PI 3 Kinase Class 3 (PIK3C3) (NM_002647) Human Tagged ORF Clone
CAT#: RC205317
PIK3C3 (Myc-DDK-tagged)-Human phosphoinositide-3-kinase, class 3 (PIK3C3)
"NM_002647" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | PIK3C3 |
Synonyms | hVps34; VPS34; Vps34 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC205317 representing NM_002647
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGAAGCAGAGAAGTTTCACTACATCTATAGTTGTGACCTGGATATCAACGTCCAGCTTAAGATAG GAAGCTTGGAAGGGAAGAGAGAACAAAAGAGTTATAAAGCTGTCCTGGAAGACCCAATGTTGAAGTTCTC AGGACTATATCAAGAGACATGCTCTGATCTTTATGTTACTTGTCAAGTTTTTGCAGAAGGGAAGCCTTTG GCCTTGCCAGTGAGAACATCCTACAAAGCATTTAGTACAAGATGGAACTGGAATGAATGGCTGAAACTAC CAGTAAAATACCCTGACCTGCCCAGGAATGCCCAAGTGGCCCTCACCATATGGGATGTGTATGGTCCCGG AAAAGCAGTGCCTGTAGGAGGAACAACGGTTTCGCTCTTTGGAAAATACGGCATGTTTCGCCAAGGGATG CATGACTTGAAAGTCTGGCCTAATGTAGAAGCAGATGGATCAGAACCCACAAAAACTCCTGGCAGAACAA GTAGCACTCTCTCAGAAGATCAGATGAGCCGTCTTGCCAAGCTCACCAAAGCTCATCGACAAGGACACAT GGTGAAAGTAGATTGGCTGGATAGATTGACATTTAGAGAAATAGAAATGATAAATGAGAGTGAAAAACGA AGTTCTAATTTCATGTACCTGATGGTTGAATTTCGATGTGTCAAGTGTGATGATAAGGAATATGGTATTG TTTATTATGAAAAGGACGGTGATGAATCATCTCCAATTTTAACAAGTTTTGAATTAGTGAAAGTTCCTGA CCCCCAGATGTCTATGGAGAATTTAGTTGAGAGCAAACACCACAAGCTTGCCCGGAGTTTAAGAAGTGGA CCTTCTGACCACGATCTGAAACCCAATGCTGCCACGAGAGATCAGTTAAATATTATTGTGAGTTATCCAC CAACCAAGCAACTTACATATGAAGAACAAGATCTTGTTTGGAAGTTTAGATATTATCTTACGAATCAAGA AAAAGCCTTGACAAAATTCTTGAAATGTGTTAATTGGGATCTACCTCAAGAGGCCAAACAGGCCTTGGAA CTTCTGGGAAAATGGAAGCCGATGGATGTAGAGGACTCCTTGGAGCTGTTATCCTCTCATTACACCAACC CAACTGTGAGGCGTTATGCTGTTGCCCGGTTGCGACAGGCCGATGATGAGGATTTGTTGATGTACCTATT ACAATTGGTCCAGGCTCTCAAATATGAAAATTTTGATGATATAAAGAATGGATTGGAACCTACCAAGAAG GATAGTCAGAGTTCAGTGTCAGAAAATGTGTCAAATTCTGGAATAAATTCTGCAGAAATAGATAGCTCCC AAATTATAACCAGCCCCCTTCCTTCAGTCTCTTCACCTCCTCCTGCATCAAAAACAAAAGAAGTTCCAGA TGGCGAAAATCTGGAACAAGATCTCTGTACCTTCTTGATATCGAGAGCCTGCAAAAACTCAACACTGGCT AATTATTTATACTGGTATGTGATAGTGGAATGTGAAGATCAAGATACTCAGCAGAGAGATCCAAAGACCC ATGAGATGTACTTGAACGTAATGAGAAGATTCAGCCAAGCATTGTTGAAGGGTGATAAGTCTGTCAGAGT TATGCGTTCTTTGCTGGCTGCACAACAGACATTTGTAGATCGGTTGGTGCATCTAATGAAGGCAGTACAA CGCGAAAGTGGAAATCGTAAGAAAAAGAATGAGAGACTACAGGCATTGCTTGGAGATAATGAAAAGATGA ATTTGTCAGATGTGGAACTTATCCCGTTGCCTTTAGAACCCCAAGTGAAAATTAGAGGAATAATTCCGGA AACAGCTACACTGTTTAAAAGTGCCCTTATGCCTGCACAGTTGTTTTTTAAGACGGAAGATGGAGGCAAA TATCCAGTTATATTTAAGCATGGAGATGATTTACGTCAAGATCAACTTATTCTTCAAATCATTTCACTCA TGGACAAGCTGTTACGGAAAGAAAATCTGGACTTGAAATTGACACCTTATAAGGTGTTAGCCACCAGTAC AAAACATGGCTTCATGCAGTTTATCCAGTCAGTTCCTGTGGCTGAAGTTCTTGATACAGAGGGAAGCATT CAGAACTTTTTTAGAAAATATGCACCAAGTGAGAATGGGCCAAATGGGATTAGTGCTGAGGTCATGGACA CTTACGTTAAAAGCTGTGCTGGATATTGCGTGATCACCTATATACTTGGAGTTGGAGACAGGCACCTGGA