Calponin (CNN1) (NM_001299) Human Tagged ORF Clone

CAT#: RC205370

CNN1 (Myc-DDK-tagged)-Human calponin 1, basic, smooth muscle (CNN1)

Product Images

Western blot validation of overexpression lysate (Cat# LY400519) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205370 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CNN1 protein (Cat# TP305370). The protein was produced from HEK293T cells transfected with CNN1 cDNA clone (Cat# RC205370) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CNN1
• Synonyms: HEL-S-14; Sm-Calp; SMCC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205370 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCTCTGCTCACTTCAACCGAGGCCCTGCCTACGGGCTGTCAGCCGAGGTTAAGAACAAGCTGGCCC
  AGAAGTATGACCACCAGCGGGAGCAGGAGCTGAGAGAGTGGATCGAGGGGGTGACAGGCCGTCGCATCGG
  CAACAACTTCATGGACGGCCTCAAAGATGGCATCATTCTTTGCGAATTCATCAATAAGCTGCAGCCAGGC
  TCCGTGAAGAAGATCAATGAGTCAACCCAAAATTGGCACCAGCTGGAGAACATCGGCAACTTCATCAAGG
  CCATCACCAAGTATGGGGTGAAGCCCCACGACATTTTTGAGGCCAACGACCTGTTTGAGAACACCAACCA
  TACACAGGTGCAGTCCACCCTCCTGGCTTTGGCCAGCATGGCGAAGACGAAAGGAAACAAGGTGAACGTG
  GGAGTGAAGTACGCAGAGAAGCAGGAGCGGAAATTCGAGCCGGGGAAGCTAAGAGAAGGGCGGAACATCA
  TTGGGCTGCAGATGGGCACCAACAAGTTTGCCAGCCAGCAGGGCATGACGGCCTATGGCACCCGGCGCCA
  CCTCTACGACCCCAAGCTGGGCACAGACCAGCCTCTGGACCAGGCGACCATCAGCCTGCAGATGGGCACC
  AACAAAGGAGCCAGCCAGGCTGGCATGACTGCGCCAGGGACCAAGCGGCAGATCTTCGAGCCGGGGCTGG
  GCATGGAGCACTGCGACACGCTCAATGTCAGCCTGCAGATGGGCAGCAACAAGGGCGCCTCGCAGCGGGG
  CATGACGGTGTATGGGCTGCCACGCCAGGTCTACGACCCCAAGTACTGTCTGACTCCCGAGTACCCAGAG
  CTGGGTGAGCCCGCCCACAACCACCACGCACACAACTACTACAATTCCGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205370 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSSAHFNRGPAYGLSAEVKNKLAQKYDHQREQELREWIEGVTGRRIGNNFMDGLKDGIILCEFINKLQPG
  SVKKINESTQNWHQLENIGNFIKAITKYGVKPHDIFEANDLFENTNHTQVQSTLLALASMAKTKGNKVNV
  GVKYAEKQERKFEPGKLREGRNIIGLQMGTNKFASQQGMTAYGTRRHLYDPKLGTDQPLDQATISLQMGT
  NKGASQAGMTAPGTKRQIFEPGLGMEHCDTLNVSLQMGSNKGASQRGMTVYGLPRQVYDPKYCLTPEYPE
  LGEPAHNHHAHNYYNSA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001299
• ORF Size: 891 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001299.1, NM_001299.2, NM_001299.3, NM_001299.4, NM_001299.5, NP_001290.2
• RefSeq Size: 1599 bp
• RefSeq ORF: 894 bp
• Locus ID: 1264
• Cytogenetics: 19p13.2
• Domains: calponin, CH
• MW: 33.2 kDa
• Gene Summary: ''