Cbl c (CBLC) (NM_012116) Human Tagged ORF Clone

CAT#: RC205130

CBLC (Myc-DDK-tagged)-Human Cas-Br-M (murine) ecotropic retroviral transforming sequence c (CBLC), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY402148) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205130 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CBLC protein (Cat# TP305130). The protein was produced from HEK293T cells transfected with CBLC cDNA clone (Cat# RC205130) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CBLC
• Synonyms: CBL-3; CBL-SL; RNF57
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205130 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTGGCGGTGGCCCCGTGGGGGCGACAGTGGGAAGAGGCCCGCGCCCTGGGCCGGGCAGTCAGGA
  TGCTGCAGCGCCTAGAAGAGCAATGCGTCGACCCCCGGCTGTCCGTGAGTCCCCCTTCGCTGCGGGACCT
  GCTGCCCCGCACAGCGCAGCTGCTTCGAGAGGTGGCCCATTCTCGGCGGGCGGCCGGCGGAGGCGGCCCC
  GGGGGTCCCGGCGGCTCTGGGGACTTTCTACTCATCTACCTGGCCAATCTGGAGGCCAAGAGCAGGCAGG
  TGGCCGCGCTGCTGCCTCCCCGGGGCCGAAGGAGTGCCAACGACGAGCTCTTCCGGGCGGGCTCCAGACT
  CAGGCGACAGCTGGCCAAGCTGGCCATCATCTTCAGCCACATGCACGCAGAGCTGCACGCACTCTTCCCC
  GGGGGAAAGTACTGTGGACACATGTACCAGCTCACCAAGGCCCCCGCCCACACCTTCTGGAGGGAAAGTT
  GCGGAGCCCGGTGTGTGCTGCCCTGGGCTGAGTTTGAGTCCCTCCTGGGCACCTGCCACCCTGTGGAACC
  AGGCTGCACAGCCCTGGCCTTGCGCACCACCATTGACCTCACCTGCAGCGGGCACGTGTCCATCTTCGAG
  TTCGACGTCTTCACCAGGCTCTTTCAGCCATGGCCAACACTCCTCAAGAACTGGCAGCTCCTGGCAGTCA
  ACCACCCAGGCTACATGGCCTTCCTCACCTATGATGAGGTCCAAGAGCGTCTGCAGGCCTGCAGGGACAA
  GCCAGGCAGTTACATCTTCCGGCCCAGCTGTACTCGCCTGGGGCAGTGGGCCATCGGCTATGTGAGCTCA
  GATGGCAGCATCCTGCAGACCATCCCTGCCAACAAACCCCTGTCCCAGGTGCTCCTGGAGGGACAGAAGG
  ACGGCTTCTACCTCTACCCAGATGGAAAGACCCACAACCCAGACCTGACTGAGCTCGGCCAGGCAGAACC
  CCAGCAGCGCATCCACGTGTCAGAGGAGCAGCTGCAGCTCTACTGGGCCATGGACTCCACATTTGAGCTC
  TGCAAGATCTGTGCTGAGAGCAACAAGGATGTGAAGATTGAGCCGTGCGGGCACCTGCTCTGCAGCTGCT
  GCCTGGCTGCCTGGCAGCACTCGGACAGCCAGACCTGCCCCTTCTGCCGCTGCGAGATCAAGGGCTGGGA
  GGCCGTGAGTATCTACCAGTTCCACGGTCAGGCTACTGCTGAGGACCCAGGGAACAGCAGTGACCAGGAA
  GGCAGGGAGTTGGAGCTGGGGCAGGTGCCCCTTTCGGCTCCTCCATTGCCCCCACGGCCAGATCTGCCCC
  CCAGGAAGCCCAGAAATGCCCAGCCGAAAGTGAGACTCCTAAAGGGGAACTCCCCTCCAGCTGCGCTGGG
  ACCCCAGGACCCTGCCCCGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205130 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MALAVAPWGRQWEEARALGRAVRMLQRLEEQCVDPRLSVSPPSLRDLLPRTAQLLREVAHSRRAAGGGGP
  GGPGGSGDFLLIYLANLEAKSRQVAALLPPRGRRSANDELFRAGSRLRRQLAKLAIIFSHMHAELHALFP
  GGKYCGHMYQLTKAPAHTFWRESCGARCVLPWAEFESLLGTCHPVEPGCTALALRTTIDLTCSGHVSIFE
  FDVFTRLFQPWPTLLKNWQLLAVNHPGYMAFLTYDEVQERLQACRDKPGSYIFRPSCTRLGQWAIGYVSS
  DGSILQTIPANKPLSQVLLEGQKDGFYLYPDGKTHNPDLTELGQAEPQQRIHVSEEQLQLYWAMDSTFEL
  CKICAESNKDVKIEPCGHLLCSCCLAAWQHSDSQTCPFCRCEIKGWEAVSIYQFHGQATAEDPGNSSDQE
  GRELELGQVPLSAPPLPPRPDLPPRKPRNAQPKVRLLKGNSPPAALGPQDPAPA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012116
• ORF Size: 1422 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012116.2, NM_012116.3, NP_036248.2
• RefSeq Size: 1591 bp
• RefSeq ORF: 1425 bp
• Locus ID: 23624
• Protein Families: Druggable Genome
• Protein Pathways: Chronic myeloid leukemia, Endocytosis, ErbB signaling pathway, Insulin signaling pathway, Jak-STAT signaling pathway, Pathways in cancer, T cell receptor signaling pathway, Ubiquitin mediated proteolysis
• MW: 52.5 kDa
• Gene Summary: This gene encodes a member of the Cbl family of E3 ubiquitin ligases. Cbl proteins play important roles in cell signaling through the ubiquitination and subsequent downregulation of tyrosine kinases. Expression of this gene may be restricted to epithelial cells, and alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012]