RPL6 (NM_001024662) Human Tagged ORF Clone

CAT#: RC205119

RPL6 (Myc-DDK-tagged)-Human ribosomal protein L6 (RPL6), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY422528) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205119 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RPL6
• Synonyms: L6; SHUJUN-2; TAXREB107; TXREB1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205119 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGTGAAAAAGTTGAGAAGCCAGATACTAAAGAGAAGAGACCCGAAGCCAAGAAGGTTGATGCTG
  GTGGCAAGGTGAAAAAGGGTAACCTCAAAGCTAAAAAGCCCAAGAAGGGGAAGCCCCATTGCAGCCGCAA
  CCCTGTCCTTGTCAGAGGAGTTGGCAGGTATTCCCGATCTGCCATGTATTCCAGAAAGGCCATGTACAAG
  AGGAAGTACTCAGCCGCTAAATCCAAGGTTGAAAAGAAAAAGAAGGAGAAGGTTCTCGCAACTGTTACAA
  AACCAGTTGGTGGTGACAAGAACGGCGGTACCCGGGTGGTTAAACTTCGCAAAATGCCTAGATATTATCC
  TACTGAAGATGTGCCTCGAAAGCTGTTGAGCCACGGCAAAAAACCCTTCAGTCAGCACGTGAGAAAACTG
  CGAGCCAGCATTACCCCCGGGACCATTCTGATCATCCTCACTGGACGCCACAGGGGCAAGAGGGTGGTTT
  TCCTGAAGCAGCTGGCTAGTGGCTTATTACTTGTGACTGGACCTCTGGTCCTCAATCGAGTTCCTCTACG
  AAGAACACACCAGAAATTTGTCATTGCCACTTCAACCAAAATCGATATCAGCAATGTAAAAATCCCAAAA
  CATCTTACTGATGCTTACTTCAAGAAGAAGAAGCTGCGGAAGCCCAGACACCAGGAAGGTGAGATCTTCG
  ACACAGAAAAAGAGAAATATGAGATTACGGAGCAGCGCAAGATTGATCAGAAAGCTGTGGACTCACAAAT
  TTTACCAAAAATCAAAGCTATTCCTCAGCTCCAGGGCTACCTGCGATCTGTGTTTGCTCTGACGAATGGA
  ATTTATCCTCACAAATTGGTGTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205119 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAGEKVEKPDTKEKRPEAKKVDAGGKVKKGNLKAKKPKKGKPHCSRNPVLVRGVGRYSRSAMYSRKAMYK
  RKYSAAKSKVEKKKKEKVLATVTKPVGGDKNGGTRVVKLRKMPRYYPTEDVPRKLLSHGKKPFSQHVRKL
  RASITPGTILIILTGRHRGKRVVFLKQLASGLLLVTGPLVLNRVPLRRTHQKFVIATSTKIDISNVKIPK
  HLTDAYFKKKKLRKPRHQEGEIFDTEKEKYEITEQRKIDQKAVDSQILPKIKAIPQLQGYLRSVFALTNG
  IYPHKLVF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001024662
• ORF Size: 864 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001024662.1, NM_001024662.2, NP_001019833.1
• RefSeq Size: 1148 bp
• RefSeq ORF: 867 bp
• Locus ID: 6128
• Cytogenetics: 12q24.13
• Protein Families: Transcription Factors
• Protein Pathways: Ribosome
• MW: 32.7 kDa
• Gene Summary: 'This gene encodes a protein component of the 60S ribosomal subunit. This protein can bind specifically to domain C of the tax-responsive enhancer element of human T-cell leukemia virus type 1, and may participate in tax-mediated transactivation of transcription. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed throughout the genome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]'