AASDHPPT (NM_015423) Human Tagged ORF Clone

CAT#: RG205091

AASDHPPT (GFP-tagged) - Human aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase (AASDHPPT)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: AASDHPPT
• Synonyms: AASD-PPT; ACPS; CGI-80; LYS2; LYS5
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG205091 representing NM_015423
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTTTCCCTGCCAAACGGTTCTGCTTGGTGCCATCCATGGAGGGCGTGCGCTGGGCCTTTTCCTGCG
  GCACTTGGCTGCCGAGCCGAGCCGAATGGCTGCTGGCAGTGCGATCGATTCAGCCCGAGGAGAAGGAGCG
  CATTGGCCAGTTCGTCTTTGCCCGGGACGCTAAGGCAGCCATGGCTGGTCGTCTGATGATAAGGAAATTA
  GTTGCAGAGAAATTGAATATCCCTTGGAATCATATTCGTTTGCAAAGAACTGCAAAAGGAAAACCAGTTC
  TTGCAAAGGACTCATCGAATCCTTACCCGAATTTCAACTTTAACATCTCTCATCAAGGAGACTATGCAGT
  GCTTGCTGCTGAACCTGAGCTGCAAGTTGGAATTGATATAATGAAGACTAGTTTTCCAGGTCGTGGTTCA
  ATTCCAGAATTCTTTCATATTATGAAAAGAAAGTTTACCAACAAAGAATGGGAAACAATCAGAAGCTTTA
  AGGATGAGTGGACTCAGCTGGATATGTTTTATAGGAATTGGGCACTTAAGGAAAGCTTCATAAAAGCCAT
  TGGTGTTGGACTAGGATTTGAATTGCAGCGGCTTGAATTTGATCTATCTCCATTAAACTTGGATATAGGC
  CAAGTTTATAAAGAAACACGTTTATTCCTGGATGGAGAGGAAGAAAAAGAATGGGCATTTGAGGAAAGCA
  AAATAGATGAGCACCATTTTGTTGCAGTTGCTCTTAGGAAACCCGATGGATCTAGACATCAGGATGTTCC
  ATCTCAGGATGATTCCAAACCAACCCAGAGGCAATTTACTATTCTCAACTTTAATGATTTAATGTCATCT
  GCCGTTCCCATGACACCTGAAGATCCTTCATTTTGGGACTGTTTTTGCTTCACAGAAGAAATTCCAATAC
  GAAATGGTACAAAGTCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG205091 representing NM_015423
  Red=Cloning site Green=Tags(s)
  
  MVFPAKRFCLVPSMEGVRWAFSCGTWLPSRAEWLLAVRSIQPEEKERIGQFVFARDAKAAMAGRLMIRKL
  VAEKLNIPWNHIRLQRTAKGKPVLAKDSSNPYPNFNFNISHQGDYAVLAAEPELQVGIDIMKTSFPGRGS
  IPEFFHIMKRKFTNKEWETIRSFKDEWTQLDMFYRNWALKESFIKAIGVGLGFELQRLEFDLSPLNLDIG
  QVYKETRLFLDGEEEKEWAFEESKIDEHHFVAVALRKPDGSRHQDVPSQDDSKPTQRQFTILNFNDLMSS
  AVPMTPEDPSFWDCFCFTEEIPIRNGTKS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015423
• ORF Size: 927 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_015423.2, NP_056238.2
• RefSeq Size: 2880
• RefSeq ORF: 930
• Locus ID: 60496
• Domains: ACPS
• Protein Pathways: Lysine biosynthesis, Lysine degradation, Metabolic pathways
• Gene Summary: The protein encoded by this gene is similar to Saccharomyces cerevisiae LYS5, which is required for the activation of the alpha-aminoadipate dehydrogenase in the biosynthetic pathway of lysine. Yeast alpha-aminoadipate dehydrogenase converts alpha-biosynthetic-aminoadipate semialdehyde to alpha-aminoadipate. It has been suggested that defects in the human gene result in pipecolic acidemia. [provided by RefSeq, Jul 2008]