NMUR2 (NM_020167) Human Tagged ORF Clone

CAT#: RC204513

NMUR2 (Myc-DDK-tagged)-Human neuromedin U receptor 2 (NMUR2)

Product Images

Western blot validation of overexpression lysate (Cat# LY402759) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204513 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NMUR2
• Synonyms: FM-4; FM4; NMU-R2; NMU2R; TGR-1; TGR1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204513 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGGGATGGAAAAACTTCAGAATGCTTCCTGGATCTACCAGCAGAAACTAGAAGATCCATTCCAGA
  AACACCTGAACAGCACCGAGGAGTATCTGGCCTTCCTCTGCGGACCTCGGCGCAGCCACTTCTTCCTCCC
  CGTGTCTGTGGTGTATGTGCCAATTTTTGTGGTGGGGGTCATTGGCAATGTCCTGGTGTGCCTGGTGATT
  CTGCAGCACCAGGCTATGAAGACGCCCACCAACTACTACCTCTTCAGCCTGGCGGTCTCTGACCTCCTGG
  TCCTGCTCCTTGGAATGCCCCTGGAGGTCTATGAGATGTGGCGCAACTACCCTTTCTTGTTCGGGCCCGT
  GGGCTGCTACTTCAAGACGGCCCTCTTTGAGACCGTGTGCTTCGCCTCCATCCTCAGCATCACCACCGTC
  AGCGTGGAGCGCTACGTGGCCATCCTACACCCGTTCCGCGCCAAACTGCAGAGCACCCGGCGCCGGGCCC
  TCAGGATCCTCGGCATCGTCTGGGGCTTCTCCGTGCTCTTCTCCCTGCCCAACACCAGCATCCATGGCAT
  CAAGTTCCACTACTTCCCCAATGGGTCCCTGGTCCCAGGTTCGGCCACCTTTACGGTCATCAAGCCCATG
  TGGATCTACAATTTCATCATCCAGGTCACCTCCTTCCTATTCTACCTCCTCCCCATGACTGTCATCAGTG
  TCCTCTACTACCTCATGGCACTCAGACTAAAGAAAGACAAATCTCTTGAGGCAGATGAAGGGAATGCAAA
  TATTCAAAGACCCTGCAGAAAATCAGTCAACAAGATGCTGTTTGTCTTGGTCTTAGTGTTTGCTATCTGT
  TGGGCCCCGTTCCACATTGACCGACTCTTCTTCAGCTTTGTGGAGGAGTGGAGTGAATCCCTGGCTGCTG
  TGTTCAACCTCGTCCATGTGGTGTCAGGTGTCTTCTTCTACCTGAGCTCAGCTGTCAACCCCATTATCTA
  TAACCTACTGTCTCGCCGCTTCCAGGCAGCATTCCAGAATGTGATCTCTTCTTTCCACAAACAGTGGCAC
  TCCCAGCATGACCCACAGTTGCCACCTGCCCAGCGGAACATCTTCCTGACAGAATGCCACTTTGTGGAGC
  TGACCGAAGATATAGGTCCCCAATTCCCATGTCAGTCATCCATGCACAACTCTCACCTCCCAACAGCCCT
  CTCTAGTGAACAGATGTCAAGAACAAACTATCAAAGCTTCCACTTTAACAAAACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204513 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSGMEKLQNASWIYQQKLEDPFQKHLNSTEEYLAFLCGPRRSHFFLPVSVVYVPIFVVGVIGNVLVCLVI
  LQHQAMKTPTNYYLFSLAVSDLLVLLLGMPLEVYEMWRNYPFLFGPVGCYFKTALFETVCFASILSITTV
  SVERYVAILHPFRAKLQSTRRRALRILGIVWGFSVLFSLPNTSIHGIKFHYFPNGSLVPGSATFTVIKPM
  WIYNFIIQVTSFLFYLLPMTVISVLYYLMALRLKKDKSLEADEGNANIQRPCRKSVNKMLFVLVLVFAIC
  WAPFHIDRLFFSFVEEWSESLAAVFNLVHVVSGVFFYLSSAVNPIIYNLLSRRFQAAFQNVISSFHKQWH
  SQHDPQLPPAQRNIFLTECHFVELTEDIGPQFPCQSSMHNSHLPTALSSEQMSRTNYQSFHFNKT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020167
• ORF Size: 1245 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020167.2, NM_020167.3, NM_020167.4, NP_064552.2, NP_064552.3
• RefSeq Size: 2067 bp
• RefSeq ORF: 1248 bp
• Locus ID: 56923
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• MW: 47.8 kDa
• Gene Summary: This gene encodes a protein from the G-protein coupled receptor 1 family. This protein is a receptor for neuromedin U, which is a neuropeptide that is widely distributed in the gut and central nervous system. This receptor plays an important role in the regulation of food intake and body weight. [provided by RefSeq, Jul 2008]