PCTAIRE1 (CDK16) (NM_033018) Human Tagged ORF Clone

CAT#: RC204962

CDK16 (Myc-DDK-tagged)-Human cyclin-dependent kinase 16 (CDK16), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY409781) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204962 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CDK16 protein (Cat# TP304962). The protein was produced from HEK293T cells transfected with CDK16 cDNA clone (Cat# RC204962) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CDK16
• Synonyms: PCTAIRE; PCTAIRE1; PCTGAIRE; PCTK1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204962 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCGGATGAAGAAGATCAAACGGCAGCTGTCAATGACACTCCGAGGTGGCCGAGGCATAGACAAGA
  CCAATGGTGCCCCTGAGCAGATAGGCCTGGATGAGAGTGGTGGTGGTGGCGGCAGTGACCCTGGAGAGGC
  CCCCACACGTGCTGCTCCTGGGGAACTTCGTTCTGCACGGGGCCCACTCAGCTCTGCACCAGAGATTGTG
  CACGAGGACTTGAAGATGGGGTCTGATGGGGAGAGTGACCAGGCTTCAGCCACGTCCTCGGATGAGGTGC
  AGTCTCCAGTGAGAGTGCGTATGCGCAACCATCCCCCACGCAAGATCTCCACTGAGGACATCAACAAGCG
  CCTATCACTACCAGCTGACATCCGGCTGCCTGAGGGCTACCTGGAGAAGCTGACCCTCAATAGCCCCATC
  TTTGACAAGCCCCTCAGCCGCCGCCTCCGTCGTGTCAGCCTATCTGAGATTGGCTTTGGGAAACTGGAGA
  CCTACATTAAGCTGGACAAACTGGGCGAGGGTACCTATGCCACCGTCTACAAAGGCAAAAGCAAGCTCAC
  AGACAACCTTGTGGCACTCAAGGAGATCAGACTGGAACATGAAGAGGGGGCACCCTGCACCGCCATCCGG
  GAAGTGTCCCTGCTCAAGGACCTCAAACACGCCAACATCGTTACGCTACATGACATTATCCACACGGAGA
  AGTCCCTCACCCTTGTCTTTGAGTACCTGGACAAGGACCTGAAGCAGTACCTGGATGACTGTGGGAACAT
  CATCAACATGCACAACGTGAAACTGTTCCTGTTCCAGCTGCTCCGTGGCCTGGCCTACTGCCACCGGCAG
  AAGGTGCTACACCGAGACCTCAAGCCCCAGAACCTGCTCATCAACGAGAGGGGAGAGCTCAAGCTGGCTG
  ACTTTGGCCTGGCCCGAGCCAAGTCAATCCCAACAAAGACATACTCCAATGAGGTGGTGACACTGTGGTA
  CCGGCCCCCTGACATCCTGCTTGGGTCCACGGACTACTCCACTCAGATTGACATGTGGGGTGTGGGCTGC
  ATCTTCTATGAGATGGCCACAGGCCGTCCCCTCTTTCCGGGCTCCACGGTGGAGGAACAGCTACACTTCA
  TCTTCCGTATCTTAGGAACCCCAACTGAGGAGACGTGGCCAGGCATCCTGTCCAACGAGGAGTTCAAGAC
  ATACAACTACCCCAAGTACCGAGCCGAGGCCCTTTTGAGCCACGCACCCCGACTTGATAGCGACGGGGCC
  GACCTCCTCACCAAGCTGTTGCAGTTTGAGGGTCGAAATCGGATCTCCGCAGAGGATGCCATGAAACATC
  CATTCTTCCTCAGTCTGGGGGAGCGGATCCACAAACTTCCTGACACTACTTCCATATTTGCACTAAAGGA
  GATTCAGCTACAAAAGGAGGCCAGCCTTCGGTCTTCGTCGATGCCTGACTCAGGCAGGCCAGCTTTCCGC
  GTGGTGGACACCGAGTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204962 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDRMKKIKRQLSMTLRGGRGIDKTNGAPEQIGLDESGGGGGSDPGEAPTRAAPGELRSARGPLSSAPEIV
  HEDLKMGSDGESDQASATSSDEVQSPVRVRMRNHPPRKISTEDINKRLSLPADIRLPEGYLEKLTLNSPI
  FDKPLSRRLRRVSLSEIGFGKLETYIKLDKLGEGTYATVYKGKSKLTDNLVALKEIRLEHEEGAPCTAIR
  EVSLLKDLKHANIVTLHDIIHTEKSLTLVFEYLDKDLKQYLDDCGNIINMHNVKLFLFQLLRGLAYCHRQ
  KVLHRDLKPQNLLINERGELKLADFGLARAKSIPTKTYSNEVVTLWYRPPDILLGSTDYSTQIDMWGVGC
  IFYEMATGRPLFPGSTVEEQLHFIFRILGTPTEETWPGILSNEEFKTYNYPKYRAEALLSHAPRLDSDGA
  DLLTKLLQFEGRNRISAEDAMKHPFFLSLGERIHKLPDTTSIFALKEIQLQKEASLRSSSMPDSGRPAFR
  VVDTEF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_033018
• ORF Size: 1488 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033018.1, NM_033018.2, NP_148978.1
• RefSeq Size: 3280 bp
• RefSeq ORF: 1509 bp
• Locus ID: 5127
• Cytogenetics: Xp11.3
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• MW: 55.7 kDa
• Gene Summary: 'The protein encoded by this gene belongs to the cdc2/cdkx subfamily of the ser/thr family of protein kinases. It may play a role in signal transduction cascades in terminally differentiated cells; in exocytosis; and in transport of secretory cargo from the endoplasmic reticulum. This gene is thought to escape X inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2009]'