACATGAGGGCCCAATGTGTGATCTGTTATGGTCAGATCCAGATGATCGTGGTGGATGGGGT
  ATTTCACCACGTGGTGCTGGCTACACATTTGGACAAGACATTTCTGAAACCTTTAACCATGCCAATGGTC
  TCACACTGGTTTCTCGTGCCCACCAGCTTGTAATGGAGGGATACAATTGGTGTCATGATCGGAATGTGGT
  TACCATTTTCAGTGCACCCAATTACTGTTATCGTTGTGGGAACCAGGCTGCTATCATGGAATTAGATGAC
  ACTTTAAAATATTCCTTCCTTCAATTTGACCCAGCGCCTCGTCGTGGTGAGCCTCATGTTACACGGCGCA
  CCCCAGACTACTTCCTATAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001009552 unedited
  CCCCCCAGTCAGGGATTTTGTATACGACTCACTATAGGCGGCCGCGAAATTCGGCACGAG
  GCTGCGCGCTCGGAGCCGCCTGCTGGGCTTGGGCGGGGCGCGGGGCCCGCGGCCGCCCTA
  CCCGGCTCAGTCCTCCCCCTGTGGGACCTGGCGACGGCGGCGGAGGGAGAGGGGAGCGGC
  GCCCGGGCCGGGGCCGGGGGCGGGTGGGGAGGGGGGAGGGCGGTGGACGGGCTGGGGCTT
  GGGATCCGCATCGGGATCGGGCCGACATGGACGACAAGGCGTTCACCAAGGAGCTGGACC
  AGTGGGTCGAGCAGCTGAACGAGTGTAAGCAGCTGAACGAGAACCAAGTGCGGACGCTGT
  GCGAGAAGGCAAAGGAAATTTTAACAAAAGAATCAAATGTGCAAGAGGTTCGTTGCCCTG
  TTACTGTCTGTGGAGATGTGCATGGACAATTTCATGACCTTATGGAACTCTTTAGAATTG
  GTGGAAAATCACCGGATACAAACTACTTATTCATGGGTGACTATGTTGACAGAGGATATT
  ATTCAGTGGAGACTGCGACTCTTATTGCAGCATTAAAGGTGTGTTATCCAGAACGCATTA
  CAATATTGAGAGGAAATTACGAAAGCCGACAAACTACCCACGTATATGGCTTTTATGACG
  AATGCCTGCCAAAGTATGGGAAAGCCAATGTTTGGAAATATCTTACAGATCCTTTTGAAT
  ATATTTCAATTCCAGTTTTAACAAAAGGACAAACATTTTGCCTTCAGGTGGCCTATATCA
  ATTCATAAACACACTGCATCATTAAAAAACTCTGGAATGTTACAGGCAGTTCTACATGAG
  GGACCAATGGGGGGACCGTCAAGGGCCTACCCAAAAAAACCGAGTGCCCG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001009552 unedited
  TCACTGGTGGAAGGGAGTCACAGGGCATGCCACCCGGGATCTGTTCAGGAAAAGCTATGA
  CCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGTTTAAAATGAAAAGTTT
  ATTTGCTGAGTACACCAAATAGGATGCAAGCACTGTCATGACAAATATACAGAAATATGC
  AGATCAACATAAAACAGTAATTTAGTTTAAATGAAGGGAAATCTCTTTAAATGTTATGAC
  AACATACTCCCAAGAAGAACCTTTTCTTCAGTTAAGTTAATTATACATTTCAATAAAATA
  AAGGATAATGGATTAGTGGAAGTTAGCTTGTGAATTTTTCTTAAAAATAAAACTCCAACT
  CTATTAATCCATGCCAGTTAAACACTATAACTAAAATTTCCAAATAAGCGCAAAAGGAGA
  TGAAGCAGTTAGTTACCTTTTTTGCTTGAACAGTCCAAAGGAAAATGGTTACTATAAATA
  CAGCAGGCAAACTGTTAGACTGACCTAGAACATAGTGTACTAAATTTCAGTCTCAAATTG
  TGCTAAATGCTCATCATTAGTATGGCACATTTGGTCCATGATGTGGTTTAATGCCAAGAC
  AGATCCCAATTTGTTACAGAAACACATAGATTACTGTTGCTTTTTGTTTTTAAATACATA
  TATTTTAAAAAGCCAGGTATACTTCCACATACAAAGGCAGGTTTCCCAGGAGAAATTTAT
  AGGAAGTAGTCTGGGGTGCGCCGTGTAACATGAGGCTCACCACGACGAGGCGCTGGGTCA
  AATTGAAGGAAGGAATATTTTAAAGTGTCATCTAATTCCAGATAGCAGNCTGGTTTTCCC
  AACAACGATTACAGTAATTTGGGTGCACNTGAAATG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001009552
• Insert Size: 1800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Pass
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001009552.1, NP_001009552.1
• RefSeq Size: 1987 bp
• RefSeq ORF: 930 bp
• Locus ID: 5516
• Cytogenetics: 8p12
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Long-term depression, Oocyte meiosis, TGF-beta signaling pathway, Tight junction, Wnt signaling pathway
• Gene Summary: 'This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes a beta isoform of the catalytic subunit. [provided by RefSeq, Mar 2010]'