PCTAIRE1 (CDK16) (NM_033018) Human Untagged Clone

CAT#: SC323582

CDK16 (untagged)-Kinase deficient mutant (K194M) of Human PCTAIRE protein kinase 1 (PCTK1), transcript variant 2


  "NM_033018" in other vectors (8)

Reconstitution Protocol

USD 660.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol CDK16
Synonyms PCTAIRE; PCTAIRE1; PCTGAIRE; PCTK1
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_033018, the custom clone sequence may differ by one or more nucleotides


ATGCAGTCCGAGATCGCCATGGATCGGATGAAGAAGATCAAACGGCAGCTGTCAATGACACTCCGAGGTG
GCCGAGGCATAGACAAGACCAATGGTGCCCCTGAGCAGATAGGCCTGGATGAGAGTGGTGGTGGTGGCGG
CAGTGACCCTGGAGAGGCCCCCACACGTGCTGCTCCTGGGGAACTTCGTTCTGCACGGGGCCCACTCAGC
TCTGCACCAGAGATTGTGCACGAGGACTTGAAGATGGGGTCTGATGGGGAGAGTGACCAGGCTTCAGCCA
CGTCCTCGGATGAGGTGCAGTCTCCAGTGAGAGTGCGTATGCGCAACCATCCCCCACGCAAGATCTCCAC
TGAGGACATCAACAAGCGCCTATCACTACCAGCTGACATCCGGCTGCCTGAGGGCTACCTGGAGAAGCTG
ACCCTCAATAGCCCCATCTTTGACAAGCCCCTCAGCCGCCGCCTCCGTCGTGTCAGCCTATCTGAGATTG
GCTTTGGGAAACTGGAGACCTACATTAAGCTGGACAAACTGGGCGAGGGTACCTATGCCACCGTCTACAA
AGGCAAAAGCAAGCTCACAGACAACCTTGTGGCACTCAAGGAGATCAGACTGGAACATGAAGAGGGGGCA
CCCTGCACCGCCATCCGGGAAGTGTCCCTGCTCAAGGACCTCAAACACGCCAACATCGTTACGCTACATG
ACATTATCCACACGGAGAAGTCCCTCACCCTTGTCTTTGAGTACCTGGACAAGGACCTGAAGCAGTACCT
GGATGACTGTGGGAACATCATCAACATGCACAACGTGAAACTGTTCCTGTTCCAGCTGCTCCGTGGCCTG
GCCTACTGCCACCGGCAGAAGGTGCTACACCGAGACCTCAAGCCCCAGAACCTGCTCATCAACGAGAGGG
GAGAGCTCAAGCTGGCTGACTTTGGCCTGGCCCGAGCCAAGTCAATCCCAACAAAGACATACTCCAATGA
GGTGGTGACACTGTGGTACCGGCCCCCTGACATCCTGCTTGGGTCCACGGACTACTCCACTCAGATTGAC
ATGTGGGGTGTGGGCTGCATCTTCTATGAGATGGCCACAGGCCGTCCCCTCTTTCCGGGCTCCACGGTGG
AGGAACAGCTACACTTCATCTTCCGTATCTTAGGAACCCCAACTGAGGAGACGTGGCCAGGCATCCTGTC
CAACGAGGAGTTCAAGACATACAACTACCCCAAGTACCGAGCCGAGGCCCTTTTGAGCCACGCACCCCGA
CTTGATAGCGACGGGGCCGACCTCCTCACCAAGCTGTTGCAGTTTGAGGGTCGAAATCGGATCTCCGCAG
AGGATGCCATGAAACATCCATTCTTCCTCAGTCTGGGGGAGCGGATCCACAAACTTCCTGACACTACTTC
CATATTTGCACTAAAGGAGATTCAGCTACAAAAGGAGGCCAGCCTTCGGTCTTCGTCGATGCCTGACTCA
GGCAGGCCAGCTTTCCGCGTGGTGGACACCGAGTTCTAA


>OriGene 5' read for mutant NM_033018 unedited
ACCGCCCGTACAGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTTCTATATAAGCAGAGCTCGTTTAGTT
GAACCGTCAGAATCTTGTAATACGACTCACTATAGGGCGGCCGCGAATACGGCACGAGCCTCCCCAAGCA
CCTCAAGGGACTAGAACTGAGCGCTTCATTTGTCTTTTTTCCTCCTTGCAAAAGTCCCGTATGCCACCAT
GGGGATGTACCAAGTGAGACCGAGTAGGGGGAACGAGTGGTGATTGACGCGCCAGGTTACTGGCCACTGC
TCACCTAGGCGCTAGCAAACTTTCTGCCAAGATCGGGAACTGAGTACTAAACAGCCCTCCACAGTTTCTC
CCTGGTGCCGTCTCCGGCTTGGCGCCGCATCCTCCCTCTGGGCTTCGCGATTGCCCGCGTCCCCCTCCCC
GCTGCGCGGAACGGGTTCCTTTTGTTCACATGCGCAGTCCGAGAAATCCGCCTATGATATCCGAAATGAA
AAAGATATCCAAACGGCAAGCTGTCATTGACCCTCCCCAGAGGTGGGCCAGAGGCTAAAAACAAGAACCA
ATGGTCCCCTGGACCAAATAGGCCTGATTAAAATTGGTGGGGGGGTGGGCGAATGTCACCCGGGGAAGAG
CCCCCCACCTGTGTGGGCCCGGGGGGAAATTTGTTTGCGCCGGGGGCCCCTCTCCTCTGTACCCAAAAAT
TTGCCCCAAGAAGTTTTGAATAATGTCTTCTAATGAAAAGATAAACCCTCTTACCCGTGTCTGAGAAGAG
GTCGATCCCTGAGAGAGCTTATGCGCCCCATCTCCCGCCGATTCTCCTGAGGGATATTAACGCCCATATA
CTCACTGCATCTCTGGCTCGAGAGCTCGTGAAAGACTCACTATACCCTCTTGATGAACCCCCTGCGCCCG
TCCGTTGGCACATTTAGATAGCTGTGAGAGCGACACTCAATTAGCAACAGCGAGGAGGATTACGGCTTCA
AGAGAGGAGTCACT
>SC323582 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
TSTGGCAGCTGGGCGAGGGTACCTATGCCRCCGTCKACAAAGGCRAAAGCAAGCTCACAGACAACCTTGT
GGCACTCATGGAGATCAGACTGGAACATGAAGAGGGGGCACCCTGCACCGCCATCCGGGAAGTGTCCCTG
CTCAAGGACCTCAAACACGCCAACATCGTTACGCTACATGACATTATCCACACGGAGAAGTCCCTCACCC
TTGTCTTTGAGTACCTGGACAAGGACCTGAAGCAGTACCTGGATG
Restriction Sites Please inquire     
ACCN NM_033018
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_033018.2, NP_148978.1
RefSeq Size 3280 bp
RefSeq ORF 1491 bp
Locus ID 5127
Cytogenetics Xp11.3
Domains pkinase, TyrKc, S_TKc
Protein Families Druggable Genome, Protein Kinase
Gene Summary 'The protein encoded by this gene belongs to the cdc2/cdkx subfamily of the ser/thr family of protein kinases. It may play a role in signal transduction cascades in terminally differentiated cells; in exocytosis; and in transport of secretory cargo from the endoplasmic reticulum. This gene is thought to escape X inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2009]'
Transcript Variant: This variant (2) uses an alternate exon in the 5' UTR and 5' coding region, compared to variant 1. It encodes isoform 2, which has a longer and distinct N-terminus, compared to isoform 1.

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