PI 3 Kinase Class 3 (PIK3C3) (NM_002647) Human Tagged ORF Clone

CAT#: RG205317

PIK3C3 (GFP-tagged) - Human phosphoinositide-3-kinase, class 3 (PIK3C3)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PIK3C3
• Synonyms: hVps34; VPS34; Vps34
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG205317 representing NM_002647
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGAAGCAGAGAAGTTTCACTACATCTATAGTTGTGACCTGGATATCAACGTCCAGCTTAAGATAG
  GAAGCTTGGAAGGGAAGAGAGAACAAAAGAGTTATAAAGCTGTCCTGGAAGACCCAATGTTGAAGTTCTC
  AGGACTATATCAAGAGACATGCTCTGATCTTTATGTTACTTGTCAAGTTTTTGCAGAAGGGAAGCCTTTG
  GCCTTGCCAGTGAGAACATCCTACAAAGCATTTAGTACAAGATGGAACTGGAATGAATGGCTGAAACTAC
  CAGTAAAATACCCTGACCTGCCCAGGAATGCCCAAGTGGCCCTCACCATATGGGATGTGTATGGTCCCGG
  AAAAGCAGTGCCTGTAGGAGGAACAACGGTTTCGCTCTTTGGAAAATACGGCATGTTTCGCCAAGGGATG
  CATGACTTGAAAGTCTGGCCTAATGTAGAAGCAGATGGATCAGAACCCACAAAAACTCCTGGCAGAACAA
  GTAGCACTCTCTCAGAAGATCAGATGAGCCGTCTTGCCAAGCTCACCAAAGCTCATCGACAAGGACACAT
  GGTGAAAGTAGATTGGCTGGATAGATTGACATTTAGAGAAATAGAAATGATAAATGAGAGTGAAAAACGA
  AGTTCTAATTTCATGTACCTGATGGTTGAATTTCGATGTGTCAAGTGTGATGATAAGGAATATGGTATTG
  TTTATTATGAAAAGGACGGTGATGAATCATCTCCAATTTTAACAAGTTTTGAATTAGTGAAAGTTCCTGA
  CCCCCAGATGTCTATGGAGAATTTAGTTGAGAGCAAACACCACAAGCTTGCCCGGAGTTTAAGAAGTGGA
  CCTTCTGACCACGATCTGAAACCCAATGCTGCCACGAGAGATCAGTTAAATATTATTGTGAGTTATCCAC
  CAACCAAGCAACTTACATATGAAGAACAAGATCTTGTTTGGAAGTTTAGATATTATCTTACGAATCAAGA
  AAAAGCCTTGACAAAATTCTTGAAATGTGTTAATTGGGATCTACCTCAAGAGGCCAAACAGGCCTTGGAA
  CTTCTGGGAAAATGGAAGCCGATGGATGTAGAGGACTCCTTGGAGCTGTTATCCTCTCATTACACCAACC
  CAACTGTGAGGCGTTATGCTGTTGCCCGGTTGCGACAGGCCGATGATGAGGATTTGTTGATGTACCTATT
  ACAATTGGTCCAGGCTCTCAAATATGAAAATTTTGATGATATAAAGAATGGATTGGAACCTACCAAGAAG
  GATAGTCAGAGTTCAGTGTCAGAAAATGTGTCAAATTCTGGAATAAATTCTGCAGAAATAGATAGCTCCC
  AAATTATAACCAGCCCCCTTCCTTCAGTCTCTTCACCTCCTCCTGCATCAAAAACAAAAGAAGTTCCAGA
  TGGCGAAAATCTGGAACAAGATCTCTGTACCTTCTTGATATCGAGAGCCTGCAAAAACTCAACACTGGCT
  AATTATTTATACTGGTATGTGATAGTGGAATGTGAAGATCAAGATACTCAGCAGAGAGATCCAAAGACCC
  ATGAGATGTACTTGAACGTAATGAGAAGATTCAGCCAAGCATTGTTGAAGGGTGATAAGTCTGTCAGAGT
  TATGCGTTCTTTGCTGGCTGCACAACAGACATTTGTAGATCGGTTGGTGCATCTAATGAAGGCAGTACAA
  CGCGAAAGTGGAAATCGTAAGAAAAAGAATGAGAGACTACAGGCATTGCTTGGAGATAATGAAAAGATGA
  ATTTGTCAGATGTGGAACTTATCCCGTTGCCTTTAGAACCCCAAGTGAAAATTAGAGGAATAATTCCGGA
  AACAGCTACACTGTTTAAAAGTGCCCTTATGCCTGCACAGTTGTTTTTTAAGACGGAAGATGGAGGCAAA
  TATCCAGTTATATTTAAGCATGGAGATGATTTACGTCAAGATCAACTTATTCTTCAAATCATTTCACTCA
  TGGACAAGCTGTTACGGAAAGAAAATCTGGACTTGAAATTGACACCTTATAAGGTGTTAGCCACCAGTAC
  AAAACATGGCTTCATGCAGTTTATCCAGTCAGTTCCTGTGGCTGAAGTTCTTGATACAGAGGGAAGCATT
  CAGAACTTTTTTAGAAAATATGCACCAAGTGAGAATGGGCCAAATGGGATTAGTGCTGAGGTCATGGACA
  CTTACGTTAAAAGCTGTGCTGGATATTGCGTGATCACCTATATACTTGGAGTTGGAGACAGGCACCTGGA
  TAACCTTTTGCTAACAAAAACAGGCAAACTCTTCCACATAGACTTTGGATATATTTTGGGTCGGGATCCA
  AAGCCTCTTCCTCCACCAATGAAGCTGAATAAAGAAATGGTAGAAGGAATGGGGGGCACACAGAGTGAGC
  AGTACCAAGAGTTCCGTAAACAGTGTTACACGGCTTTCCTCCACCTGCGAAGGTATTCTAATCTGATTTT
  GAACTTGTTTTCCTTGATGGTTGATGCAAACATTCCAGATATTGCACTTGAACCAGATAAAACTGTGAAA
  AAGGTTCAGGATAAATTCCGCTTAGACCTGTCGGATGAAGAGGCTGTGCATTACATGCAGAGTCTGATTG
  ATGAGAGTGTCCATGCTCTTTTTGCTGCAGTGGTGGAACAGATTCACAAGTTTGCCCAGTACTGGAGAAA
  A
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG205317 representing NM_002647
  Red=Cloning site Green=Tags(s)
  
