PCTAIRE1 (CDK16) (NM_033018) Human Untagged Clone

CAT#: SC323582

CDK16 (untagged)-Kinase deficient mutant (K194M) of Human PCTAIRE protein kinase 1 (PCTK1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CDK16
• Synonyms: PCTAIRE; PCTAIRE1; PCTGAIRE; PCTK1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_033018, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCAGTCCGAGATCGCCATGGATCGGATGAAGAAGATCAAACGGCAGCTGTCAATGACACTCCGAGGTG
  GCCGAGGCATAGACAAGACCAATGGTGCCCCTGAGCAGATAGGCCTGGATGAGAGTGGTGGTGGTGGCGG
  CAGTGACCCTGGAGAGGCCCCCACACGTGCTGCTCCTGGGGAACTTCGTTCTGCACGGGGCCCACTCAGC
  TCTGCACCAGAGATTGTGCACGAGGACTTGAAGATGGGGTCTGATGGGGAGAGTGACCAGGCTTCAGCCA
  CGTCCTCGGATGAGGTGCAGTCTCCAGTGAGAGTGCGTATGCGCAACCATCCCCCACGCAAGATCTCCAC
  TGAGGACATCAACAAGCGCCTATCACTACCAGCTGACATCCGGCTGCCTGAGGGCTACCTGGAGAAGCTG
  ACCCTCAATAGCCCCATCTTTGACAAGCCCCTCAGCCGCCGCCTCCGTCGTGTCAGCCTATCTGAGATTG
  GCTTTGGGAAACTGGAGACCTACATTAAGCTGGACAAACTGGGCGAGGGTACCTATGCCACCGTCTACAA
  AGGCAAAAGCAAGCTCACAGACAACCTTGTGGCACTCAAGGAGATCAGACTGGAACATGAAGAGGGGGCA
  CCCTGCACCGCCATCCGGGAAGTGTCCCTGCTCAAGGACCTCAAACACGCCAACATCGTTACGCTACATG
  ACATTATCCACACGGAGAAGTCCCTCACCCTTGTCTTTGAGTACCTGGACAAGGACCTGAAGCAGTACCT
  GGATGACTGTGGGAACATCATCAACATGCACAACGTGAAACTGTTCCTGTTCCAGCTGCTCCGTGGCCTG
  GCCTACTGCCACCGGCAGAAGGTGCTACACCGAGACCTCAAGCCCCAGAACCTGCTCATCAACGAGAGGG
  GAGAGCTCAAGCTGGCTGACTTTGGCCTGGCCCGAGCCAAGTCAATCCCAACAAAGACATACTCCAATGA
  GGTGGTGACACTGTGGTACCGGCCCCCTGACATCCTGCTTGGGTCCACGGACTACTCCACTCAGATTGAC
  ATGTGGGGTGTGGGCTGCATCTTCTATGAGATGGCCACAGGCCGTCCCCTCTTTCCGGGCTCCACGGTGG
  AGGAACAGCTACACTTCATCTTCCGTATCTTAGGAACCCCAACTGAGGAGACGTGGCCAGGCATCCTGTC
  CAACGAGGAGTTCAAGACATACAACTACCCCAAGTACCGAGCCGAGGCCCTTTTGAGCCACGCACCCCGA
  CTTGATAGCGACGGGGCCGACCTCCTCACCAAGCTGTTGCAGTTTGAGGGTCGAAATCGGATCTCCGCAG
  AGGATGCCATGAAACATCCATTCTTCCTCAGTCTGGGGGAGCGGATCCACAAACTTCCTGACACTACTTC
  CATATTTGCACTAAAGGAGATTCAGCTACAAAAGGAGGCCAGCCTTCGGTCTTCGTCGATGCCTGACTCA
  GGCAGGCCAGCTTTCCGCGTGGTGGACACCGAGTTCTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_033018 unedited
  ACCGCCCGTACAGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTTCTATATAAGCAGAGCTCGTTTAGTT
  GAACCGTCAGAATCTTGTAATACGACTCACTATAGGGCGGCCGCGAATACGGCACGAGCCTCCCCAAGCA
  CCTCAAGGGACTAGAACTGAGCGCTTCATTTGTCTTTTTTCCTCCTTGCAAAAGTCCCGTATGCCACCAT
  GGGGATGTACCAAGTGAGACCGAGTAGGGGGAACGAGTGGTGATTGACGCGCCAGGTTACTGGCCACTGC
  TCACCTAGGCGCTAGCAAACTTTCTGCCAAGATCGGGAACTGAGTACTAAACAGCCCTCCACAGTTTCTC
  CCTGGTGCCGTCTCCGGCTTGGCGCCGCATCCTCCCTCTGGGCTTCGCGATTGCCCGCGTCCCCCTCCCC
  GCTGCGCGGAACGGGTTCCTTTTGTTCACATGCGCAGTCCGAGAAATCCGCCTATGATATCCGAAATGAA
  AAAGATATCCAAACGGCAAGCTGTCATTGACCCTCCCCAGAGGTGGGCCAGAGGCTAAAAACAAGAACCA
  ATGGTCCCCTGGACCAAATAGGCCTGATTAAAATTGGTGGGGGGGTGGGCGAATGTCACCCGGGGAAGAG
  CCCCCCACCTGTGTGGGCCCGGGGGGAAATTTGTTTGCGCCGGGGGCCCCTCTCCTCTGTACCCAAAAAT
  TTGCCCCAAGAAGTTTTGAATAATGTCTTCTAATGAAAAGATAAACCCTCTTACCCGTGTCTGAGAAGAG
  GTCGATCCCTGAGAGAGCTTATGCGCCCCATCTCCCGCCGATTCTCCTGAGGGATATTAACGCCCATATA
  CTCACTGCATCTCTGGCTCGAGAGCTCGTGAAAGACTCACTATACCCTCTTGATGAACCCCCTGCGCCCG
  TCCGTTGGCACATTTAGATAGCTGTGAGAGCGACACTCAATTAGCAACAGCGAGGAGGATTACGGCTTCA
  AGAGAGGAGTCACT
  
  Kinase domain sequence

  >SC323582 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  TSTGGCAGCTGGGCGAGGGTACCTATGCCRCCGTCKACAAAGGCRAAAGCAAGCTCACAGACAACCTTGT
  GGCACTCATGGAGATCAGACTGGAACATGAAGAGGGGGCACCCTGCACCGCCATCCGGGAAGTGTCCCTG
  CTCAAGGACCTCAAACACGCCAACATCGTTACGCTACATGACATTATCCACACGGAGAAGTCCCTCACCC
  TTGTCTTTGAGTACCTGGACAAGGACCTGAAGCAGTACCTGGATG
  
• Restriction Sites: Please inquire
• ACCN: NM_033018
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033018.2, NP_148978.1
• RefSeq Size: 3280 bp
• RefSeq ORF: 1491 bp
• Locus ID: 5127
• Cytogenetics: Xp11.3
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: 'The protein encoded by this gene belongs to the cdc2/cdkx subfamily of the ser/thr family of protein kinases. It may play a role in signal transduction cascades in terminally differentiated cells; in exocytosis; and in transport of secretory cargo from the endoplasmic reticulum. This gene is thought to escape X inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2009]'
  Transcript Variant: This variant (2) uses an alternate exon in the 5' UTR and 5' coding region, compared to variant 1. It encodes isoform 2, which has a longer and distinct N-terminus, compared to isoform 1.