AASDHPPT (NM_015423) Human Tagged ORF Clone

CAT#: RC205091

AASDHPPT (Myc-DDK-tagged)-Human aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase (AASDHPPT)

Product Images

Western blot validation of overexpression lysate (Cat# LY414583) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205091 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified AASDHPPT protein (Cat# TP305091). The protein was produced from HEK293T cells transfected with AASDHPPT cDNA clone (Cat# RC205091) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: AASDHPPT
• Synonyms: AASD-PPT; ACPS; CGI-80; LYS2; LYS5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205091 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTTTCCCTGCCAAACGGTTCTGCTTGGTGCCATCCATGGAGGGCGTGCGCTGGGCCTTTTCCTGCG
  GCACTTGGCTGCCGAGCCGAGCCGAATGGCTGCTGGCAGTGCGATCGATTCAGCCCGAGGAGAAGGAGCG
  CATTGGCCAGTTCGTCTTTGCCCGGGACGCTAAGGCAGCCATGGCTGGTCGTCTGATGATAAGGAAATTA
  GTTGCAGAGAAATTGAATATCCCTTGGAATCATATTCGTTTGCAAAGAACTGCAAAAGGAAAACCAGTTC
  TTGCAAAGGACTCATCGAATCCTTACCCGAATTTCAACTTTAACATCTCTCATCAAGGAGACTATGCAGT
  GCTTGCTGCTGAACCTGAGCTGCAAGTTGGAATTGATATAATGAAGACTAGTTTTCCAGGTCGTGGTTCA
  ATTCCAGAATTCTTTCATATTATGAAAAGAAAGTTTACCAACAAAGAATGGGAAACAATCAGAAGCTTTA
  AGGATGAGTGGACTCAGCTGGATATGTTTTATAGGAATTGGGCACTTAAGGAAAGCTTCATAAAAGCCAT
  TGGTGTTGGACTAGGATTTGAATTGCAGCGGCTTGAATTTGATCTATCTCCATTAAACTTGGATATAGGC
  CAAGTTTATAAAGAAACACGTTTATTCCTGGATGGAGAGGAAGAAAAAGAATGGGCATTTGAGGAAAGCA
  AAATAGATGAGCACCATTTTGTTGCAGTTGCTCTTAGGAAACCCGATGGATCTAGACATCAGGATGTTCC
  ATCTCAGGATGATTCCAAACCAACCCAGAGGCAATTTACTATTCTCAACTTTAATGATTTAATGTCATCT
  GCCGTTCCCATGACACCTGAAGATCCTTCATTTTGGGACTGTTTTTGCTTCACAGAAGAAATTCCAATAC
  GAAATGGTACAAAGTCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205091 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVFPAKRFCLVPSMEGVRWAFSCGTWLPSRAEWLLAVRSIQPEEKERIGQFVFARDAKAAMAGRLMIRKL
  VAEKLNIPWNHIRLQRTAKGKPVLAKDSSNPYPNFNFNISHQGDYAVLAAEPELQVGIDIMKTSFPGRGS
  IPEFFHIMKRKFTNKEWETIRSFKDEWTQLDMFYRNWALKESFIKAIGVGLGFELQRLEFDLSPLNLDIG
  QVYKETRLFLDGEEEKEWAFEESKIDEHHFVAVALRKPDGSRHQDVPSQDDSKPTQRQFTILNFNDLMSS
  AVPMTPEDPSFWDCFCFTEEIPIRNGTKS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015423
• ORF Size: 927 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015423.1, NM_015423.2, NP_056238.2
• RefSeq Size: 2880 bp
• RefSeq ORF: 930 bp
• Locus ID: 60496
• Domains: ACPS
• Protein Pathways: Lysine biosynthesis, Lysine degradation, Metabolic pathways
• MW: 35.8 kDa
• Gene Summary: The protein encoded by this gene is similar to Saccharomyces cerevisiae LYS5, which is required for the activation of the alpha-aminoadipate dehydrogenase in the biosynthetic pathway of lysine. Yeast alpha-aminoadipate dehydrogenase converts alpha-biosynthetic-aminoadipate semialdehyde to alpha-aminoadipate. It has been suggested that defects in the human gene result in pipecolic acidemia. [provided by RefSeq, Jul 2008]