TAACCTTTTGCTAACAAAAACAGGCAAACTCTTCCACATAGACTTTGGATATATTTTGGGTCGGGATCCA AAGCCTCTTCCTCCACCAATGAAGCTGAATAAAGAAATGGTAGAAGGAATGGGGGGCACACAGAGTGAGC AGTACCAAGAGTTCCGTAAACAGTGTTACACGGCTTTCCTCCACCTGCGAAGGTATTCTAATCTGATTTT GAACTTGTTTTCCTTGATGGTTGATGCAAACATTCCAGATATTGCACTTGAACCAGATAAAACTGTGAAA AAGGTTCAGGATAAATTCCGCTTAGACCTGTCGGATGAAGAGGCTGTGCATTACATGCAGAGTCTGATTG ATGAGAGTGTCCATGCTCTTTTTGCTGCAGTGGTGGAACAGATTCACAAGTTTGCCCAGTACTGGAGAAA A ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205317 representing NM_002647
Red=Cloning site Green=Tags(s) MGEAEKFHYIYSCDLDINVQLKIGSLEGKREQKSYKAVLEDPMLKFSGLYQETCSDLYVTCQVFAEGKPL ALPVRTSYKAFSTRWNWNEWLKLPVKYPDLPRNAQVALTIWDVYGPGKAVPVGGTTVSLFGKYGMFRQGM HDLKVWPNVEADGSEPTKTPGRTSSTLSEDQMSRLAKLTKAHRQGHMVKVDWLDRLTFREIEMINESEKR SSNFMYLMVEFRCVKCDDKEYGIVYYEKDGDESSPILTSFELVKVPDPQMSMENLVESKHHKLARSLRSG PSDHDLKPNAATRDQLNIIVSYPPTKQLTYEEQDLVWKFRYYLTNQEKALTKFLKCVNWDLPQEAKQALE LLGKWKPMDVEDSLELLSSHYTNPTVRRYAVARLRQADDEDLLMYLLQLVQALKYENFDDIKNGLEPTKK DSQSSVSENVSNSGINSAEIDSSQIITSPLPSVSSPPPASKTKEVPDGENLEQDLCTFLISRACKNSTLA NYLYWYVIVECEDQDTQQRDPKTHEMYLNVMRRFSQALLKGDKSVRVMRSLLAAQQTFVDRLVHLMKAVQ RESGNRKKKNERLQALLGDNEKMNLSDVELIPLPLEPQVKIRGIIPETATLFKSALMPAQLFFKTEDGGK YPVIFKHGDDLRQDQLILQIISLMDKLLRKENLDLKLTPYKVLATSTKHGFMQFIQSVPVAEVLDTEGSI QNFFRKYAPSENGPNGISAEVMDTYVKSCAGYCVITYILGVGDRHLDNLLLTKTGKLFHIDFGYILGRDP KPLPPPMKLNKEMVEGMGGTQSEQYQEFRKQCYTAFLHLRRYSNLILNLFSLMVDANIPDIALEPDKTVK KVQDKFRLDLSDEEAVHYMQSLIDESVHALFAAVVEQIHKFAQYWRK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_002647 |
ORF Size | 2661 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_002647.2, NM_002647.3, NP_002638.2 |
RefSeq Size | 3083 bp |
RefSeq ORF | 2664 bp |
Locus ID | 5289 |
Cytogenetics | 18q12.3 |
Domains | PI3_PI4_kinase, PI3Ka, PI3K_C2 |
Protein Families | Druggable Genome |
Protein Pathways | Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system, Regulation of autophagy |
MW | 101.4 kDa |
Gene Summary | '' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC118487 | PIK3C3 (untagged)-Human phosphoinositide-3-kinase, class 3 (PIK3C3) |
USD 310.00 |
|
RG205317 | PIK3C3 (GFP-tagged) - Human phosphoinositide-3-kinase, class 3 (PIK3C3) |
USD 830.00 |
|
RC205317L1 | Lenti ORF clone of Human phosphoinositide-3-kinase, class 3 (PIK3C3), Myc-DDK-tagged |
USD 1,176.00 |
|
RC205317L2 | Lenti ORF clone of Human phosphoinositide-3-kinase, class 3 (PIK3C3), mGFP tagged |
USD 950.00 |
|
RC205317L3 | Lenti ORF clone of Human phosphoinositide-3-kinase, class 3 (PIK3C3), Myc-DDK-tagged |
USD 950.00 |
|
RC205317L4 | Lenti ORF clone of Human phosphoinositide-3-kinase, class 3 (PIK3C3), mGFP tagged |
USD 950.00 |
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