ZBTB9 (NM_152735) Human Tagged ORF Clone

CAT#: RC204920

ZBTB9 (Myc-DDK-tagged)-Human zinc finger and BTB domain containing 9 (ZBTB9)

Product Images

Western blot validation of overexpression lysate (Cat# LY407333) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204920 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZBTB9
• Synonyms: ZNF919
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204920 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAACCCCAACACCTTTGCCGCCTGTACCCGCCTCCCCGACCTGCAACCCAGCCCCACGGACAATCC
  AGATCGAGTTCCCACAGCATAGCTCGTCGCTGCTGGAATCTCTGAACCGCCACAGGCTAGAGGGAAAGTT
  CTGTGATGTGTCCCTCCTGGTGCAGGGCCGGGAACTTAGGGCTCATAAAGCAGTGTTAGCTGCTGCCTCT
  CCTTACTTCCATGACAAGCTGCTTCTGGGGGATGCGCCTCGTCTCACTCTACCGAGTGTCATTGAAGCCG
  ATGCCTTCGAGGGGCTGCTCCAGCTCATTTATTCAGGGCGTCTCCGCCTGCCACTGGATGCTCTTCCTGC
  TCATCTCCTTGTGGCCAGTGGCCTTCAAATGTGGCAGGTAGTAGATCAGTGCTCAGAAATTCTTAGAGAA
  TTAGAAACTTCAGGTGGTGGAATTTCAGCCCGTGGAGGAAACTCCTACCATGCCCTTCTTTCCACTACAT
  CCTCTACAGGAGGCTGGTGCATTCGCTCTTCGCCTTTCCAGACCCCAGTACAGTCCTCTGCTTCTACTGA
  AAGCCCTGCTTCCACTGAGAGCCCTGTGGGAGGGGAGGGAAGTGAACTGGGAGAAGTGCTGCAAATTCAG
  GTGGAAGAAGAAGAGGAGGAGGAGGAAGATGATGATGATGAGGACCAGGGGTCAGCCACACTCTCTCAGA
  CTCCTCAGCCCCAGAGAGTATCAGGGGTTTTTCCCCGTCCTCATGGACCCCACCCACTGCCCATGACTGC
  TACTCCCCGAAAGCTTCCAGAGGGTGAGAGTGCACCACTTGAGCTTCCTGCCCCTCCTGCACTGCCCCCC
  AAAATCTTCTACATTAAGCAGGAACCCTTCGAGCCTAAGGAGGAGATATCAGGAAGCGGAACTCAGCCTG
  GAGGAGCAAAGGAGGAAACCAAAGTGTTTTCTGGAGGGGACACTGAAGGGAATGGGGAGCTAGGGTTCTT
  GTTGCCTTCAGGGCCAGGGCCAACATCTGGGGGAGGGGGTCCATCCTGGAAACCAGTGGATCTTCATGGG
  AATGAAATCCTGTCAGGGGGTGGAGGACCTGGGGGAGCAGGCCAGGCCGTGCATGGGCCTGTGAAGCTAG
  GGGGGACACCCCCTGCAGATGGAAAACGCTTTGGTTGCCTGTGTGGGAAGCGGTTTGCAGTGAAGCCAAA
  GCGTGACCGGCACATCATGCTGACCTTCAGCCTTCGGCCTTTTGGCTGTGGCATCTGCAACAAGCGCTTC
  AAGCTGAAGCACCATCTGACAGAGCACATGAAGACCCATGCTGGAGCCCTGCATGCCTGTCCCCACTGTG
  GCCGTCGGTTCCGAGTCCATGCCTGTTTTCTCCGCCACCGGGACCTATGCAAGGGCCAGGGCTGGGCCAC
  TGCCCACTGGACTTACAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204920 protein sequence
  Red=Cloning site Green=Tags(s)
  
  METPTPLPPVPASPTCNPAPRTIQIEFPQHSSSLLESLNRHRLEGKFCDVSLLVQGRELRAHKAVLAAAS
  PYFHDKLLLGDAPRLTLPSVIEADAFEGLLQLIYSGRLRLPLDALPAHLLVASGLQMWQVVDQCSEILRE
  LETSGGGISARGGNSYHALLSTTSSTGGWCIRSSPFQTPVQSSASTESPASTESPVGGEGSELGEVLQIQ
  VEEEEEEEEDDDDEDQGSATLSQTPQPQRVSGVFPRPHGPHPLPMTATPRKLPEGESAPLELPAPPALPP
  KIFYIKQEPFEPKEEISGSGTQPGGAKEETKVFSGGDTEGNGELGFLLPSGPGPTSGGGGPSWKPVDLHG
  NEILSGGGGPGGAGQAVHGPVKLGGTPPADGKRFGCLCGKRFAVKPKRDRHIMLTFSLRPFGCGICNKRF
  KLKHHLTEHMKTHAGALHACPHCGRRFRVHACFLRHRDLCKGQGWATAHWTYK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152735
• ORF Size: 1419 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152735.1, NM_152735.2, NM_152735.3, NP_689948.1
• RefSeq Size: 2730 bp
• RefSeq ORF: 1422 bp
• Locus ID: 221504
• Domains: BTB, zf-C2H2
• Protein Families: Transcription Factors
• MW: 50.6 kDa
• Gene Summary: May be involved in transcriptional regulation. [UniProtKB/Swiss-Prot Function]