INSIG2 (NM_016133) Human Tagged ORF Clone
CAT#: RC205274
INSIG2 (Myc-DDK-tagged)-Human insulin induced gene 2 (INSIG2)
"NM_016133" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | INSIG2 |
Synonyms | INSIG-2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC205274 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGAAGGAGAGACAGAGTCACCTGGGCCCAAAAAGTGTGGCCCATATATTTCATCTGTCACTAGCC AGAGTGTGAACTTGATGATTCGAGGAGTAGTGCTATTTTTTATTGGAGTATTTCTTGCATTAGTGTTAAA TTTACTTCAGATTCAGAGAAATGTGACGCTCTTTCCACCTGATGTGATTGCAAGCATCTTTTCTTCTGCA TGGTGGGTACCCCCATGCTGTGGCACGGCTTCAGCTGTGATTGGGTTATTATACCCCTGCATTGACAGAC ATCTAGGAGAACCACATAAATTTAAAAGAGAGTGGTCCAGTGTAATGCGGTGTGTAGCAGTCTTTGTTGG TATAAATCATGCCAGTGCTAAAGTGGATTTCGATAACAACATACAGTTGTCTCTCACACTGGCTGCACTA TCCATTGGACTGTGGTGGACTTTTGATAGATCTAGAAGTGGTTTTGGCCTTGGAGTAGGAATTGCCTTCT TGGCAACTGTGGTCACTCAACTGCTAGTATATAATGGTGTTTACCAATATACATCTCCAGATTTCCTCTA TGTTCGTTCTTGGTTACCATGTATATTTTTTGCTGGAGGCATAACAATGGGAAACATTGGTCGACAACTG GCAATGTACGAATGTAAAGTTATCGCAGAAAAATCTCATCAGGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205274 protein sequence
Red=Cloning site Green=Tags(s) MAEGETESPGPKKCGPYISSVTSQSVNLMIRGVVLFFIGVFLALVLNLLQIQRNVTLFPPDVIASIFSSA WWVPPCCGTASAVIGLLYPCIDRHLGEPHKFKREWSSVMRCVAVFVGINHASAKVDFDNNIQLSLTLAAL SIGLWWTFDRSRSGFGLGVGIAFLATVVTQLLVYNGVYQYTSPDFLYVRSWLPCIFFAGGITMGNIGRQL AMYECKVIAEKSHQE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_016133 |
ORF Size | 675 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_016133.2, NM_016133.3, NP_057217.2 |
RefSeq Size | 2592 bp |
RefSeq ORF | 678 bp |
Locus ID | 51141 |
Protein Families | Transmembrane |
MW | 24.8 kDa |
Gene Summary | The protein encoded by this gene is highly similar to the protein product encoded by gene INSIG1. Both INSIG1 protein and this protein are endoplasmic reticulum proteins that block the processing of sterol regulatory element binding proteins (SREBPs) by binding to SREBP cleavage-activating protein (SCAP), and thus prevent SCAP from escorting SREBPs to the Golgi. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC101330 | INSIG2 (untagged)-Human insulin induced gene 2 (INSIG2) |
USD 310.00 |
|
RG205274 | INSIG2 (GFP-tagged) - Human insulin induced gene 2 (INSIG2) |
USD 460.00 |
|
RC205274L1 | Lenti ORF clone of Human insulin induced gene 2 (INSIG2), Myc-DDK-tagged |
USD 768.00 |
|
RC205274L2 | Lenti ORF clone of Human insulin induced gene 2 (INSIG2), mGFP tagged |
USD 620.00 |
|
RC205274L3 | Lenti ORF clone of Human insulin induced gene 2 (INSIG2), Myc-DDK-tagged |
USD 768.00 |
|
RC205274L4 | Lenti ORF clone of Human insulin induced gene 2 (INSIG2), mGFP tagged |
USD 620.00 |
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