FGD1 (NM_004463) Human Tagged ORF Clone

CAT#: RC205133

FGD1 (Myc-DDK-tagged)-Human FYVE, RhoGEF and PH domain containing 1 (FGD1)

Product Images

Western blot validation of overexpression lysate (Cat# LY417971) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205133 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified FGD1 protein (Cat# TP305133). The protein was produced from HEK293T cells transfected with FGD1 cDNA clone (Cat# RC205133) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FGD1
• Synonyms: AAS; FGDY; MRXS16; ZFYVE3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205133 representing NM_004463
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATGGCCACCGAGCCCCGGGGGGCGCCGGGCCTTCGGAGCCCGAACACCCGGCCACGAACCCGCCGG
  GCGCCGCTCCGCCGGCCTGTGCCGACTCGGACCCTGGAGCCTCGGAACCCGGACTGCTGGCGCGCAGGGG
  CTCAGGTTCGGCTCTTGGCGGCCCACTGGATCCCCAGTTTGTCGGACCCTCGGACACCAGCCTGGGCGCT
  GCTCCAGGCCACCGGGTCTTGCCCTGCGGTCCCAGTCCACAGCACCACCGGGCCCTGCGCTTCTCTTACC
  ACCTGGAGGGCTCGCAGCCTCGGCCTGGGCTGCACCAGGGAAACCGGATCCTGGTTAAAAGTTTGTCCCT
  TGACCCTGGCCAAAGCCTAGAGCCTCATCCAGAAGGTCCCCAGCGGCTTCGCTCAGACCCAGGTCCCCCG
  ACTGAAACCCCTAGCCAGCGTCCTTCACCACTGAAGCGGGCACCGGGCCCGAAGCCACAGGTGCCCCCAA
  AGCCCAGCTACCTGCAGATGCCCCGGATGCCCCCCCCACTGGAGCCCATCCCCCCTCCACCATCACGCCC
  ACTGCCTGCCGACCCCCGAGTGGCCAAGGGCCTGGCTCCCAGGGCAGAGGCCAGCCCCAGTTCTGCAGCA
  GTATCCTCACTGATTGAGAAGTTTGAAAGAGAGCCTGTGATTGTCGCCTCGGATAGACCAGTCCCTGGCC
  CCAGCCCAGGTCCCCCAGAGCCAGTCATGTTGCCACAGCCAACCTCGCAGCCACCAGTGCCCCAGCTCCC
  CGAGGGTGAGGCCTCCCGCTGCCTGTTTCTGCTGGCTCCTGGGCCCCGGGACGGTGAGAAGGTGCCCAAC
  CGGGACAGCGGCATTGATAGCATCAGCTCGCCATCCAACAGCGAGGAGACCTGCTTCGTCAGTGATGACG
  GGCCCCCCAGCCACAGCCTCTGCCCTGGGCCCCCTGCCCTGGCTAGTGTGCCTGTTGCCTTGGCCGACCC
  CCACCGGCCTGGCTCCCAAGAGGTTGACAGTGACCTGGAGGAGGAGGACGACGAGGAGGAGGAGGAAGAG
  AAGGACAGAGAAATCCCAGTGCCCCTGATGGAGAGACAGGAGTCTGTGGAGTTGACTGTGCAGCAAAAGG
  TGTTTCACATTGCCAATGAGCTCCTGCAAACTGAGAAGGCCTACGTTTCCAGGCTCCATCTCCTGGATCA
  GGTGTTCTGTGCCCGGCTGCTGGAAGAAGCTCGGAACCGCAGTTCCTTCCCGGCCGACGTTGTCCACGGC
  ATCTTCTCTAACATCTGCTCCATCTATTGCTTCCACCAGCAGTTCCTGCTGCCTGAGCTAGAGAAGCGCA
  TGGAGGAATGGGACCGCTATCCACGCATTGGAGACATCCTGCAGAAACTGGCCCCCTTCCTCAAGATGTA
  TGGTGAGTATGTGAAGAACTTTGACCGGGCCGTGGAGCTGGTCAACACCTGGACAGAGCGCTCCACCCAG
  TTTAAAGTCATCATCCATGAGGTGCAGAAGGAGGAAGCCTGTGGCAACCTGACATTGCAGCACCACATGC
  TGGAGCCTGTGCAGCGCATCCCCCGCTATGAGCTTCTTCTCAAGGACTATCTGTTAAAGCTGCCCCATGG
  CTCCCCGGACAGCAAGGATGCCCAAAAGTCTCTGGAGCTGATCGCCACAGCAGCAGAGCACTCGAATGCT
  GCCATCCGCAAAATGGAGCGAATGCATAAGCTGCTGAAGGTATATGAGCTGTTAGGGGGCGAGGAGGACA
  TTGTCAGCCCCACCAAAGAGCTCATAAAAGAAGGCCACATCCTTAAGCTGTCAGCAAAGAATGGGACCAC
  TCAAGACCGATACCTCATACTATTCAACGACCGCCTCCTTTACTGCGTGCCCAGGCTGCGGCTCCTTGGC
  CAGAAGTTTAGCGTGCGGGCACGCATTGATGTAGATGGCATGGAGCTAAAGGAGAGCTCCAACCTCAATC
  TGCCTCGAACCTTCCTGGTGTCAGGAAAGCAGCGCTCCCTCGAGCTCCAGGCCAGGACTGAGGAGGAGAA
  GAAAGACTGGGTCCAGGCCATCAACTCCACCCTCCTGAAGCATGAACAGACGCTGGAGACTTTCAAACTG
  TTGAACTCAACAAACAGGGAAGATGAAGACACCCCACCCAACTCTCCAAACGTGGATCTTGGGAAGCGGG
  CACCTACGCCCATCCGGGAAAAGGAAGTCACCATGTGCATGCGCTGCCAGGAGCCCTTCAATTCTATCAC
  CAAACGCAGGCACCACTGCAAGGCCTGCGGGCATGTGGTTTGTGGGAAGTGCTCCGAGTTCCGGGCCCGC
  CTCGTCTATGACAACAACCGCTCCAACCGTGTGTGCACTGATTGCTATGTGGCCTTGCACGGGGTGCCTG
  GGAGCAGTCCAGCCTGCAGCCAGCATACACCCCAGCGCCGGAGGTCCATCCTGGAGAAACAGGCCTCAGT
  GGCTGCAGAGAACAGCGTCATCTGCAGCTTCCTGCACTACATGGAGAAGGGTGGCAAAGGATGGCACAAG
  GCATGGTTCGTGGTCCCTGAAAATGAACCCTTGGTGCTGTATATCTACGGAGCCCCTCAGGATGTGAAAG
  CCCAGCGCAGCCTGCCCCTCATTGGCTTCGAGGTGGGACCGCCCGAGGCAGGGGAGCGGCCTGACAGAAG
  GCATGTCTTCAAGATCACCCAGAGCCACCTCAGCTGGTACTTCAGCCCTGAGACAGAGGAACTACAGCGA
  CGCTGGATGGCTGTGCTTGGCCGGGCGGGCCGAGGGGACACGTTCTGCCCGGGGCCCACACTGTCTGAGG
  ACAGGGAGATGGAGGAGGCACCGGTGGCTGCTTTAGGAGCCACTGCTGAACCCCCCGAATCCCCCCAGAC
  CCGAGACAAGACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205133 representing NM_004463
  Red=Cloning site Green=Tags(s)
  
