SCY1 like 3 (SCYL3) (NM_020423) Human Tagged ORF Clone
CAT#: RC204859
SCYL3 (Myc-DDK-tagged)-Human SCY1-like 3 (S. cerevisiae) (SCYL3), transcript variant 1
"NM_020423" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | SCYL3 |
Synonyms | PACE-1; PACE1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC204859 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGATCAGAGAACAGTGCTTTAAAGAGCTATACACTGAGAGAACCACCATTTACCTTACCCTCTGGAC TTGCTGTTTATCCCGCTGTACTGCAAGATGGCAAATTTGCTTCAGTTTTTGTGTATAAGAGAGAAAATGA AGACAAGGTTAATAAAGCTGCCAAGCATTTGAAGACACTTCGTCACCCTTGCTTGCTAAGATTTTTATCT TGTACTGTGGAAGCGGATGGCATTCATCTTGTCACTGAGCGAGTACAGCCCCTGGAAGTGGCTTTGGAAA CATTGTCTTCTGCAGAGGTCTGTGCTGGGATCTATGACATATTGCTGGCTCTTATCTTCCTTCATGACAG AGGACACCTAACACACAATAATGTCTGTTTATCATCTGTGTTTGTGAGTGAAGATGGACACTGGAAGCTA GGAGGAATGGAAACTGTTTGTAAAGTTTCTCAGGCCACACCAGAGTTTCTGAGGAGTATTCAGTCAATAA GAGACCCAGCATCTATCCCTCCTGAAGAGATGTCTCCAGAATTCACAACTCTCCCAGAGTGTCATGGACA TGCCCGGGATGCCTTTTCATTTGGAACATTGGTGGAAAGTTTGCTCACAATCTTAAATGAACAGGTTTCA GCGGATGTTCTCTCCAGCTTTCAACAGACCTTGCACTCAACTTTGCTGAATCCCATTCCAAAATGTCGGC CAGCGCTCTGCACCTTACTATCTCATGACTTCTTCAGAAATGATTTTCTGGAAGTTGTGAATTTCTTGAA AAGTTTAACATTGAAGAGTGAAGAGGAGAAAACGGAATTCTTTAAATTTCTGCTGGACAGAGTCAGCTGC TTGTCAGAGGAATTGATAGCTTCAAGGTTGGTGCCTCTTCTGCTTAATCAGTTGGTGTTTGCAGAGCCAG TGGCTGTTAAGAGTTTTCTTCCTTATCTGCTTGGCCCCAAAAAAGATCATGCGCAGGGAGAAACTCCTTG CTTGCTCTCACCAGCCCTGTTCCAGTCACGGGTGATCCCCGTGCTTCTCCAGTTGTTTGAAGTTCATGAA GAGCATGTGCGGATGGTGCTGCTGTCTCACATCGAGGCCTACGTGGAGCACTTCACTCAGGAGCAGCTGA AGAAAGTCATCTTGCCACAGGTTTTGCTGGGCCTGCGTGATACTAGTGATTCCATTGTGGCAATTACTCT GCATAGCCTAGCAGTGCTGGTCTCTCTGCTTGGACCAGAGGTGGTTGTGGGAGGAGAACGAACCAAGATC TTCAAACGCACTGCCCCAAGTTTTACTAAAAATACTGACCTTTCTCTAGAAGGTGATCCATTTTCTCAGC CTATTAAATTTCCCATAAACGGACTCTCAGATGTAAAAAATACTTCGGAGGACAGTGAAAACTTCCCATC AAGTTCTAAAAAGTCTGAGGAGTGGCCTGACTGGAGTGAACCTGAGGAGCCTGAAAATCAAACTGTCAAC ATACAGATTTGGCCTAGAGAACCTTGTGATGATGTCAAGTCCCAGTGCACTACCTTGGATGTGGAAGAGT CATCTTGGGATGACTGCGAGCCCAGCAGCTTAGATACTAAAGTAAACCCAGGAGGTGGAATCACTGCTAC AAAACCTGTTACCTCAGCGGAGCAGAAGCCTATTCCTGCTTTGCTTTCACTCACTGAAGAGTCTATGCCT TGGAAATCAAGCTTACCCCAAAAGATTAGCCTTGTACAAAGGGGGGATGACGCAGACCAAATCGAGCCGC CAAAAGTGTCATCACAAGAAAGGCCCCTTAAGGTTCCATCAGAACTTGGTTTAGGAGAGGAATTCACCAT TCAAGTAAAAAAGAAGCCAGTAAAAGATCCTGAGATGGATTGGTTTGCTGATATGATCCCAGAAATTAAG CCTTCTGCTGCTTTTCTTATATTACCTGAACTGAGGACAGAAATGGTCCCAAAAAAGGATGATGTCTCCC CAGTGATGCAGTTTTCCTCAAAATTTGCTGCAGCAGAAATTACTGAGGGAGAGGCTGAAGGCTGGGAAGA AGAAGGGGAGCTGAACTGGGAAGATAATAACTGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC204859 protein sequence
