FNDC8 (NM_017559) Human Tagged ORF Clone

CAT#: RC205252

FNDC8 (Myc-DDK-tagged)-Human fibronectin type III domain containing 8 (FNDC8)

Product Images

Western blot validation of overexpression lysate (Cat# LY413703) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205252 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified FNDC8 protein (Cat# TP305252). The protein was produced from HEK293T cells transfected with FNDC8 cDNA clone (Cat# RC205252) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FNDC8
• Synonyms: DKFZp434H2215
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205252 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATCAGAGGCACTCCATCAAGTGGGAGATGGGGAGGAGGCTGTACTGAAGAAAGAAAACTTCAACA
  TGATGAATGCCCTTGACCAACTGCCAAAACCCTTTCCAAACCCCAAGTCTATGAACCGGACCGTCACTAC
  CAAAGGACTCCCACTAGCCTCAAAGGGCAATTTGGTCAACTTCTTGGAGGATGATACCATCAACCTACTG
  AAGCCGCTGCCAGTAGAGGATTCAGACTGCAGCTCTGATGAGACCAGCATCTCTGCCTTCTCATCCACCT
  TGCTGAACCCCATCAAATTAGCTGTGACCCAGCCCAACAGCAGCTTCTTTGCAGGGATGCTGGAGGGGGA
  GCTGAACAAACTCAGCTTCTCCCCAATGGCCAAGAATGCAGAAAATGAGGACCTGGCGCTCGGCCCCTGC
  CCATGCCCATCGAAGTCCCAGATGGCCACAAGGGGCCTGCTGGACCTTGACAACCCTGAGCTGGAGACAG
  AAACCTCCTCAACGCACTCAGAATCTTCTGTGGTTGTGGACCTGCCGGACACCCCCTTCATCTTTGAGCA
  CACAGTCAACAATTCCACAGCTGTGATTTCCTGGACCTACGCCTTGGGCAAGCAGCCGGTCAGTTTCTAC
  CAGCTCCTGTTACAGGAGGTGGCCAAGACACAGGAGAATGAGTTGCCCGAGGCAAAGAATCGTCCATGGA
  TCTTCAACAAGATTTTGGGCACTACTGTCAAGCTGATGGAGCTAAAGCCTAACACGTGTTACTGCCTCAG
  TGTCCGTGCAGCCAACACAGCTGGGGTGGGGAAGTGGTGCAAGCCCTACAAATTTGCAACCCTGGCCACT
  GACTTCAGCAGCTTTCCTGAGAACTACCCCATCCAGATCACCGTGCGGCGCAAGGAACCCCGGCAAAAGA
  TCGTGTCCATCGGGCCGGAGGAGATGCGGAGGCTGGAGGATCTGGAATACCTATTTCCCTGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205252 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MASEALHQVGDGEEAVLKKENFNMMNALDQLPKPFPNPKSMNRTVTTKGLPLASKGNLVNFLEDDTINLL
  KPLPVEDSDCSSDETSISAFSSTLLNPIKLAVTQPNSSFFAGMLEGELNKLSFSPMAKNAENEDLALGPC
  PCPSKSQMATRGLLDLDNPELETETSSTHSESSVVVDLPDTPFIFEHTVNNSTAVISWTYALGKQPVSFY
  QLLLQEVAKTQENELPEAKNRPWIFNKILGTTVKLMELKPNTCYCLSVRAANTAGVGKWCKPYKFATLAT
  DFSSFPENYPIQITVRRKEPRQKIVSIGPEEMRRLEDLEYLFPC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017559
• ORF Size: 972 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017559.1, NM_017559.2, NP_060029.1
• RefSeq Size: 1369 bp
• RefSeq ORF: 975 bp
• Locus ID: 54752
• MW: 35.9 kDa