  MHGHRAPGGAGPSEPEHPATNPPGAAPPACADSDPGASEPGLLARRGSGSALGGPLDPQFVGPSDTSLGA
  APGHRVLPCGPSPQHHRALRFSYHLEGSQPRPGLHQGNRILVKSLSLDPGQSLEPHPEGPQRLRSDPGPP
  TETPSQRPSPLKRAPGPKPQVPPKPSYLQMPRMPPPLEPIPPPPSRPLPADPRVAKGLAPRAEASPSSAA
  VSSLIEKFEREPVIVASDRPVPGPSPGPPEPVMLPQPTSQPPVPQLPEGEASRCLFLLAPGPRDGEKVPN
  RDSGIDSISSPSNSEETCFVSDDGPPSHSLCPGPPALASVPVALADPHRPGSQEVDSDLEEEDDEEEEEE
  KDREIPVPLMERQESVELTVQQKVFHIANELLQTEKAYVSRLHLLDQVFCARLLEEARNRSSFPADVVHG
  IFSNICSIYCFHQQFLLPELEKRMEEWDRYPRIGDILQKLAPFLKMYGEYVKNFDRAVELVNTWTERSTQ
  FKVIIHEVQKEEACGNLTLQHHMLEPVQRIPRYELLLKDYLLKLPHGSPDSKDAQKSLELIATAAEHSNA
  AIRKMERMHKLLKVYELLGGEEDIVSPTKELIKEGHILKLSAKNGTTQDRYLILFNDRLLYCVPRLRLLG
  QKFSVRARIDVDGMELKESSNLNLPRTFLVSGKQRSLELQARTEEEKKDWVQAINSTLLKHEQTLETFKL
  LNSTNREDEDTPPNSPNVDLGKRAPTPIREKEVTMCMRCQEPFNSITKRRHHCKACGHVVCGKCSEFRAR
  LVYDNNRSNRVCTDCYVALHGVPGSSPACSQHTPQRRRSILEKQASVAAENSVICSFLHYMEKGGKGWHK
  AWFVVPENEPLVLYIYGAPQDVKAQRSLPLIGFEVGPPEAGERPDRRHVFKITQSHLSWYFSPETEELQR
  RWMAVLGRAGRGDTFCPGPTLSEDREMEEAPVAALGATAEPPESPQTRDKT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004463
• ORF Size: 2883 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004463.1, NM_004463.2, NP_004454.2
• RefSeq Size: 4291 bp
• RefSeq ORF: 2886 bp
• Locus ID: 2245
• Cytogenetics: Xp11.22
• Protein Pathways: Regulation of actin cytoskeleton
• MW: 106.4 kDa
• Gene Summary: 'This gene encodes a protein that contains Dbl (DH) and pleckstrin (PH) homology domains and is similar to the Rho family of small GTP-binding proteins. The encoded protein specifically binds to the Rho family GTPase Cdc42Hs and can stimulate the GDP-GTP exchange of the isoprenylated form of Cdc42Hs. It also stimulates the mitogen activated protein kinase cascade leading to c-Jun kinase SAPK/JNK1 activation. Defects in this gene are the cause of the faciogenital dysplasia in Aarskog-Scott syndrome and a syndromatic form of X-linked cognitive disability. [provided by RefSeq, Jul 2017]'