  MGEAEKFHYIYSCDLDINVQLKIGSLEGKREQKSYKAVLEDPMLKFSGLYQETCSDLYVTCQVFAEGKPL
  ALPVRTSYKAFSTRWNWNEWLKLPVKYPDLPRNAQVALTIWDVYGPGKAVPVGGTTVSLFGKYGMFRQGM
  HDLKVWPNVEADGSEPTKTPGRTSSTLSEDQMSRLAKLTKAHRQGHMVKVDWLDRLTFREIEMINESEKR
  SSNFMYLMVEFRCVKCDDKEYGIVYYEKDGDESSPILTSFELVKVPDPQMSMENLVESKHHKLARSLRSG
  PSDHDLKPNAATRDQLNIIVSYPPTKQLTYEEQDLVWKFRYYLTNQEKALTKFLKCVNWDLPQEAKQALE
  LLGKWKPMDVEDSLELLSSHYTNPTVRRYAVARLRQADDEDLLMYLLQLVQALKYENFDDIKNGLEPTKK
  DSQSSVSENVSNSGINSAEIDSSQIITSPLPSVSSPPPASKTKEVPDGENLEQDLCTFLISRACKNSTLA
  NYLYWYVIVECEDQDTQQRDPKTHEMYLNVMRRFSQALLKGDKSVRVMRSLLAAQQTFVDRLVHLMKAVQ
  RESGNRKKKNERLQALLGDNEKMNLSDVELIPLPLEPQVKIRGIIPETATLFKSALMPAQLFFKTEDGGK
  YPVIFKHGDDLRQDQLILQIISLMDKLLRKENLDLKLTPYKVLATSTKHGFMQFIQSVPVAEVLDTEGSI
  QNFFRKYAPSENGPNGISAEVMDTYVKSCAGYCVITYILGVGDRHLDNLLLTKTGKLFHIDFGYILGRDP
  KPLPPPMKLNKEMVEGMGGTQSEQYQEFRKQCYTAFLHLRRYSNLILNLFSLMVDANIPDIALEPDKTVK
  KVQDKFRLDLSDEEAVHYMQSLIDESVHALFAAVVEQIHKFAQYWRK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002647
• ORF Size: 2661 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002647.2, NP_002638.2
• RefSeq Size: 3083 bp
• RefSeq ORF: 2664 bp
• Locus ID: 5289
• Cytogenetics: 18q12.3
• Domains: PI3_PI4_kinase, PI3Ka, PI3K_C2
• Protein Families: Druggable Genome
• Protein Pathways: Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system, Regulation of autophagy
• Gene Summary: ''