TH1L (NELFCD) (NM_198976) Human Tagged ORF Clone

CAT#: RC204842

NELFCD (Myc-DDK-tagged)-Human TH1-like (Drosophila) (TH1L), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY404699) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204842 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified NELFCD protein (Cat# TP304842). The protein was produced from HEK293T cells transfected with NELFCD cDNA clone (Cat# RC204842) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NELFCD
• Synonyms: HSPC130; NELF-C; NELF-D; TH1; TH1L
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204842 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGGGCCGTGCCGGGCGCCATCATGGACGAGGACTACTACGGGAGCGCGGCCGAGTGGGGCGACG
  AGGCTGACGGCGGCCAGCAGGAGGATGATTCTGGAGAAGGAGAGGATGATGCGGAGGTTCAGCAAGAATG
  CCTGCATAAATTTTCCACCCGGGATTATATCATGGAACCCTCCATCTTCAACACTCTGAAGAGGTATTTT
  CAGGCAGGAGGGTCTCCAGAGAATGTTATCCAGCTCTTATCTGAAAACTACACCGCTGTGGCCCAGACTG
  TGAACCTGCTGGCCGAGTGGCTCATTCAGACAGGTGTTGAGCCAGTGCAGGTTCAGGAAACTGTGGAAAA
  TCACTTGAAGAGTTTGCTGATCAAACATTTTGACCCCCGCAAAGCAGATTCTATTTTTACTGAAGAAGGA
  GAGACCCCAGCGTGGCTGGAACAGATGATTGCACATACCACGTGGCGGGACCTTTTTTATAAACTGGCTG
  AAGCCCATCCAGACTGTTTGATGCTGAACTTCACCGTTAAGCTTATTTCTGACGCAGGGTACCAGGGGGA
  GATCACCAGTGTGTCCACAGCATGCCAGCAGCTAGAAGTGTTCTCGAGAGTGCTCCGGACCTCTCTAGCT
  ACAATTTTAGATGGAGGAGAAGAAAACCTTGAAAAAAATCTCCCTGAGTTTGCTAAGATGGTGTGCCACG
  GGGAGCACACGTACCTGTTTGCCCAGGCCATGATGTCCGTGCTGGCCCAGGAGGAGCAGGGGGGCTCCGC
  TGTGCGCAGGATCGCCCAGGAAGTGCAGCGCTTTGCCCAGGAGAAAGGTCATGACGCCAGTCAGATCACA
  CTAGCCTTGGGCACAGCTGCCTCCTACCCCAGGGCCTGCCAGGCTCTCGGGGCCATGCTGTCCAAAGGAG
  CCCTGAACCCTGCTGACATCACCGTCCTGTTCAAGATGTTCACAAGCATGGACCCTCCTCCGGTTGAACT
  TATCCGCGTTCCAGCCTTCCTGGACCTGTTCATGCAGTCACTCTTTAAACCAGGGGCTCGGATCAACCAG
  GACCACAAGCACAAATACATCCACATCTTGGCGTACGCAGCAAGCGTGGTTGAGACCTGGAAGAAGAACA
  AGCGAGTGAGCATCAATAAAGATGAGCTGAAGTCAACGTCAAAAGCTGTCGAAACCGTTCACAATTTGTG
  TTGCAACGAGAACAAAGGGGCCTCTGAACTAGTGGCAGAATTGAGCACACTTTATCAGTGTATTAGGTTT
  CCAGTGGTAGCAATGGGTGTGCTGAAGTGGGTGGATTGGACTGTATCAGAACCAAGGTACTTTCAGCTGC
  AGACTGACCATACCCCTGTCCACCTGGCGTTGCTGGATGAGATCAGCACCTGCCACCAGCTCCTGCACCC
  CCAGGTCCTGCAGCTGCTTGTTAAGCTTTTTGAGACTGAGCACTCCCAGCTGGACGTGATGGAGCAGCTT
  GAGTTGAAGAAGACACTGCTGGACAGGATGGTTCACCTGCTGAGTCGAGGTTATGTACTTCCTGTTGTCA
  GTTACATCCGAAAGTGTCTGGAGAAGCTGGACACTGACATTTCACTCATTCGCTATTTTGTCACTGAGGT
  GCTGGACGTCATTGCTCCTCCTTATACCTCTGACTTCGTGCAACTTTTCCTCCCCATCCTGGAGAATGAC
  AGCATCGCAGGTACCATCAAAACGGAAGGCGAGCATGACCCTGTGACGGAGTTTATAGCTCACTGCAAAT
  CTAACTTCATCATGGTGAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204842 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAGAVPGAIMDEDYYGSAAEWGDEADGGQQEDDSGEGEDDAEVQQECLHKFSTRDYIMEPSIFNTLKRYF
  QAGGSPENVIQLLSENYTAVAQTVNLLAEWLIQTGVEPVQVQETVENHLKSLLIKHFDPRKADSIFTEEG
  ETPAWLEQMIAHTTWRDLFYKLAEAHPDCLMLNFTVKLISDAGYQGEITSVSTACQQLEVFSRVLRTSLA
  TILDGGEENLEKNLPEFAKMVCHGEHTYLFAQAMMSVLAQEEQGGSAVRRIAQEVQRFAQEKGHDASQIT
  LALGTAASYPRACQALGAMLSKGALNPADITVLFKMFTSMDPPPVELIRVPAFLDLFMQSLFKPGARINQ
  DHKHKYIHILAYAASVVETWKKNKRVSINKDELKSTSKAVETVHNLCCNENKGASELVAELSTLYQCIRF
  PVVAMGVLKWVDWTVSEPRYFQLQTDHTPVHLALLDEISTCHQLLHPQVLQLLVKLFETEHSQLDVMEQL
  ELKKTLLDRMVHLLSRGYVLPVVSYIRKCLEKLDTDISLIRYFVTEVLDVIAPPYTSDFVQLFLPILEND
  SIAGTIKTEGEHDPVTEFIAHCKSNFIMVN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198976
• ORF Size: 1770 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198976.1, NP_945327.1
• RefSeq Size: 2320 bp
• RefSeq ORF: 1746 bp
• Locus ID: 51497
• Protein Families: Transcription Factors
• MW: 66.2 kDa
• Gene Summary: The NELF complex of proteins interacts with the DSIF protein complex to repress transcriptional elongation by RNA polymerase II. The protein encoded by this gene is an essential part of the NELF complex. Alternative translation initiation site usage results in the formation of two isoforms with different N-termini. [provided by RefSeq, Jul 2008]