KCNK1 (NM_002245) Human Tagged ORF Clone

CAT#: RC205321

KCNK1 (Myc-DDK-tagged)-Human potassium channel, subfamily K, member 1 (KCNK1)

Product Images

Western blot validation of overexpression lysate (Cat# LY400813) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205321 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KCNK1
• Synonyms: DPK; HOHO; K2P1; K2p1.1; KCNO1; TWIK-1; TWIK1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205321 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCAGTCCCTGGCCGGCAGCTCGTGCGTGCGCCTGGTGGAGCGGCACCGCTCGGCCTGGTGCTTCG
  GCTTCCTGGTGCTGGGCTACTTGCTCTACCTGGTCTTCGGCGCAGTGGTCTTCTCCTCGGTGGAGCTGCC
  CTATGAGGACCTGCTGCGCCAGGAGCTGCGCAAGCTGAAGCGACGCTTCTTGGAGGAGCACGAGTGCCTG
  TCTGAGCAGCAGCTGGAGCAGTTCCTGGGCCGGGTGCTGGAGGCCAGCAACTACGGCGTGTCGGTGCTCA
  GCAACGCCTCGGGCAACTGGAACTGGGACTTCACCTCCGCGCTCTTCTTCGCCAGCACCGTGCTCTCCAC
  CACAGGTTATGGCCACACCGTGCCCTTGTCAGATGGAGGTAAGGCCTTCTGCATCATCTACTCCGTCATT
  GGCATTCCCTTCACCCTCCTGTTCCTGACGGCTGTGGTCCAGCGCATCACCGTGCACGTCACCCGCAGGC
  CGGTCCTCTACTTCCACATCCGCTGGGGCTTCTCCAAGCAGGTGGTGGCCATCGTCCATGCCGTGCTCCT
  TGGGTTTGTCACTGTGTCCTGCTTCTTCTTCATCCCGGCCGCTGTCTTCTCAGTCCTGGAGGATGACTGG
  AACTTCCTGGAATCCTTTTATTTTTGTTTTATTTCCCTGAGCACCATTGGCCTGGGGGATTATGTGCCTG
  GGGAAGGCTACAATCAAAAATTCAGAGAGCTCTATAAGATTGGGATCACGTGTTACCTGCTACTTGGCCT
  TATTGCCATGTTGGTAGTTCTGGAAACCTTCTGTGAACTCCATGAGCTGAAAAAATTCAGAAAAATGTTC
  TATGTGAAGAAGGACAAGGACGAGGATCAGGTGCACATCATAGAGCATGACCAACTGTCATTCTCCTCGA
  TCACAGACCAGGCAGCTGGCATGAAAGAGGACCAGAAGCAAAATGAGCCTTTTGTGGCCACCCAGTCATC
  TGCCTGCGTGGATGGCCCTGCAAACCAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205321 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLQSLAGSSCVRLVERHRSAWCFGFLVLGYLLYLVFGAVVFSSVELPYEDLLRQELRKLKRRFLEEHECL
  SEQQLEQFLGRVLEASNYGVSVLSNASGNWNWDFTSALFFASTVLSTTGYGHTVPLSDGGKAFCIIYSVI
  GIPFTLLFLTAVVQRITVHVTRRPVLYFHIRWGFSKQVVAIVHAVLLGFVTVSCFFFIPAAVFSVLEDDW
  NFLESFYFCFISLSTIGLGDYVPGEGYNQKFRELYKIGITCYLLLGLIAMLVVLETFCELHELKKFRKMF
  YVKKDKDEDQVHIIEHDQLSFSSITDQAAGMKEDQKQNEPFVATQSSACVDGPANH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002245
• ORF Size: 1008 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002245.1, NM_002245.2, NM_002245.3, NP_002236.1
• RefSeq Size: 2161 bp
• RefSeq ORF: 1011 bp
• Locus ID: 3775
• Cytogenetics: 1q42.2
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• MW: 38.1 kDa
• Gene Summary: 'This gene encodes one of the members of the superfamily of potassium channel proteins containing two pore-forming P domains. The product of this gene has not been shown to be a functional channel, however, it may require other non-pore-forming proteins for activity. [provided by RefSeq, Jul 2008]'