FAM151A (NM_176782) Human Tagged ORF Clone

CAT#: RC205057

FAM151A (Myc-DDK-tagged)-Human family with sequence similarity 151, member A (FAM151A)

Product Images

Western blot validation of overexpression lysate (Cat# LY406181) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205057 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified FAM151A protein (Cat# TP305057). The protein was produced from HEK293T cells transfected with FAM151A cDNA clone (Cat# RC205057) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FAM151A
• Synonyms: C1orf179
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205057 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCTGCAGGGAGCAGTTATCAAAGAATCAGGTCAAGTGGGTGTTTGCCGGCATTACCTGTGTGTCTG
  TGGTGGTCATTGCCGCAATAGTCCTTGCCATCACCCTGCGGCGGCCAGGCTGTGAGCTGGAGGCCTGCAG
  CCCTGATGCCGACATGCTGGACTACCTGCTGAGCCTGGGCCAGATCAGCCGGCGAGATGCCTTGGAGGTC
  ACCTGGTACCACGCAGCCAACAGCAAGAAAGCCATGACAGCTGCCCTGGACAGCAACATCACAGTCCTGG
  AGGCTGACGTCAATGTAGAAGGGCTCGGCACAGCCAATGAGACAGGAGTTCCCATCATGGCACACCCCCC
  CACTACCTACAGTGACAACACACTGGAGCAGTGGCTGGACGCTGTGCTGGGCTCTTCCCAAAAGGGCATC
  AAACTGGACTTCAAGAACATCAAGGCAGTGGGCCCCTCCCTGGACCTCCTGCGGCAGCTGACAGAGGAAG
  GCAAAGTCCGGCGGCCCATATGGATCAACGCTGACATCTTAAAGGGCCCCAACATGCTCATCTCAACTGA
  GGTCAATGCCACACAGTTCCTGGCCCTGGTCCAGGAGAAGTATCCCAAGGCTACCCTATCTCCAGGCTGG
  ACCACCTTCTACATGTCCACGTCCCCAAACAGGACGTACACCCAAGCCATGGTGGAGAAGATGCACGAGC
  TGGTGGGAGGAGTGCCCCAGAGGGTCACCTTCCCTGTACGGTCTTCCATGGTGCGGGCTGCCTGGCCCCA
  CTTCAGCTGGCTGCTGAGCCAATCTGAGAGGTACAGCCTGACGCTGTGGCAGGCTGCCTCGGACCCCATG
  TCGGTGGAAGATCTGCTCTACGTCCGGGATAACACTGCTGTCCACCAAGTCTACTATGACATCTTTGAGC
  CTCTCCTGTCACAGTTCAAGCAGCTGGCCTTGAATGCCACACGGAAACCAATGTACTACACGGGAGGCAG
  CCTGATCCCTCTTCTCCAGCTGCCTGGGGATGACGGTCTGAATGTGGAGTGGCTGGTTCCTGACGTCCAG
  GGCAGCGGTAAAACAGCAACAATGACCCTCCCAGACACAGAAGGCATGATCCTGCTGAACACTGGCCTCG
  AGGGAACTGTGGCTGAAAACCCCGTGCCCATTGTTCATACTCCAAGTGGCAACATCCTGACGCTGGAGTC
  CTGCCTGCAGCAGCTGGCCACACATCCCGGACACTGGGGCATCCATTTGCAAATAGTGGAGCCCGCAGCC
  CTCCGGCCATCCCTGGCCTTGCTGGCACGCCTCTCCAGCCTTGGCCTCTTGCATTGGCCTGTGTGGGTTG
  GGGCCAAAATCTCCCACGGGAGTTTTTCGGTCCCCGGCCATGTGGCTGGCAGAGAGCTGCTTACAGCTGT
  GGCTGAGGTCTTCCCCCACGTGACTGTGGCACCAGGCTGGCCTGAGGAGGTGCTGGGCAGTGGCTACAGG
  GAACAGCTGCTCACAGATATGCTAGAGTTGTGCCAGGGGCTCTGGCAACCTGTGTCCTTCCAGATGCAGG
  CCATGCTGCTGGGCCACAGCACAGCTGGAGCCATAGGCAGGCTGCTGGCATCCTCCCCCCGGGCCACCGT
  CACAGTGGAGCACAACCCAGCTGGGGGCGACTATGCCTCTGTGAGGACAGCATTGCTGGCAGCTAGGGCT
  GTGGACAGGACCCGAGTCTACTACAGGCTACCCCAGGGCTACCACAAGGACTTGCTGGCTCATGTTGGTA
  GAAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205057 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVCREQLSKNQVKWVFAGITCVSVVVIAAIVLAITLRRPGCELEACSPDADMLDYLLSLGQISRRDALEV
  TWYHAANSKKAMTAALDSNITVLEADVNVEGLGTANETGVPIMAHPPTTYSDNTLEQWLDAVLGSSQKGI
  KLDFKNIKAVGPSLDLLRQLTEEGKVRRPIWINADILKGPNMLISTEVNATQFLALVQEKYPKATLSPGW
  TTFYMSTSPNRTYTQAMVEKMHELVGGVPQRVTFPVRSSMVRAAWPHFSWLLSQSERYSLTLWQAASDPM
  SVEDLLYVRDNTAVHQVYYDIFEPLLSQFKQLALNATRKPMYYTGGSLIPLLQLPGDDGLNVEWLVPDVQ
  GSGKTATMTLPDTEGMILLNTGLEGTVAENPVPIVHTPSGNILTLESCLQQLATHPGHWGIHLQIVEPAA
  LRPSLALLARLSSLGLLHWPVWVGAKISHGSFSVPGHVAGRELLTAVAEVFPHVTVAPGWPEEVLGSGYR
  EQLLTDMLELCQGLWQPVSFQMQAMLLGHSTAGAIGRLLASSPRATVTVEHNPAGGDYASVRTALLAARA
  VDRTRVYYRLPQGYHKDLLAHVGRN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_176782
• ORF Size: 1755 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_176782.1, NM_176782.2, NP_788954.1
• RefSeq Size: 1998 bp
• RefSeq ORF: 1758 bp
• Locus ID: 338094
• Protein Families: Transmembrane
• MW: 64 kDa