Red=Cloning site Green=Tags(s) MGSENSALKSYTLREPPFTLPSGLAVYPAVLQDGKFASVFVYKRENEDKVNKAAKHLKTLRHPCLLRFLS CTVEADGIHLVTERVQPLEVALETLSSAEVCAGIYDILLALIFLHDRGHLTHNNVCLSSVFVSEDGHWKL GGMETVCKVSQATPEFLRSIQSIRDPASIPPEEMSPEFTTLPECHGHARDAFSFGTLVESLLTILNEQVS ADVLSSFQQTLHSTLLNPIPKCRPALCTLLSHDFFRNDFLEVVNFLKSLTLKSEEEKTEFFKFLLDRVSC LSEELIASRLVPLLLNQLVFAEPVAVKSFLPYLLGPKKDHAQGETPCLLSPALFQSRVIPVLLQLFEVHE EHVRMVLLSHIEAYVEHFTQEQLKKVILPQVLLGLRDTSDSIVAITLHSLAVLVSLLGPEVVVGGERTKI FKRTAPSFTKNTDLSLEGDPFSQPIKFPINGLSDVKNTSEDSENFPSSSKKSEEWPDWSEPEEPENQTVN IQIWPREPCDDVKSQCTTLDVEESSWDDCEPSSLDTKVNPGGGITATKPVTSAEQKPIPALLSLTEESMP WKSSLPQKISLVQRGDDADQIEPPKVSSQERPLKVPSELGLGEEFTIQVKKKPVKDPEMDWFADMIPEIK PSAAFLILPELRTEMVPKKDDVSPVMQFSSKFAAAEITEGEAEGWEEEGELNWEDNNW TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_020423 |
ORF Size | 2064 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_020423.1, NM_020423.2, NM_020423.3, NM_020423.4, NM_020423.5, NM_020423.6, NP_065156.4 |
RefSeq Size | 2928 bp |
RefSeq ORF | 2067 bp |
Locus ID | 57147 |
Domains | S_TKc |
Protein Families | Druggable Genome, Protein Kinase |
MW | 76.7 kDa |
Gene Summary | This gene encodes a protein with a kinase domain and four HEAT repeats. The encoded protein interacts with the C-terminal domain of ezrin, an ERM protein, and may play a role in cell adhesion and migration. Alternative splicing results in multiple transcript variants encoding multiple isoforms. [provided by RefSeq, Jun 2012] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC113079 | SCYL3 (untagged)-Human SCY1-like 3 (S. cerevisiae) (SCYL3), transcript variant 1 |
USD 310.00 |
|
RG204859 | SCYL3 (GFP-tagged) - Human SCY1-like 3 (S. cerevisiae) (SCYL3), transcript variant 1 |
USD 460.00 |
|
RC204859L1 | Lenti ORF clone of Human SCY1-like 3 (S. cerevisiae) (SCYL3), transcript variant 1, Myc-DDK-tagged |
USD 620.00 |
|
RC204859L2 | Lenti ORF clone of Human SCY1-like 3 (S. cerevisiae) (SCYL3), transcript variant 1, mGFP tagged |
USD 620.00 |
|
RC204859L3 | Lenti ORF clone of Human SCY1-like 3 (S. cerevisiae) (SCYL3), transcript variant 1, Myc-DDK-tagged |
USD 620.00 |
|
RC204859L4 | Lenti ORF clone of Human SCY1-like 3 (S. cerevisiae) (SCYL3), transcript variant 1, mGFP tagged |
USD 620.00 |
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