PHYHIP (NM_014759) Human Tagged ORF Clone

CAT#: RC205205

PHYHIP (Myc-DDK-tagged)-Human phytanoyl-CoA 2-hydroxylase interacting protein (PHYHIP), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY420432) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220713 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PHYHIP protein (Cat# TP305205). The protein was produced from HEK293T cells transfected with PHYHIP cDNA clone (Cat# RC205205) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PHYHIP
• Synonyms: DYRK1AP3; PAHX-AP; PAHXAP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205205 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTGCTGTCCACGCCCCACAGCATTGAGATCAACAACATCACCTGCGACTCCTTCAGCATCTCCT
  GGGCCATGGAGGACAGTGACCTGGAGAGGGTCACCCATTACTTCATTGACCTTAACAAGAAGGAGAATAA
  GAATTCCAACAAGTTCAAGCACCGGGACGTCCCCACCAAGCTCGTGGCCAAGGCAGTGCCGCTGCCCATG
  ACGGTGAGAGGCCACTGGTTCCTGAGCCCCCGCACGGAGTACAGTGTGGCCGTGCAGACGGCAGTGAAGC
  AGAGCGATGGGGAGTACCTGGTGTCCGGCTGGAGCGAGACGGTGGAGTTCTGCACTGGGGATTATGCCAA
  GGAGCACCTGGCTCAGCTTCAGGAGAAAGCTGAGCAGATCGCAGGCCGCATGCTCCGCTTCTCCGTCTTC
  TACCGCAACCATCACAAGGAGTACTTCGAGCATGCCAGGACCCACTGCGGGAACATGCTGCAGCCTTACC
  TGAAGGACAACAGCGGCAGCCACGGCTCCCCCACCAGCGGTATGCTCCACGGGGTCTTCTTCAGCTGCAA
  CACGGAGTTCAACACGGGCCAGCCCCCGCAGGACTCCCCCTACGGCCGCTGGCGCTTCCAGATCCCAGCT
  CAGCGCCTCTTCAATCCCAGCACCAACCTCTACTTTGCGGACTTCTACTGCATGTACACGGCCTACCACT
  ACGCCATCCTGGTGCTGGCGCCCAAAGGCTCCATGGGGGACCGCTTCTGCCGCGACCGCCGGCCCCTCCT
  GGACATTGCTTGCAACAAGTTCCTGACCTGCAGCGTGGAGGATGGGGAGCTGGTCTTCCGCCACGCCCAG
  GACCTCATCCTGGAGATCATCTACACTGAGCCCGTCGACCTGTCCCTGGGCACCCTGGGGGAGATCAGTG
  GGCACCAGCTCATGAGTCTGTCTACTGCCGATGCCAAGAAGGACCCCAGCTGCAAGACCTGCAACATCAG
  CGTGGGCCGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205205 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MELLSTPHSIEINNITCDSFSISWAMEDSDLERVTHYFIDLNKKENKNSNKFKHRDVPTKLVAKAVPLPM
  TVRGHWFLSPRTEYSVAVQTAVKQSDGEYLVSGWSETVEFCTGDYAKEHLAQLQEKAEQIAGRMLRFSVF
  YRNHHKEYFEHARTHCGNMLQPYLKDNSGSHGSPTSGMLHGVFFSCNTEFNTGQPPQDSPYGRWRFQIPA
  QRLFNPSTNLYFADFYCMYTAYHYAILVLAPKGSMGDRFCRDRRPLLDIACNKFLTCSVEDGELVFRHAQ
  DLILEIIYTEPVDLSLGTLGEISGHQLMSLSTADAKKDPSCKTCNISVGR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014759
• ORF Size: 990 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014759.1, NM_014759.2, NM_014759.3, NP_055574.2
• RefSeq Size: 3232 bp
• RefSeq ORF: 993 bp
• Locus ID: 9796
• Domains: FN3
• MW: 37.6 kDa
• Gene Summary: Its interaction with PHYH suggests a role in the development of the central system. [UniProtKB/Swiss-Prot Function]